PRP19_CAEEL
ID PRP19_CAEEL Reviewed; 492 AA.
AC Q10051;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 2.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Pre-mRNA-processing factor 19 {ECO:0000305};
DE EC=2.3.2.27 {ECO:0000250|UniProtKB:Q9UMS4};
DE AltName: Full=PRP19/PSO4 homolog;
DE AltName: Full=RING-type E3 ubiquitin transferase PRP19 {ECO:0000305};
GN Name=prp-19; ORFNames=T10F2.4;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=23149939; DOI=10.1128/mcb.01298-12;
RA Shiimori M., Inoue K., Sakamoto H.;
RT "A specific set of exon junction complex subunits is required for the
RT nuclear retention of unspliced RNAs in Caenorhabditis elegans.";
RL Mol. Cell. Biol. 33:444-456(2013).
CC -!- FUNCTION: Probable ubiquitin-protein ligase which is mainly involved
CC pre-mRNA splicing and DNA repair. Core component of the NTC/Nineteen
CC complex which is part of the spliceosome and participates in its
CC assembly, its remodeling and is required for its activity (By
CC similarity). Together with emb-4, necessary for interaction of rnp-4, a
CC probable exon junction complex component, with mRNAs and spliceosomal
CC snRNAs. Plays a role in nuclear retention of unspliced mRNAs.
CC {ECO:0000250|UniProtKB:Q9UMS4, ECO:0000269|PubMed:23149939}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000250|UniProtKB:Q9UMS4};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000250|UniProtKB:Q9UMS4}.
CC -!- SUBUNIT: Homotetramer. Component of the NTC complex (or PRP19-
CC associated complex) which is associated with the spliceosome.
CC {ECO:0000250|UniProtKB:Q9UMS4}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9UMS4}. Nucleus,
CC nucleoplasm {ECO:0000250|UniProtKB:Q9UMS4}.
CC -!- DISRUPTION PHENOTYPE: Enhances sterility conferred by rnp-4 disruption.
CC Combined with emb-4 knockdown, leads to accumulation and leakage of
CC unspliced ama-1 and tra-2 to the cytoplasm, severe growth retardation
CC and arrest in larval stages, and loss of interaction of rnp-4 with pre-
CC mRNA and spliceosomal U snRNAs. {ECO:0000269|PubMed:23149939}.
CC -!- SIMILARITY: Belongs to the WD repeat PRP19 family. {ECO:0000305}.
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DR EMBL; FO081619; CCD72869.1; -; Genomic_DNA.
DR PIR; T16846; T16846.
DR RefSeq; NP_001293643.1; NM_001306714.1.
DR AlphaFoldDB; Q10051; -.
DR SMR; Q10051; -.
DR DIP; DIP-26296N; -.
DR IntAct; Q10051; 2.
DR STRING; 6239.T10F2.4; -.
DR EPD; Q10051; -.
DR PaxDb; Q10051; -.
DR PeptideAtlas; Q10051; -.
DR EnsemblMetazoa; T10F2.4.1; T10F2.4.1; WBGene00020423.
DR UCSC; T10F2.4.1; c. elegans.
DR WormBase; T10F2.4; CE38104; WBGene00020423; prp-19.
DR eggNOG; KOG0289; Eukaryota.
DR GeneTree; ENSGT00940000153662; -.
DR HOGENOM; CLU_023894_1_1_1; -.
DR InParanoid; Q10051; -.
DR OMA; VTRVIYH; -.
DR OrthoDB; 1049599at2759; -.
DR PhylomeDB; Q10051; -.
DR Reactome; R-CEL-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-CEL-6782135; Dual incision in TC-NER.
DR Reactome; R-CEL-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR Reactome; R-CEL-72163; mRNA Splicing - Major Pathway.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q10051; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00020423; Expressed in embryo and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0000974; C:Prp19 complex; IBA:GO_Central.
DR GO; GO:0071006; C:U2-type catalytic step 1 spliceosome; IBA:GO_Central.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; ISS:UniProtKB.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0071028; P:nuclear mRNA surveillance; IMP:UniProtKB.
DR GO; GO:0070534; P:protein K63-linked ubiquitination; ISS:UniProtKB.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR013915; Pre-mRNA_splic_Prp19.
DR InterPro; IPR038959; Prp19.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR43995; PTHR43995; 1.
DR Pfam; PF08606; Prp19; 1.
DR Pfam; PF04564; U-box; 1.
DR Pfam; PF00400; WD40; 4.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00504; Ubox; 1.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS51698; U_BOX; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 4.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 3: Inferred from homology;
KW Developmental protein; Differentiation; DNA damage; DNA repair;
KW mRNA processing; mRNA splicing; Nucleus; Reference proteome; Repeat;
KW Spliceosome; Transferase; Ubl conjugation pathway; WD repeat.
FT CHAIN 1..492
FT /note="Pre-mRNA-processing factor 19"
FT /id="PRO_0000051148"
FT DOMAIN 1..72
FT /note="U-box"
FT REPEAT 207..246
FT /note="WD 1"
FT REPEAT 249..288
FT /note="WD 2"
FT REPEAT 291..330
FT /note="WD 3"
FT REPEAT 336..375
FT /note="WD 4"
FT REPEAT 378..417
FT /note="WD 5"
FT REPEAT 461..491
FT /note="WD 6"
SQ SEQUENCE 492 AA; 53208 MW; BAE2BC0522C9C0C9 CRC64;
MSFVCGISGE LTEDPVVSQV SGHIFDRRLI VKFIAENGTD PISHGELSED QLVSLKSGGT
GSAPRNVSGT SIPSLLKMLQ DEWDTVMLNS FSLRQQLQIA RQELSHSLYQ HDAACRVISR
LSKELTAARE ALSTLKPHTS AKVDDDVSID ESEDQQGLSE AILAKLEEKS KSLTAERKQR
GKNLPEGLAK TEELAELKQT ASHTGIHSTG TPGITALDIK GNLSLTGGID KTVVLYDYEK
EQVMQTFKGH NKKINAVVLH PDNITAISAS ADSHIRVWSA TDSSSKAIID VHQAPVTDIS
LNASGDYILS ASDDSYWAFS DIRSGKSLCK VSVEPGSQIA VHSIEFHPDG LIFGTGAADA
VVKIWDLKNQ TVAAAFPGHT AAVRSIAFSE NGYYLATGSE DGEVKLWDLR KLKNLKTFAN
EEKQPINSLS FDMTGTFLGI GGQKVQVLHV KSWSEVVSLS DHSGPVTGVR FGENARSLVT
CSLDKSLRVF SF
