PRP19_ORYSJ
ID PRP19_ORYSJ Reviewed; 527 AA.
AC Q9AV81; F4MGW6; Q7XDL9;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Pre-mRNA-processing factor 19 {ECO:0000305};
DE EC=2.3.2.27 {ECO:0000269|PubMed:19825583};
DE AltName: Full=Plant U-box protein 72;
DE Short=OsPUB72;
DE AltName: Full=RING-type E3 ubiquitin transferase PRP19 {ECO:0000305};
DE AltName: Full=U-box domain-containing protein 72;
GN Name=PRP19; Synonyms=PUB72; OrderedLocusNames=Os10g0466300, LOC_Os10g32880;
GN ORFNames=OsJ_31829, OSJNBa0006L06.5;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12791992; DOI=10.1126/science.1083523;
RA Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S.,
RA Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D.,
RA Oates R., Palmer M., Pries G., Gibson J., Anderson H., Paradkar M.,
RA Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F.,
RA Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M.,
RA Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R.,
RA Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F.,
RA Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D.,
RA Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R.,
RA Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K.,
RA Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S.,
RA Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S.,
RA Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R.,
RA Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M.,
RA Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R.,
RA Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E.,
RA Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A.;
RT "In-depth view of structure, activity, and evolution of rice chromosome
RT 10.";
RL Science 300:1566-1569(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP FUNCTION, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=19825583; DOI=10.1093/mp/ssn044;
RA Zeng L.R., Park C.H., Venu R.C., Gough J., Wang G.L.;
RT "Classification, expression pattern, and E3 ligase activity assay of rice
RT U-box-containing proteins.";
RL Mol. Plant 1:800-815(2008).
CC -!- FUNCTION: Probable ubiquitin-protein ligase which is mainly involved
CC pre-mRNA splicing and DNA repair. {ECO:0000250|UniProtKB:Q9UMS4,
CC ECO:0000269|PubMed:19825583}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000269|PubMed:19825583};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000269|PubMed:19825583}.
CC -!- SUBUNIT: Homotetramer. Component of the multiprotein assembly MOS4-
CC associated complex (MAC) at least composed of MOS4, CDC5, PRL1 and
CC PRP19 which is related to the PRP19C/Prp19 complex/NTC/Nineteen complex
CC identified in other organisms. Associated with the spliceosome.
CC {ECO:0000250|UniProtKB:Q94BR4}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q94BR4}.
CC -!- SIMILARITY: Belongs to the WD repeat PRP19 family. {ECO:0000305}.
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DR EMBL; AC022457; AAK27816.1; -; Genomic_DNA.
DR EMBL; DP000086; AAP54192.1; -; Genomic_DNA.
DR EMBL; DP000086; ABG66129.1; -; Genomic_DNA.
DR EMBL; AP008216; BAF26718.1; -; Genomic_DNA.
DR EMBL; AP014966; BAT11216.1; -; Genomic_DNA.
DR EMBL; CM000147; EEE51104.1; -; Genomic_DNA.
DR EMBL; AK064778; BAG89200.1; -; mRNA.
DR RefSeq; XP_015614850.1; XM_015759364.1.
DR AlphaFoldDB; Q9AV81; -.
DR SMR; Q9AV81; -.
DR STRING; 4530.OS10T0466300-01; -.
DR PaxDb; Q9AV81; -.
DR PRIDE; Q9AV81; -.
DR EnsemblPlants; Os10t0466300-01; Os10t0466300-01; Os10g0466300.
DR EnsemblPlants; Os10t0466300-02; Os10t0466300-02; Os10g0466300.
DR GeneID; 4348844; -.
DR Gramene; Os10t0466300-01; Os10t0466300-01; Os10g0466300.
DR Gramene; Os10t0466300-02; Os10t0466300-02; Os10g0466300.
DR KEGG; osa:4348844; -.
DR eggNOG; KOG0289; Eukaryota.
DR HOGENOM; CLU_023894_1_0_1; -.
DR InParanoid; Q9AV81; -.
DR OMA; VTRVIYH; -.
DR OrthoDB; 1049599at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000000763; Chromosome 10.
DR Proteomes; UP000007752; Chromosome 10.
DR Proteomes; UP000059680; Chromosome 10.
DR Genevisible; Q9AV81; OS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0000974; C:Prp19 complex; IBA:GO_Central.
DR GO; GO:0071006; C:U2-type catalytic step 1 spliceosome; IBA:GO_Central.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; ISS:UniProtKB.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IDA:UniProtKB.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0070534; P:protein K63-linked ubiquitination; ISS:UniProtKB.
DR GO; GO:0016567; P:protein ubiquitination; IDA:UniProtKB.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR013915; Pre-mRNA_splic_Prp19.
DR InterPro; IPR038959; Prp19.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR43995; PTHR43995; 1.
DR Pfam; PF08606; Prp19; 1.
DR Pfam; PF00400; WD40; 4.
DR SMART; SM00504; Ubox; 1.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS51698; U_BOX; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 4.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; DNA damage; DNA repair; Immunity; Innate immunity;
KW mRNA processing; mRNA splicing; Nucleus; Plant defense; Reference proteome;
KW Repeat; Spliceosome; Transferase; Ubl conjugation pathway; WD repeat.
FT CHAIN 1..527
FT /note="Pre-mRNA-processing factor 19"
FT /id="PRO_0000397692"
FT DOMAIN 1..70
FT /note="U-box"
FT REPEAT 219..258
FT /note="WD 1"
FT REPEAT 261..300
FT /note="WD 2"
FT REPEAT 306..345
FT /note="WD 3"
FT REPEAT 350..389
FT /note="WD 4"
FT REPEAT 392..430
FT /note="WD 5"
FT REPEAT 435..474
FT /note="WD 6"
FT REPEAT 481..520
FT /note="WD 7"
FT COILED 107..141
FT /evidence="ECO:0000255"
SQ SEQUENCE 527 AA; 57817 MW; EE8B08B9FC7AFDA5 CRC64;
MICAISGEVP DEPVVSKKSG LLFERRLVER YIEDHGKCPV TKEELTMDDI VAVKTNKVVK
PRQLQAASIP GLLGMFQNEW DAIMLSSFAL EQQLHTARQE LSHALYQHDA ACRVIARLKK
ERDEARALLA QAERQIPASM AGAAPTAVVS NGKRAFEDEV GPDGKKIRPG INPVMIDELT
ECNTMLSAHR KKRQVPPTLA SIDAIERYTQ ISSHPLHKTN KPGILSMDIH PSKDIIATGG
IDTNAVLFDR PSGQILCTLT GHSKKITSLK FVPRDELFVT GSADKTVKIW QGSEEGNYNC
IHTLKDHTAE VEAVTVHATQ KYFVTASKDN TWCFYDIPSG SCLTQVGESS GQEGYTSASF
HPDGLILGTG TTEAVVKIWD VKTQSNVAKF EGHVGPVTAM SFSENGYFLA TAALDGVKLW
DLRKLRNFRT ISPYDSDTPT NSVEFDFSGS YLAVGGSDTR VYQVANVKLE WNLVKTLPDL
SGTGKVTNVK FGTDAKYIAV GSMDRNLRIF GHPGEDDQMD DAKPSEE
