PRP31_SCHPO
ID PRP31_SCHPO Reviewed; 518 AA.
AC O42904;
DT 11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Pre-mRNA-processing factor 31;
GN Name=prp31; ORFNames=SPBC119.13c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=10871341; DOI=10.1093/nar/28.11.2214;
RA Bishop D.T., McDonald W.H., Gould K.L., Forsburg S.L.;
RT "Isolation of an essential Schizosaccharomyces pombe gene, prp31+, that
RT links splicing and meiosis.";
RL Nucleic Acids Res. 28:2214-2220(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Required for spliceosome assembly where it is needed to link
CC the U4/U6XU5 tri-snRNP to the prespliceosome complexes.
CC {ECO:0000269|PubMed:10871341}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16823372}.
CC -!- DOMAIN: Interacts with the snRNP via the Nop domain. {ECO:0000250}.
CC -!- DOMAIN: The coiled coil domain is formed by two non-contiguous helices.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the PRP31 family. {ECO:0000305}.
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DR EMBL; CU329671; CAA17928.1; -; Genomic_DNA.
DR PIR; T39311; T39311.
DR RefSeq; NP_595294.1; NM_001021201.2.
DR AlphaFoldDB; O42904; -.
DR SMR; O42904; -.
DR BioGRID; 276583; 10.
DR IntAct; O42904; 1.
DR STRING; 4896.SPBC119.13c.1; -.
DR iPTMnet; O42904; -.
DR MaxQB; O42904; -.
DR PaxDb; O42904; -.
DR PRIDE; O42904; -.
DR EnsemblFungi; SPBC119.13c.1; SPBC119.13c.1:pep; SPBC119.13c.
DR GeneID; 2540045; -.
DR KEGG; spo:SPBC119.13c; -.
DR PomBase; SPBC119.13c; prp31.
DR VEuPathDB; FungiDB:SPBC119.13c; -.
DR eggNOG; KOG2574; Eukaryota.
DR HOGENOM; CLU_026337_2_0_1; -.
DR InParanoid; O42904; -.
DR OMA; IIGNGPM; -.
DR PhylomeDB; O42904; -.
DR PRO; PR:O42904; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0071011; C:precatalytic spliceosome; IBA:GO_Central.
DR GO; GO:0097526; C:spliceosomal tri-snRNP complex; IBA:GO_Central.
DR GO; GO:0005687; C:U4 snRNP; IBA:GO_Central.
DR GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; IDA:PomBase.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IMP:PomBase.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0000244; P:spliceosomal tri-snRNP complex assembly; ISS:PomBase.
DR Gene3D; 1.10.246.90; -; 1.
DR InterPro; IPR042239; Nop_C.
DR InterPro; IPR002687; Nop_dom.
DR InterPro; IPR036070; Nop_dom_sf.
DR InterPro; IPR012976; NOSIC.
DR InterPro; IPR027105; Prp31.
DR InterPro; IPR019175; Prp31_C.
DR PANTHER; PTHR13904; PTHR13904; 1.
DR Pfam; PF01798; Nop; 1.
DR Pfam; PF09785; Prp31_C; 1.
DR SMART; SM00931; NOSIC; 1.
DR SUPFAM; SSF89124; SSF89124; 1.
DR PROSITE; PS51358; NOP; 1.
PE 3: Inferred from homology;
KW Coiled coil; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW Ribonucleoprotein; RNA-binding; Spliceosome.
FT CHAIN 1..518
FT /note="Pre-mRNA-processing factor 31"
FT /id="PRO_0000058588"
FT DOMAIN 239..357
FT /note="Nop"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00690"
FT REGION 359..382
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 112..147
FT /evidence="ECO:0000250"
FT COILED 205..239
FT /evidence="ECO:0000250"
FT SITE 271
FT /note="Interaction with U4 snRNA"
FT /evidence="ECO:0000250"
SQ SEQUENCE 518 AA; 57739 MW; EA2CBCB489438E6E CRC64;
MSLADELLAD LDDIEETTES TITDELGPDA KKRRLELQLE EGNGISAELE NDLDITKISD
SAQKLPSEVA NKFNDNNENI YQLLNSTRLR DIIEGTEKYK GTEKQAITGN IEDDLEYHLI
VDSNSIAMEI DDEILRLHRL VKEWYHDRFP ELSSLVLNAF DYCKTVSSLL NDLDNSKTKL
SFLPSATVMV IATTATTTVG KPLPDEMIKN VKNCCEAIQQ LGEEKQKIIE YVQSRISVVA
PNLSAVVGST TAANLIGIAG GLTRLGKFPA CNLPALGKRR LTTIGINNPA VSGDYGFLYM
SEIVQKTPPD VRKQAIRMTA AKVALAARID SIHEYPDGSF GISARKEVER KIEKLLEPPS
QKPTVALPVP DDRPKRRRGG RRIRKMKEQY AVTELRRLQN RVAFGKEEAE VFNFDETEGL
GMLGQEGEGK IRAVSIDSRT KLRLPKARKA QLQSMAQKNP LAASGLQSSL SFTPIQGIEL
VNPLLQRQQK VEEANKWFRD GVFTQIKKDS NEPKNKFS
