PRP19_SCHPO
ID PRP19_SCHPO Reviewed; 488 AA.
AC O14011;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 160.
DE RecName: Full=Pre-mRNA-processing factor 19 {ECO:0000305};
DE EC=2.3.2.27 {ECO:0000250|UniProtKB:P32523};
DE AltName: Full=Complexed with cdc5 protein 8;
DE AltName: Full=RING-type E3 ubiquitin transferase PRP19 {ECO:0000305};
GN Name=prp19; Synonyms=cwf8; ORFNames=SPAC29A4.08c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 99-113.
RX PubMed=10409726; DOI=10.1128/mcb.19.8.5352;
RA McDonald W.H., Ohi R., Smelkova N., Frendewey D., Gould K.L.;
RT "Myb-related fission yeast cdc5p is a component of a 40S snRNP-containing
RT complex and is essential for pre-mRNA splicing.";
RL Mol. Cell. Biol. 19:5352-5362(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP IDENTIFICATION IN THE CWF COMPLEX, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=11884590; DOI=10.1128/mcb.22.7.2011-2024.2002;
RA Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.;
RT "Proteomics analysis reveals stable multiprotein complexes in both fission
RT and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA
RT splicing factors, and snRNAs.";
RL Mol. Cell. Biol. 22:2011-2024(2002).
CC -!- FUNCTION: Probable ubiquitin-protein ligase involved in pre-mRNA
CC splicing where it associates with cdc5 and the other cwf proteins as
CC part of the spliceosome. May also function in DNA repair.
CC {ECO:0000250|UniProtKB:P32523, ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000250|UniProtKB:P32523};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000250|UniProtKB:P32523}.
CC -!- SUBUNIT: Homotetramer (By similarity). Component of the NTC complex (or
CC PRP19-associated complex), composed of at least CEF1, CLF1, ISY1,
CC NTC20, SNT309, SYF1, SYF2, and PRP19 (By similarity). Component of the
CC 40S cdc5-associated complex (or cwf complex), a spliceosome sub-complex
CC reminiscent of a late-stage spliceosome composed of the U2, U5 and U6
CC snRNAs and at least brr2, cdc5, cwf2/prp3, cwf3/syf1, cwf4/syf3,
CC cwf5/ecm2, spp42/cwf6, cwf7/spf27, cwf8, cwf9, cwf10, cwf11, cwf12,
CC prp45/cwf13, cwf14, cwf15, cwf16, cwf17, cwf18, cwf19, cwf20, cwf21,
CC cwf22, cwf23, cwf24, cwf25, cwf26, cyp7/cwf27, cwf28, cwf29/ist3, lea1,
CC msl1, prp5/cwf1, prp10, prp12/sap130, prp17, prp22, sap61, sap62,
CC sap114, sap145, slu7, smb1, smd1, smd3, smf1, smg1 and syf2. Interacts
CC with cdc5. {ECO:0000250|UniProtKB:P32523, ECO:0000269|PubMed:11884590}.
CC -!- INTERACTION:
CC O14011; Q09685: dre4; NbExp=6; IntAct=EBI-590830, EBI-4408349;
CC O14011; O43071: prp17; NbExp=5; IntAct=EBI-590830, EBI-538927;
CC O14011; O74517: saf1; NbExp=3; IntAct=EBI-590830, EBI-4421067;
CC O14011; Q9P7H6: SPAC1782.03; NbExp=2; IntAct=EBI-590830, EBI-4421106;
CC O14011; O14097: SPAC2F3.14c; NbExp=3; IntAct=EBI-590830, EBI-4420697;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the WD repeat PRP19 family. {ECO:0000305}.
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DR EMBL; AF254351; AAF67750.1; -; Genomic_DNA.
DR EMBL; CU329670; CAB10135.1; -; Genomic_DNA.
DR PIR; T38481; T38481.
DR RefSeq; NP_594874.1; NM_001020303.2.
DR PDB; 3JB9; EM; 3.60 A; S/T/U/V=1-488.
DR PDBsum; 3JB9; -.
