PRP31_XENTR
ID PRP31_XENTR Reviewed; 498 AA.
AC Q6NVP6;
DT 21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=U4/U6 small nuclear ribonucleoprotein Prp31;
DE AltName: Full=Pre-mRNA-processing factor 31;
GN Name=prpf31;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in pre-mRNA splicing as component of the
CC spliceosome. Required for the assembly of the U4/U5/U6 tri-snRNP
CC complex, one of the building blocks of the spliceosome.
CC {ECO:0000250|UniProtKB:Q8WWY3}.
CC -!- SUBUNIT: Identified in the spliceosome B complex. Component of the
CC U4/U6-U5 tri-snRNP complex. Component of some MLL1/MLL complex.
CC {ECO:0000250|UniProtKB:Q8WWY3}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8WWY3}. Nucleus
CC speckle {ECO:0000250|UniProtKB:Q8WWY3}. Nucleus, Cajal body
CC {ECO:0000250|UniProtKB:Q8WWY3}. Note=Predominantly found in speckles
CC and in Cajal bodies. {ECO:0000250|UniProtKB:Q8WWY3}.
CC -!- DOMAIN: Interacts with the snRNP via the Nop domain.
CC {ECO:0000250|UniProtKB:Q8WWY3}.
CC -!- DOMAIN: The coiled coil domain is formed by two non-contiguous helices.
CC {ECO:0000250|UniProtKB:Q8WWY3}.
CC -!- SIMILARITY: Belongs to the PRP31 family. {ECO:0000305}.
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DR EMBL; BC067959; AAH67959.1; -; mRNA.
DR RefSeq; NP_998859.1; NM_213694.1.
DR RefSeq; XP_012821785.1; XM_012966331.2.
DR AlphaFoldDB; Q6NVP6; -.
DR SMR; Q6NVP6; -.
DR STRING; 8364.ENSXETP00000023852; -.
DR PaxDb; Q6NVP6; -.
DR DNASU; 407890; -.
DR Ensembl; ENSXETT00000023852; ENSXETP00000023852; ENSXETG00000010916.
DR GeneID; 407890; -.
DR KEGG; xtr:407890; -.
DR CTD; 26121; -.
DR Xenbase; XB-GENE-489336; prpf31.
DR eggNOG; KOG2574; Eukaryota.
DR HOGENOM; CLU_026337_2_0_1; -.
DR InParanoid; Q6NVP6; -.
DR OMA; LPKATIM; -.
DR OrthoDB; 791296at2759; -.
DR PhylomeDB; Q6NVP6; -.
DR TreeFam; TF300677; -.
DR Proteomes; UP000008143; Chromosome 7.
DR Proteomes; UP000790000; Unplaced.
DR Bgee; ENSXETG00000010916; Expressed in blastula and 12 other tissues.
DR ExpressionAtlas; Q6NVP6; baseline.
DR GO; GO:0015030; C:Cajal body; IEA:UniProtKB-SubCell.
DR GO; GO:0071339; C:MLL1 complex; ISS:UniProtKB.
DR GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0071011; C:precatalytic spliceosome; IBA:GO_Central.
DR GO; GO:0097526; C:spliceosomal tri-snRNP complex; IBA:GO_Central.
DR GO; GO:0071005; C:U2-type precatalytic spliceosome; ISS:UniProtKB.
DR GO; GO:0005687; C:U4 snRNP; IBA:GO_Central.
DR GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; ISS:UniProtKB.
DR GO; GO:0005690; C:U4atac snRNP; ISS:UniProtKB.
DR GO; GO:0030622; F:U4atac snRNA binding; ISS:UniProtKB.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0000244; P:spliceosomal tri-snRNP complex assembly; IEA:InterPro.
DR Gene3D; 1.10.246.90; -; 1.
DR InterPro; IPR042239; Nop_C.
DR InterPro; IPR002687; Nop_dom.
DR InterPro; IPR036070; Nop_dom_sf.
DR InterPro; IPR012976; NOSIC.
DR InterPro; IPR027105; Prp31.
DR InterPro; IPR019175; Prp31_C.
DR PANTHER; PTHR13904; PTHR13904; 1.
DR Pfam; PF01798; Nop; 1.
DR Pfam; PF09785; Prp31_C; 1.
DR SMART; SM00931; NOSIC; 1.
DR SUPFAM; SSF89124; SSF89124; 1.
DR PROSITE; PS51358; NOP; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW Ribonucleoprotein; RNA-binding; Spliceosome.
FT CHAIN 1..498
FT /note="U4/U6 small nuclear ribonucleoprotein Prp31"
FT /id="PRO_0000227803"
FT DOMAIN 214..332
FT /note="Nop"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00690"
FT REGION 333..356
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 84..119
FT /evidence="ECO:0000250|UniProtKB:Q8WWY3"
FT COILED 180..214
FT /evidence="ECO:0000250|UniProtKB:Q8WWY3"
FT MOTIF 350..363
FT /note="Nuclear localization signal (NLS)"
FT /evidence="ECO:0000250|UniProtKB:Q8WWY3"
FT SITE 246
FT /note="Interaction with U4 snRNA"
FT /evidence="ECO:0000250|UniProtKB:Q8WWY3"
FT SITE 269
FT /note="Interaction with U4 snRNA and U4atac snRNA"
FT /evidence="ECO:0000250|UniProtKB:Q8WWY3"
FT SITE 288
FT /note="Interaction with U4atac snRNA"
FT /evidence="ECO:0000250|UniProtKB:Q8WWY3"
FT SITE 292
FT /note="Interaction with U4 snRNA and U4atac snRNA"
FT /evidence="ECO:0000250|UniProtKB:Q8WWY3"
FT SITE 297
FT /note="Interaction with U4 snRNA and U4atac snRNA"
FT /evidence="ECO:0000250|UniProtKB:Q8WWY3"
SQ SEQUENCE 498 AA; 55505 MW; F27DC5F2CAD12F65 CRC64;
MSLADELLAD LEEAAEEEEE NLIDEDDLET IEEVQEEMQV DLNAESVKSI AKLSDSKLFS
EILLKIDGYI KKQPKASEVM GPVEAAPEYK VIVDANNLTV EIENELNIIH KFIRDKYSKR
FPELESLVPN ALDYIRTVKE LGNNLDKCKN NENLQQILTN ATIMVVSVTA STTQGQQLTD
EELERIEEAC DMALELNQSK HRIYEYVESR MSFIAPNLSI IVGASTAAKI MGIAGGLTNL
SKMPACNVML LGAQRKTLSG FSSTSVLPHT GYIYHSDIVQ SLPPDLHRKA ARLVSAKCTL
AARVDSFHES SEGKVGYDLK EEIERKFDKW QEPPPVKQVK PLPAPLDGQR KKRGGRRYRK
MKERLGLTEI RKQANRMSFA EIEEDAYQED LGFSLGHLGK SGSGRIRQAQ VNEATKARIS
KTLQRTLQKQ SVVYGGKSTI RDRSSGTASS VAFTPLQGLE IVNPQAAEKK VAEANQKYFS
SMAEFLKVKS EKSGTMTQ
