PROV_SALTY
ID PROV_SALTY Reviewed; 400 AA.
AC P17328;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2002, sequence version 2.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Glycine betaine/proline betaine transport system ATP-binding protein ProV {ECO:0000250|UniProtKB:P14175};
GN Name=proV {ECO:0000303|PubMed:2691838}; OrderedLocusNames=STM2809;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=LT2;
RX PubMed=2691838; DOI=10.1111/j.1365-2958.1989.tb00253.x;
RA Stirling D.A., Hulton C.S.J., Waddell L., Park S.F., Stewart G.S.A.B.,
RA Booth I.R., Higgins C.F.;
RT "Molecular characterization of the proU loci of Salmonella typhimurium and
RT Escherichia coli encoding osmoregulated glycine betaine transport
RT systems.";
RL Mol. Microbiol. 3:1025-1038(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-291.
RX PubMed=2548994; DOI=10.1128/jb.171.9.4694-4706.1989;
RA Overdier D.G., Olson E.R., Erickson B.D., Ederer M.M., Csonka L.N.;
RT "Nucleotide sequence of the transcriptional control region of the
RT osmotically regulated proU operon of Salmonella typhimurium and
RT identification of the 5' endpoint of the proU mRNA.";
RL J. Bacteriol. 171:4694-4706(1989).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-196.
RX PubMed=8195103; DOI=10.1128/jb.176.11.3420-3427.1994;
RA Jordan A., Gibert I., Barbe J.;
RT "Cloning and sequencing of the genes from Salmonella typhimurium encoding a
RT new bacterial ribonucleotide reductase.";
RL J. Bacteriol. 176:3420-3427(1994).
RN [5]
RP INDUCTION.
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=1423593; DOI=10.1016/0092-8674(92)90354-f;
RA Owen-Hughes T.A., Pavitt G.D., Santos D.S., Sidebotham J.M., Hulton C.S.,
RA Hinton J.C., Higgins C.F.;
RT "The chromatin-associated protein H-NS interacts with curved DNA to
RT influence DNA topology and gene expression.";
RL Cell 71:255-265(1992).
CC -!- FUNCTION: Part of the ProU ABC transporter complex involved in glycine
CC betaine and proline betaine uptake. Probably responsible for energy
CC coupling to the transport system. {ECO:0000250|UniProtKB:P14175}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (ProV),
CC two transmembrane proteins (ProW) and a solute-binding protein (ProX).
CC {ECO:0000250|UniProtKB:P14175}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000250|UniProtKB:P14175}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:P14175}.
CC -!- INDUCTION: Induced in response to increased extracellular osmolarity,
CC this is the first gene of the proU operon (proV-proW-proX).
CC Osmoregulation requires curved DNA downstream of the transcription
CC start site in this gene, which is repressed when bound by H-NS. H-NS
CC may act indirectly to influence the local topology of the promoter
CC (PubMed:1423593). {ECO:0000269|PubMed:1423593}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. {ECO:0000305}.
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DR EMBL; X52693; CAA36921.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL21694.1; -; Genomic_DNA.
DR EMBL; M26063; AAA88621.1; -; Genomic_DNA.
DR EMBL; X73226; CAA51696.1; -; Genomic_DNA.
DR PIR; S05374; QREBVT.
DR RefSeq; NP_461735.1; NC_003197.2.
DR RefSeq; WP_000985529.1; NC_003197.2.
DR AlphaFoldDB; P17328; -.
DR SMR; P17328; -.
DR STRING; 99287.STM2809; -.
DR PaxDb; P17328; -.
DR EnsemblBacteria; AAL21694; AAL21694; STM2809.
DR GeneID; 1254332; -.
DR KEGG; stm:STM2809; -.
DR PATRIC; fig|99287.12.peg.2967; -.
DR HOGENOM; CLU_000604_2_2_6; -.
DR OMA; GQIFVVM; -.
DR PhylomeDB; P17328; -.
DR BioCyc; SENT99287:STM2809-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR GO; GO:0071470; P:cellular response to osmotic stress; IDA:UniProtKB.
DR GO; GO:0031460; P:glycine betaine transport; IEA:InterPro.
DR Gene3D; 3.10.580.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR000644; CBS_dom.
DR InterPro; IPR046342; CBS_dom_sf.
DR InterPro; IPR005892; Gly-betaine_transp_ATP-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00005; ABC_tran; 1.
DR Pfam; PF00571; CBS; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF54631; SSF54631; 1.
DR TIGRFAMs; TIGR01186; proV; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51371; CBS; 2.
PE 2: Evidence at transcript level;
KW Amino-acid transport; ATP-binding; CBS domain; Cell inner membrane;
KW Cell membrane; Membrane; Nucleotide-binding; Reference proteome; Repeat;
KW Transport.
FT CHAIN 1..400
FT /note="Glycine betaine/proline betaine transport system
FT ATP-binding protein ProV"
FT /id="PRO_0000092762"
FT DOMAIN 29..265
FT /note="ABC transporter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT DOMAIN 280..341
FT /note="CBS 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT DOMAIN 343..400
FT /note="CBS 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT BINDING 61..68
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT CONFLICT 158
FT /note="A -> R (in Ref. 1; CAA36921)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 400 AA; 44125 MW; FDF58DE0A9A792C2 CRC64;
MAIKLEVKNL YKIFGEHPQR AFKYIEKGLS KEQILEKTGL SLGVKDASLA IEEGEIFVIM
GLSGSGKSTM VRLLNRLIEP TRGQVLIDGV DIAKISDAEL REVRRKKIAM VFQSFALMPH
MTVLDNTAFG MELAGIAAQE RREKALDALR QVGLENYAHA YPDELSGGMR QRVGLARALA
INPDILLMDE AFSALDPLIR TEMQDELVKL QAKHQRTIVF ISHDLDEAMR IGDRIAIMQN
GEVVQVGTPD EILNNPANDY VRTFFRGVDI SQVFSAKDIA RRSPVGLIRK TPGFGPRSAL
KLLQDEDREY GYVIERGNKF VGVVSIDSLK AALSQAQGIE AALIDDPLVV DAQTPLSELL
SHVGQAPCAV PVVDEEHQYV GIISKRMLLQ ALDREGGNNG
