ID   PROT_VITXG              Reviewed;         769 AA.
AC   A0A2T4VDP8;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   18-JUL-2018, sequence version 1.
DT   03-AUG-2022, entry version 13.
DE   RecName: Full=Probable protease Ga0334635_1659 {ECO:0000305};
DE            EC=3.4.-.- {ECO:0000305|PubMed:35025633};
GN   ORFNames=DAT35_31120 {ECO:0000303|Ref.1},
GN   Ga0334635_1659 {ECO:0000303|PubMed:35025633};
OS   Vitiosangium sp. (strain GDMCC 1.1324).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC   Cystobacterineae; Archangiaceae; Vitiosangium; unclassified Vitiosangium.
OX   NCBI_TaxID=2138576;
RN   [1] {ECO:0000312|EMBL:PTL79886.1, ECO:0000312|Proteomes:UP000240889}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GDMCC 1.1324;
RA   Lv Y.;
RT   "Vitiosangium phaeum 53X41T novel myxobacteria isolated from forest.";
RL   Submitted (APR-2018) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION.
RC   STRAIN=GDMCC 1.1324;
RX   PubMed=35025633; DOI=10.1126/science.abj8432;
RA   Johnson A.G., Wein T., Mayer M.L., Duncan-Lowey B., Yirmiya E.,
RA   Oppenheimer-Shaanan Y., Amitai G., Sorek R., Kranzusch P.J.;
RT   "Bacterial gasdermins reveal an ancient mechanism of cell death.";
RL   Science 375:221-225(2022).
CC   -!- FUNCTION: Probably a dedicated protease for substrate gasdermin bGSDM;
CC       cleaves the bGSDM precursor, releasing the pore-forming moiety, which
CC       integrates into the membrane and triggers cell death. Involved in
CC       defense against bacteriophages (Probable). Expression of gasdermin
CC       bGSDM and this neighboring protease is toxic in E.coli
CC       (PubMed:35025633). {ECO:0000269|PubMed:35025633,
CC       ECO:0000305|PubMed:35025633}.
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DR   EMBL; PZOX01000012; PTL79886.1; -; Genomic_DNA.
DR   EnsemblBacteria; PTL79886; PTL79886; DAT35_31120.
DR   Proteomes; UP000240889; Unassembled WGS sequence.
DR   GO; GO:0008233; F:peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR024983; CHAT_dom.
DR   Pfam; PF12770; CHAT; 1.
PE   4: Predicted;
KW   Antiviral defense; Hydrolase; Protease; Reference proteome.
FT   CHAIN           1..769
FT                   /note="Probable protease Ga0334635_1659"
FT                   /id="PRO_0000455581"
FT   REGION          118..167
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        141..155
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   769 AA;  85379 MW;  EC6A2119F7266C0F CRC64;
     MSDDDKRHME LARLLVSLFP GRKLNGFLVG LLHSLSGRYR LPKELRGDRV PSSSKLARFI
     GTLDRERHIH SYFWDLLRRE RPSRVGDIDR VARLWEEEGH WKGLESLRFP AGEVFPQVAR
     GSSDNNGAPP LSFTLSHGDP KSDPEPSSPS RLVNTGLSEA ERPESPLASD QCLVTSHAYF
     FWLEVGAWIE GSIEDEPVSL PEDLPSGARL RVVLFSSPGG LVLQPDADQG VLCIEGDGRV
     VVERPACVPP ALQSRELADR RLFFPLRTPQ APGTYRLRCN IHHEGLLVQS RDVVLTVAEQ
     PQRQSEALRS RVDYALSQTL SPTHLRQLGS ATLSVLLNDD DWGTHGFRFV GGDDYRSSVH
     LDDAMLQEMI SLARGAFRRA SWGSKERYEA LPSRPPYRYQ GAQSEARLRE DLVMMARIGR
     QLYDRVAEQL GQGADGADAL RERMRLPGHI QLALKQGARH LVPISIFYDH RLDVALKDFT
     LCEAFIRASV ASEPLEKSPC FQGDCPHRED RDVVCPSGFW GFRHTLALPF GMAAQGERGL
     MDLPGVLRIR DTPALLIAVS EDPDFVLRDN HLMRLQAMSG VAPIQVARSR EKALELLRQQ
     GSHLVYFYCH GGVDAETRAP FLQVGPLTDP YIFRDTLRVY DIRWRDPAPH PLVFINGCHT
     TELEPEQAIE LVSGFVERAG AAGVIGTEVT IFEPLAVGFA SRFMDAFLRR RLSLGASVRE
     ARLGILQHDF NPLGLVYLPF ALSSLHLAGE ASEGTSVAGG AGALVTPPA