ID   PROX_SALTY              Reviewed;         331 AA.
AC   Q8ZML1;
DT   06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Glycine betaine/proline betaine-binding periplasmic protein;
DE   AltName: Full=GBBP;
DE   Flags: Precursor;
GN   Name=proX; OrderedLocusNames=STM2811;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
RN   [2]
RP   INDUCTION.
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=1423593; DOI=10.1016/0092-8674(92)90354-f;
RA   Owen-Hughes T.A., Pavitt G.D., Santos D.S., Sidebotham J.M., Hulton C.S.,
RA   Hinton J.C., Higgins C.F.;
RT   "The chromatin-associated protein H-NS interacts with curved DNA to
RT   influence DNA topology and gene expression.";
RL   Cell 71:255-265(1992).
CC   -!- FUNCTION: Part of the ProU ABC transporter complex involved in glycine
CC       betaine and proline betaine uptake. Binds glycine betaine and proline
CC       betaine with high affinity. {ECO:0000250|UniProtKB:P0AFM2}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (ProV),
CC       two transmembrane proteins (ProW) and a solute-binding protein (ProX).
CC       {ECO:0000250|UniProtKB:P0AFM2}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000250|UniProtKB:P0AFM2}.
CC   -!- INDUCTION: Induced in response to increased extracellular osmolarity,
CC       this is the third gene of the proU operon (proV-proW-proX).
CC       Osmoregulation requires curved DNA downstream of the transcription
CC       start site in proV, which is repressed when bound by H-NS. H-NS may act
CC       indirectly to influence the local topology of the promoter
CC       (PubMed:1423593). {ECO:0000305|PubMed:1423593}.
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DR   EMBL; AE006468; AAL21696.1; -; Genomic_DNA.
DR   RefSeq; NP_461737.1; NC_003197.2.
DR   RefSeq; WP_001216622.1; NC_003197.2.
DR   AlphaFoldDB; Q8ZML1; -.
DR   SMR; Q8ZML1; -.
DR   STRING; 99287.STM2811; -.
DR   PaxDb; Q8ZML1; -.
DR   EnsemblBacteria; AAL21696; AAL21696; STM2811.
DR   GeneID; 1254334; -.
DR   KEGG; stm:STM2811; -.
DR   PATRIC; fig|99287.12.peg.2969; -.
DR   HOGENOM; CLU_070055_0_0_6; -.
DR   OMA; EHNQGNY; -.
DR   PhylomeDB; Q8ZML1; -.
DR   BioCyc; SENT99287:STM2811-MON; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR   InterPro; IPR007210; ABC_Gly_betaine_transp_sub-bd.
DR   Pfam; PF04069; OpuAC; 1.
PE   2: Evidence at transcript level;
KW   Amino-acid transport; Disulfide bond; Periplasm; Reference proteome;
KW   Signal; Transport.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..331
FT                   /note="Glycine betaine/proline betaine-binding periplasmic
FT                   protein"
FT                   /id="PRO_5004318332"
FT   BINDING         86
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:P0AFM2"
FT   BINDING         90
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:P0AFM2"
FT   BINDING         161..163
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:P0AFM2"
FT   DISULFID        157..163
FT                   /evidence="ECO:0000250|UniProtKB:P0AFM2"
SQ   SEQUENCE   331 AA;  36153 MW;  102CDDC0805936E1 CRC64;
     MRHTVIFASA FATLVTASAF AADLPGKGIT VQPIQSTISE ETFQTLLVSR ALEKLGYTVN
     KPSEVDYNVG YTSIASGDAT FTAVNWQPLH DDMYAAAGGD NKFYREGVFV SGAAQGYLID
     KKTAEQYNIT NIAQLKDPKI AKIFDTNGDG KADMMGCSPG WGCEAVINHQ NKAFDLQKTV
     EVSHGNYAAM MADTITRFKE GKPVLYYTWT PYWVSDVMKP GKDVVWLQVP FSSLPGEQKN
     IDTKLPNGAN YGFPVNTMHI VANKAWAEKN PAAAKLFAIM KLPLADINAQ NAMMHAGKSS
     EADVQGHVDG WINAHQQQFD GWVKEALAAQ K