ID   PROX_ECO57              Reviewed;         330 AA.
AC   P0AFM3; P14177;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Glycine betaine/proline betaine-binding periplasmic protein {ECO:0000250|UniProtKB:P0AFM2};
DE   Flags: Precursor;
GN   Name=proX {ECO:0000250|UniProtKB:P0AFM2}; OrderedLocusNames=Z3981, ECs3542;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Part of the ProU ABC transporter complex involved in glycine
CC       betaine and proline betaine uptake. Binds glycine betaine and proline
CC       betaine with high affinity. {ECO:0000250|UniProtKB:P0AFM2}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (ProV),
CC       two transmembrane proteins (ProW) and a solute-binding protein (ProX).
CC       {ECO:0000250|UniProtKB:P0AFM2}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000250|UniProtKB:P0AFM2}.
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DR   EMBL; AE005174; AAG57789.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB36965.1; -; Genomic_DNA.
DR   PIR; F91071; F91071.
DR   RefSeq; NP_311569.1; NC_002695.1.
DR   RefSeq; WP_001216525.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P0AFM3; -.
DR   SMR; P0AFM3; -.
DR   STRING; 155864.EDL933_3845; -.
DR   EnsemblBacteria; AAG57789; AAG57789; Z3981.
DR   EnsemblBacteria; BAB36965; BAB36965; ECs_3542.
DR   GeneID; 914742; -.
DR   KEGG; ece:Z3981; -.
DR   KEGG; ecs:ECs_3542; -.
DR   PATRIC; fig|386585.9.peg.3696; -.
DR   eggNOG; COG2113; Bacteria.
DR   HOGENOM; CLU_070055_0_0_6; -.
DR   OMA; EHNQGNY; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR   InterPro; IPR007210; ABC_Gly_betaine_transp_sub-bd.
DR   Pfam; PF04069; OpuAC; 1.
PE   3: Inferred from homology;
KW   Amino-acid transport; Disulfide bond; Periplasm; Reference proteome;
KW   Signal; Transport.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000250|UniProtKB:P0AFM2"
FT   CHAIN           22..330
FT                   /note="Glycine betaine/proline betaine-binding periplasmic
FT                   protein"
FT                   /id="PRO_0000045119"
FT   BINDING         86
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:P0AFM2"
FT   BINDING         90
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:P0AFM2"
FT   BINDING         161..163
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:P0AFM2"
FT   DISULFID        157..163
FT                   /evidence="ECO:0000250|UniProtKB:P0AFM2"
SQ   SEQUENCE   330 AA;  36023 MW;  2B98AF52552ACD2C CRC64;
     MRHSVLFATA FATLISTQTF AADLPGKGIT VNPVQSTITE ETFQTLLVSR ALEKLGYTVN
     KPSEVDYNVG YTSLASGDAT FTAVNWTPLH DNMYEAAGGD KKFYREGVFV NGAAQGYLID
     KKTADQYKIT NIAQLKDPKI AKLFDTNGDG KADLTGCNPG WGCEGAINHQ LAAYELTNTV
     THNQGNYAAM MADTISRYKE GKPVFYYTWT PYWVSNELKP GKDVVWLQVP FSALPGDKNA
     DTKLPNGANY GFPVSTMHIV ANKAWAEKNP AAAKLFAIMQ LPVADINAQN AIMHDGKASE
     GDIQGHVDGW IKAHQQQFDG WVNEALAAQK