ID   PRO_BPHK7               Reviewed;         225 AA.
AC   P49860;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Prohead protease {ECO:0000250|UniProtKB:Q6QGD7};
DE   AltName: Full=GP4;
GN   Name=4;
OS   Enterobacteria phage HK97 (Bacteriophage HK97).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae; Hendrixvirinae; Byrnievirus.
OX   NCBI_TaxID=2681617;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7723020; DOI=10.1016/s0022-2836(05)80144-5;
RA   Duda R.L., Martincic K., Hendrix R.W.;
RT   "Genetic basis of bacteriophage HK97 prohead assembly.";
RL   J. Mol. Biol. 247:636-647(1995).
CC   -!- FUNCTION: Protease involved in virion assembly and maturation.
CC       {ECO:0000250|UniProtKB:Q6QGD7}.
CC   -!- INTERACTION:
CC       P49860; P49861: 5; NbExp=3; IntAct=EBI-16087766, EBI-15611310;
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250|UniProtKB:Q6QGD7}.
CC       Note=Present in the capsid. {ECO:0000250|UniProtKB:Q6QGD7}.
CC   -!- PTM: Cleaves itself autocatalytically to yield the mature form of the
CC       protease. {ECO:0000250|UniProtKB:Q6QGD7}.
CC   -!- SIMILARITY: Belongs to the HK97 prohead protease protein family.
CC       {ECO:0000305}.
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DR   EMBL; U18319; AAA80203.1; -; Genomic_DNA.
DR   PIR; S54391; S54391.
DR   RefSeq; NP_037700.1; NC_002167.1.
DR   DIP; DIP-60656N; -.
DR   IntAct; P49860; 1.
DR   MEROPS; S78.001; -.
DR   GeneID; 1262531; -.
DR   KEGG; vg:1262531; -.
DR   GO; GO:0008233; F:peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   GO; GO:0046797; P:viral procapsid maturation; IEA:UniProtKB-KW.
DR   GO; GO:0019082; P:viral protein processing; IMP:CACAO.
DR   InterPro; IPR006433; Prohead_protease.
DR   Pfam; PF04586; Peptidase_S78; 1.
DR   TIGRFAMs; TIGR01543; proheadase_HK97; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Late protein; Protease; Viral capsid assembly;
KW   Viral capsid maturation; Viral release from host cell; Virion.
FT   CHAIN           1..225
FT                   /note="Prohead protease"
FT                   /id="PRO_0000065886"
FT   ACT_SITE        65
FT                   /evidence="ECO:0000250|UniProtKB:Q6QGD7"
FT   ACT_SITE        114
FT                   /evidence="ECO:0000250|UniProtKB:Q6QGD7"
FT   ACT_SITE        141
FT                   /evidence="ECO:0000250|UniProtKB:Q6QGD7"
SQ   SEQUENCE   225 AA;  24392 MW;  22CC899652160D11 CRC64;
     MPEIVKTLSF DETEIKFTGD GKQGIFEGYA SVFNNTDSDG DIILPGAFKN ALANQTRKVA
     MFFNHKTWEL PVGKWDSLAE DEKGLYVRGQ LTPGHSGAAD LKAAMQHGTV EGMSVGFSVA
     KDDYTIIPTG RIFKNIQALR EISVCTFPAN EQAGIAAMKS VDGIETIRDV ENWLRDSVGL
     TKSQAVGLIA RFKSAIRSES EGDGNEAQIN ALLQSIKSFP SNLGK