PRP38_ARATH
ID PRP38_ARATH Reviewed; 355 AA.
AC Q8LB54; Q0WQD7;
DT 09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 25-MAY-2022, entry version 101.
DE RecName: Full=Pre-mRNA-splicing factor 38;
GN Name=PRP38; OrderedLocusNames=At2g40650; ORFNames=T7D17.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INTERACTION WITH SR45A, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=19238562; DOI=10.1007/s11103-009-9469-y;
RA Tanabe N., Kimura A., Yoshimura K., Shigeoka S.;
RT "Plant-specific SR-related protein atSR45a interacts with spliceosomal
RT proteins in plant nucleus.";
RL Plant Mol. Biol. 70:241-252(2009).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-204, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- FUNCTION: Probably required for the first pre-mRNA cleavage reaction
CC catalyzed by the spliceosome. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with SR45A. {ECO:0000269|PubMed:19238562}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19238562}.
CC -!- SIMILARITY: Belongs to the PRP38 family. {ECO:0000305}.
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DR EMBL; CP002685; AEC09860.1; -; Genomic_DNA.
DR EMBL; AK228762; BAF00662.1; -; mRNA.
DR EMBL; AY087415; AAM64964.1; -; mRNA.
DR RefSeq; NP_565937.1; NM_129627.3.
DR AlphaFoldDB; Q8LB54; -.
DR SMR; Q8LB54; -.
DR BioGRID; 3998; 21.
DR STRING; 3702.AT2G40650.1; -.
DR iPTMnet; Q8LB54; -.
DR PaxDb; Q8LB54; -.
DR PRIDE; Q8LB54; -.
DR ProteomicsDB; 226378; -.
DR EnsemblPlants; AT2G40650.1; AT2G40650.1; AT2G40650.
DR GeneID; 818660; -.
DR Gramene; AT2G40650.1; AT2G40650.1; AT2G40650.
DR KEGG; ath:AT2G40650; -.
DR Araport; AT2G40650; -.
DR TAIR; locus:2064811; AT2G40650.
DR eggNOG; KOG2889; Eukaryota.
DR HOGENOM; CLU_039466_1_2_1; -.
DR InParanoid; Q8LB54; -.
DR OMA; FYMRLVG; -.
DR OrthoDB; 1182767at2759; -.
DR PhylomeDB; Q8LB54; -.
DR PRO; PR:Q8LB54; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8LB54; baseline and differential.
DR Genevisible; Q8LB54; AT.
DR GO; GO:0071011; C:precatalytic spliceosome; IBA:GO_Central.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR InterPro; IPR005037; PRP38.
DR InterPro; IPR024767; PRP38_C.
DR PANTHER; PTHR23142; PTHR23142; 1.
DR Pfam; PF03371; PRP38; 1.
DR Pfam; PF12871; PRP38_assoc; 1.
PE 1: Evidence at protein level;
KW mRNA processing; mRNA splicing; Nucleus; Phosphoprotein;
KW Reference proteome; Spliceosome.
FT CHAIN 1..355
FT /note="Pre-mRNA-splicing factor 38"
FT /id="PRO_0000429606"
FT REGION 182..355
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 185..203
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 204..289
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 301..339
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 204
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
FT CONFLICT 149
FT /note="E -> G (in Ref. 3; BAF00662)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 355 AA; 43172 MW; 3E844AFC225A59DB CRC64;
MANRTDPLAK NIRGTNPQNL VEKIVRTKIY QHTFWKEQCF GLTAETLVDK AMELDHLGGT
FGGSRKPTPF LCLILKMLQI QPEKEIVVEF IKNDDYKYVR ILGAFYLRLT GTDVDVYRYL
EPLYNDYRKV RQKLSDGKFS LTHVDEVIEE LLTKDYSCDI AMPRLKKRWT LEQNGLLEPR
KSVLEDDFEE EEEKEENEGI ADGSEDEMDQ RRKSPERERE RDRDRRRDSH RHRDRDYDRD
YDMDRDHDRD YERERGHGRD RDRERDRDHY RERDRDRERG RDRERDRRDR ARRRSRSRSR
DRKRHETDDV RDREEPKKKK EKKEKMKEDG TDHPNPEIAE MNRLRASLGM KPLRD