DR AlphaFoldDB; O14011; -.
DR SMR; O14011; -.
DR BioGRID; 278726; 89.
DR DIP; DIP-34861N; -.
DR IntAct; O14011; 51.
DR STRING; 4896.SPAC29A4.08c.1; -.
DR MaxQB; O14011; -.
DR PaxDb; O14011; -.
DR EnsemblFungi; SPAC29A4.08c.1; SPAC29A4.08c.1:pep; SPAC29A4.08c.
DR GeneID; 2542256; -.
DR KEGG; spo:SPAC29A4.08c; -.
DR PomBase; SPAC29A4.08c; prp19.
DR VEuPathDB; FungiDB:SPAC29A4.08c; -.
DR eggNOG; KOG0289; Eukaryota.
DR HOGENOM; CLU_023894_0_1_1; -.
DR InParanoid; O14011; -.
DR OMA; VTRVIYH; -.
DR PhylomeDB; O14011; -.
DR Reactome; R-SPO-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-SPO-6782135; Dual incision in TC-NER.
DR Reactome; R-SPO-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR UniPathway; UPA00143; -.
DR PRO; PR:O14011; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005737; C:cytoplasm; HDA:PomBase.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005635; C:nuclear envelope; HDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0071014; C:post-mRNA release spliceosomal complex; IDA:PomBase.
DR GO; GO:0000974; C:Prp19 complex; IDA:PomBase.
DR GO; GO:0005681; C:spliceosomal complex; IDA:PomBase.
DR GO; GO:0071006; C:U2-type catalytic step 1 spliceosome; IBA:GO_Central.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; ISS:UniProtKB.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IBA:GO_Central.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0045292; P:mRNA cis splicing, via spliceosome; TAS:PomBase.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0070534; P:protein K63-linked ubiquitination; ISS:UniProtKB.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR024977; Apc4_WD40_dom.
DR InterPro; IPR013915; Pre-mRNA_splic_Prp19.
DR InterPro; IPR038959; Prp19.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR43995; PTHR43995; 2.
DR Pfam; PF12894; ANAPC4_WD40; 1.
DR Pfam; PF08606; Prp19; 1.
DR SMART; SM00504; Ubox; 1.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS51698; U_BOX; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; DNA damage; DNA repair;
KW mRNA processing; mRNA splicing; Nucleus; Reference proteome; Repeat;
KW Spliceosome; Transferase; Ubl conjugation pathway; WD repeat.
FT CHAIN 1..488
FT /note="Pre-mRNA-processing factor 19"
FT /id="PRO_0000050950"
FT DOMAIN 1..71
FT /note="U-box"
FT REPEAT 331..370
FT /note="WD 1"
FT REPEAT 373..413
FT /note="WD 2"
SQ SEQUENCE 488 AA; 54191 MW; 3900431C90A94A41 CRC64;
MFCSISGETP KEPVISRVSG NVYEKRLIEQ VIRETSKDPV TQQECTLEDL VPVKVPDFVR
PRPPSATSLP ALLSLFQEEW DSVALEQFEL RRNLTETKQE LSTALYSLDA ALRVISRLTK
ERDEAREALA KFSDNIGTVS SKTIEVQEVE MGESDDQLKT SLRSTVEKTF QELSSKRKRT
KLQPKWATDD AVSQLLQATP STILENLETE STTSFFPSPE NSSFVLCLHK DELLCLDIQS
NSTLKIFEGS ALACCWLTSS KIAVATADAI SIFEFPVSSS GLQSVGEPTS SIPIDEKVNF
LQAHPSGEYL LAASNEKCYI FSLKSQVYNI TVAQHITSLA VHPDGNLFVA GLENGELRFF
ETSSGNELTK FGPHSSPVKT LQFGENGYWL VVTTNDDSDI FIWDLRKSEL VQKIPLQTKV
AAVSLDITSQ LLVSSDGETL YVHIYVKSSK SWRCMSQTHV SSISNLVWLN ELHQLLFSTS
NGAILRLG
