PRP18_MOUSE
ID PRP18_MOUSE Reviewed; 342 AA.
AC Q8BM39; Q3UX46; Q8BZ54;
DT 26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Pre-mRNA-splicing factor 18;
DE AltName: Full=PRP18 homolog;
GN Name=Prpf18; Synonyms=Prp18;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Bone, Egg, and Embryo;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Brain, and Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Participates in the second step of pre-mRNA splicing.
CC {ECO:0000250}.
CC -!- SUBUNIT: Heterodimer with PPIH. Interacts with PRPF4 and with the
CC spliceosome. Part of a complex containing U4/U6 snRNPs (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000250}. Note=Colocalizes
CC with spliceosomal snRNPs. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8BM39-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BM39-2; Sequence=VSP_008329, VSP_008330;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to an intron retention.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the PRP18 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC29527.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
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DR EMBL; AK035073; BAC28933.1; -; mRNA.
DR EMBL; AK036668; BAC29527.1; ALT_SEQ; mRNA.
DR EMBL; AK135707; BAE22619.1; -; mRNA.
DR EMBL; AK135890; BAE22717.1; -; mRNA.
DR EMBL; AK139720; BAE24112.1; -; mRNA.
DR EMBL; BC004573; AAH04573.1; -; mRNA.
DR EMBL; BC058631; AAH58631.1; -; mRNA.
DR CCDS; CCDS15657.1; -. [Q8BM39-1]
DR RefSeq; NP_080321.2; NM_026045.3. [Q8BM39-1]
DR AlphaFoldDB; Q8BM39; -.
DR BMRB; Q8BM39; -.
DR SMR; Q8BM39; -.
DR BioGRID; 212033; 22.
DR STRING; 10090.ENSMUSP00000041428; -.
DR iPTMnet; Q8BM39; -.
DR PhosphoSitePlus; Q8BM39; -.
DR EPD; Q8BM39; -.
DR MaxQB; Q8BM39; -.
DR PaxDb; Q8BM39; -.
DR PeptideAtlas; Q8BM39; -.
DR PRIDE; Q8BM39; -.
DR ProteomicsDB; 291664; -. [Q8BM39-1]
DR ProteomicsDB; 291665; -. [Q8BM39-2]
DR DNASU; 67229; -.
DR Ensembl; ENSMUST00000035721; ENSMUSP00000041428; ENSMUSG00000039449. [Q8BM39-1]
DR Ensembl; ENSMUST00000152362; ENSMUSP00000116495; ENSMUSG00000039449. [Q8BM39-1]
DR GeneID; 67229; -.
DR KEGG; mmu:67229; -.
DR UCSC; uc008iex.1; mouse. [Q8BM39-1]
DR CTD; 8559; -.
DR MGI; MGI:1914479; Prpf18.
DR VEuPathDB; HostDB:ENSMUSG00000039449; -.
DR eggNOG; KOG2808; Eukaryota.
DR GeneTree; ENSGT00390000015073; -.
DR HOGENOM; CLU_039675_0_1_1; -.
DR InParanoid; Q8BM39; -.
DR OMA; SFAQVRW; -.
DR OrthoDB; 1485522at2759; -.
DR PhylomeDB; Q8BM39; -.
DR TreeFam; TF315049; -.
DR BioGRID-ORCS; 67229; 12 hits in 71 CRISPR screens.
DR ChiTaRS; Prpf18; mouse.
DR PRO; PR:Q8BM39; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q8BM39; protein.
DR Bgee; ENSMUSG00000039449; Expressed in ear vesicle and 245 other tissues.
DR ExpressionAtlas; Q8BM39; baseline and differential.
DR Genevisible; Q8BM39; MM.
DR GO; GO:0016020; C:membrane; ISO:MGI.
DR GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR GO; GO:0071021; C:U2-type post-spliceosomal complex; IBA:GO_Central.
DR GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; IBA:GO_Central.
DR GO; GO:0005682; C:U5 snRNP; IBA:GO_Central.
DR GO; GO:0000350; P:generation of catalytic spliceosome for second transesterification step; IBA:GO_Central.
DR GO; GO:0051248; P:negative regulation of protein metabolic process; ISO:MGI.
DR Gene3D; 4.10.280.110; -; 1.
DR InterPro; IPR004098; Prp18.
DR InterPro; IPR014906; PRP4-like.
DR InterPro; IPR036285; PRP4-like_sf.
DR InterPro; IPR039979; PRPF18.
DR PANTHER; PTHR13007; PTHR13007; 1.
DR Pfam; PF02840; Prp18; 1.
DR Pfam; PF08799; PRP4; 1.
DR SMART; SM00500; SFM; 1.
DR SUPFAM; SSF158230; SSF158230; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; mRNA processing; mRNA splicing; Nucleus;
KW Reference proteome; Spliceosome.
FT CHAIN 1..342
FT /note="Pre-mRNA-splicing factor 18"
FT /id="PRO_0000058583"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q99633"
FT VAR_SEQ 171..175
FT /note="ALGES -> VVYPP (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_008329"
FT VAR_SEQ 176..342
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_008330"
FT CONFLICT 244
FT /note="A -> V (in Ref. 1; BAC29527)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 342 AA; 39890 MW; CF44DBB9D478ED99 CRC64;
MDILKSEILR KRQLVEDRNL LVENKKYFKR SELARKEEEA YYERCGYKIQ PKEDDQKPLT
SSNPVLELEL AEEKLPMTLS RQEVIRRLRE RGEPIRLFGE TDYDAFQRLR KIEILTPEVN
KGLRNDLKAA LDKIDQQYLN EIVGGQEPGE EDTQNDLKVH EENTTIEELE ALGESLGKGD
DHKDMDIITK FLKFLLGVWA KELNAREDYV KRSVQGKLNS ATQKQTESYL RPLFRKLRKR
NLPADIKESI TDIIKFMLQR EYVKANDAYL QMAIGNAPWP IGVTMVGIHA RTGREKIFSK
HVAHVLNDET QRKYIQGLKR LMTICQKHFP TDPSKCVEYN AL
