PRP19_DICDI
ID PRP19_DICDI Reviewed; 514 AA.
AC Q7KWK5; Q550P2;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 116.
DE RecName: Full=Pre-mRNA-processing factor 19 {ECO:0000305};
DE EC=2.3.2.27 {ECO:0000250|UniProtKB:Q9UMS4};
DE AltName: Full=PRP19/PSO4 homolog;
DE AltName: Full=RING-type E3 ubiquitin transferase PRP19 {ECO:0000305};
GN Name=prp19; ORFNames=DDB_G0276803;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Probable ubiquitin-protein ligase mainly involved in pre-mRNA
CC splicing and DNA repair. Core component of the Prp19
CC complex/PRP19C/Nineteen complex/NTC which is part of the spliceosome
CC and participates in its assembly, its remodeling and is required for
CC its activity. {ECO:0000250|UniProtKB:Q9UMS4}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000250|UniProtKB:Q9UMS4};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000250|UniProtKB:Q9UMS4}.
CC -!- SUBUNIT: Homotetramer. Component of the NTC complex (or PRP19-
CC associated complex) which is associated with the spliceosome.
CC {ECO:0000250|UniProtKB:P32523, ECO:0000250|UniProtKB:Q9UMS4}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9UMS4}. Nucleus,
CC nucleoplasm {ECO:0000250|UniProtKB:Q9UMS4}.
CC -!- SIMILARITY: Belongs to the WD repeat PRP19 family. {ECO:0000305}.
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DR EMBL; AAFI02000019; EAL68903.1; -; Genomic_DNA.
DR RefSeq; XP_642890.1; XM_637798.1.
DR AlphaFoldDB; Q7KWK5; -.
DR SMR; Q7KWK5; -.
DR STRING; 44689.DDB0233121; -.
DR PaxDb; Q7KWK5; -.
DR EnsemblProtists; EAL68903; EAL68903; DDB_G0276803.
DR GeneID; 8620756; -.
DR KEGG; ddi:DDB_G0276803; -.
DR dictyBase; DDB_G0276803; prp19.
DR eggNOG; KOG0289; Eukaryota.
DR HOGENOM; CLU_023894_1_0_1; -.
DR InParanoid; Q7KWK5; -.
DR OMA; VTRVIYH; -.
DR PhylomeDB; Q7KWK5; -.
DR Reactome; R-DDI-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-DDI-6782135; Dual incision in TC-NER.
DR Reactome; R-DDI-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR Reactome; R-DDI-72163; mRNA Splicing - Major Pathway.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q7KWK5; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0000974; C:Prp19 complex; IBA:GO_Central.
DR GO; GO:0005681; C:spliceosomal complex; ISS:dictyBase.
DR GO; GO:0071006; C:U2-type catalytic step 1 spliceosome; IBA:GO_Central.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; ISS:UniProtKB.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IBA:GO_Central.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0070534; P:protein K63-linked ubiquitination; ISS:UniProtKB.
DR GO; GO:0000375; P:RNA splicing, via transesterification reactions; ISS:dictyBase.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR013915; Pre-mRNA_splic_Prp19.
DR InterPro; IPR038959; Prp19.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR43995; PTHR43995; 1.
DR Pfam; PF08606; Prp19; 1.
DR Pfam; PF00400; WD40; 2.
DR SMART; SM00504; Ubox; 1.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS51698; U_BOX; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 3: Inferred from homology;
KW DNA damage; DNA repair; mRNA processing; mRNA splicing; Nucleus;
KW Reference proteome; Repeat; Spliceosome; Transferase;
KW Ubl conjugation pathway; WD repeat.
FT CHAIN 1..514
FT /note="Pre-mRNA-processing factor 19"
FT /id="PRO_0000328058"
FT DOMAIN 1..70
FT /note="U-box"
FT REPEAT 214..254
FT /note="WD 1"
FT REPEAT 258..296
FT /note="WD 2"
FT REPEAT 307..346
FT /note="WD 3"
FT REPEAT 348..389
FT /note="WD 4"
FT REPEAT 392..430
FT /note="WD 5"
FT REPEAT 438..476
FT /note="WD 6"
FT REPEAT 481..514
FT /note="WD 7"
FT REGION 137..169
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 514 AA; 56811 MW; 4943067A45ACABFD CRC64;
MICAISGSTT EEPVISTKTG NVYEKRLIEK YIDTNGKEPT TGEPLGLSDL ITVKIGKTVK
PRPTTATSIP SMLQLFQNEW DSLMLETFTL KQQHETVRQE LAHSMYQYDA ACRVIARLVK
ERDAARSALA NARNIVQQQQ QQTNNNNNNN NNKDVEMSSA STPSSSEDNL LDSTIYKRIA
EKSEELIKTR KERTHQSLAE PDAIKQFKVS ANVETDCQIK SLEVNSQDNN LIAMGGINGN
VYVYSRETNE LCTAIKGTSS APINKVLFAP NNTIVSAGSD AVIRVVKGGE SPSKKTYTQY
KSTYQFAHKA SVTDISLHVL GDYIISSSLD KSLNMYDIVN GTHLTSFGQS QQLCYTSVAF
HPDGMFFAAG TQNGVVKIFD LKSKVNSFNF QGLSTAVNCI SFSENGYYLT AADNNTVKLF
DLRKAATKNS PDIQTITLDN QQIKSINFDY SSQYLGVASS NQINLYSCKS KQKPPISSIH
SIDSDCLDFK WARPNSNFFV TSSSSKNIVN IFSN
