ID   PRP18_RAT               Reviewed;         342 AA.
AC   Q9JKB8;
DT   26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 120.
DE   RecName: Full=Pre-mRNA-splicing factor 18;
DE   AltName: Full=PRP18 homolog;
DE   AltName: Full=Potassium channel regulatory factor;
GN   Name=Prpf18; Synonyms=Kcrf, Prp18;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Heart atrium;
RX   PubMed=10924366; DOI=10.1006/bbrc.2000.3240;
RA   Keren-Raifman T., Ivanina T., Bismuth Y., Dascal N.;
RT   "Expression cloning of KCRF, a potassium channel regulatory factor.";
RL   Biochem. Biophys. Res. Commun. 274:852-858(2000).
CC   -!- FUNCTION: Participates in the second step of pre-mRNA splicing (By
CC       similarity). Down-regulates the expression of potassium channel
CC       subunits. {ECO:0000250}.
CC   -!- SUBUNIT: Heterodimer with PPIH. Interacts with PRPF4 and with the
CC       spliceosome. Part of a complex containing U4/U6 snRNPs (By similarity).
CC       Also detected in the cytoplasm. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000250}. Note=Colocalizes
CC       with spliceosomal snRNPs. {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Detected in brain, heart, liver and skeletal
CC       muscle.
CC   -!- SIMILARITY: Belongs to the PRP18 family. {ECO:0000305}.
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DR   EMBL; AF244920; AAF44715.1; -; mRNA.
DR   PIR; JC7358; JC7358.
DR   RefSeq; NP_612532.1; NM_138523.2.
DR   AlphaFoldDB; Q9JKB8; -.
DR   BMRB; Q9JKB8; -.
DR   SMR; Q9JKB8; -.
DR   STRING; 10116.ENSRNOP00000024872; -.
DR   PhosphoSitePlus; Q9JKB8; -.
DR   PaxDb; Q9JKB8; -.
DR   PRIDE; Q9JKB8; -.
DR   Ensembl; ENSRNOT00000024872; ENSRNOP00000024872; ENSRNOG00000018396.
DR   GeneID; 171552; -.
DR   KEGG; rno:171552; -.
DR   UCSC; RGD:708550; rat.
DR   CTD; 8559; -.
DR   RGD; 708550; Prpf18.
DR   eggNOG; KOG2808; Eukaryota.
DR   GeneTree; ENSGT00390000015073; -.
DR   HOGENOM; CLU_039675_0_1_1; -.
DR   InParanoid; Q9JKB8; -.
DR   OrthoDB; 1485522at2759; -.
DR   PhylomeDB; Q9JKB8; -.
DR   PRO; PR:Q9JKB8; -.
DR   Proteomes; UP000002494; Chromosome 17.
DR   Bgee; ENSRNOG00000018396; Expressed in Ammon's horn and 20 other tissues.
DR   ExpressionAtlas; Q9JKB8; baseline and differential.
DR   Genevisible; Q9JKB8; RN.
DR   GO; GO:0016020; C:membrane; IDA:RGD.
DR   GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR   GO; GO:0071021; C:U2-type post-spliceosomal complex; IBA:GO_Central.
DR   GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; IBA:GO_Central.
DR   GO; GO:0005682; C:U5 snRNP; IBA:GO_Central.
DR   GO; GO:0000350; P:generation of catalytic spliceosome for second transesterification step; IBA:GO_Central.
DR   Gene3D; 4.10.280.110; -; 1.
DR   InterPro; IPR004098; Prp18.
DR   InterPro; IPR014906; PRP4-like.
DR   InterPro; IPR036285; PRP4-like_sf.
DR   InterPro; IPR039979; PRPF18.
DR   PANTHER; PTHR13007; PTHR13007; 1.
DR   Pfam; PF02840; Prp18; 1.
DR   Pfam; PF08799; PRP4; 1.
DR   SMART; SM00500; SFM; 1.
DR   SUPFAM; SSF158230; SSF158230; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW   Spliceosome.
FT   CHAIN           1..342
FT                   /note="Pre-mRNA-splicing factor 18"
FT                   /id="PRO_0000058584"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0000250|UniProtKB:Q99633"
SQ   SEQUENCE   342 AA;  39866 MW;  0F094006BF1D2389 CRC64;
     MDILKSEILR KRQLVEDRNL LVENKKYFKR SELAKKEEEA YFERCGYKVQ PKEDDQKPLT
     SSNPVLELEL AEEKLPMTLS RQEVIRRLRE RGEPMRLFGE TDYDAFQRLR KIEILTPEVN
     KGLRNDLKAA LDKIDQQYLN EIVGGQEPGE EDTQNDLKVH EENTTIEELE ALGESLGKGD
     DHKDMDIITK FLKFLLGVWA KELNSREDYV KRSVQGKLNS ATQKQTESYL RPLFRKLRKR
     NLPADIKESI TDIIKFMLQR EYVKANDAYL QMAIGNAPWP IGVTMVGIHA RTGREKIFSK
     HVAHVLNDET QRKYIQGLKR LMTICQKHFP TDPSKCVEYN AL