PRP38_DROME
ID PRP38_DROME Reviewed; 330 AA.
AC Q7JVL3;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Pre-mRNA-splicing factor 38 {ECO:0000305};
GN Name=Prp38 {ECO:0000312|FlyBase:FBgn0050342};
GN ORFNames=CG30342 {ECO:0000312|FlyBase:FBgn0050342};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAM50703.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAM50703.1};
RC TISSUE=Ovary {ECO:0000312|EMBL:AAM50703.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION, INTERACTION WITH MFAP1, AND SUBCELLULAR LOCATION.
RX PubMed=18765666; DOI=10.1074/jbc.m803512200;
RA Andersen D.S., Tapon N.;
RT "Drosophila MFAP1 is required for pre-mRNA processing and G2/M
RT progression.";
RL J. Biol. Chem. 283:31256-31267(2008).
RN [5] {ECO:0000305}
RP IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, IDENTIFICATION IN THE
RP SPLICEOSOME C COMPLEX, AND SUBCELLULAR LOCATION.
RX PubMed=18981222; DOI=10.1128/mcb.01415-08;
RA Herold N., Will C.L., Wolf E., Kastner B., Urlaub H., Luhrmann R.;
RT "Conservation of the protein composition and electron microscopy structure
RT of Drosophila melanogaster and human spliceosomal complexes.";
RL Mol. Cell. Biol. 29:281-301(2009).
RN [6]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=24244416; DOI=10.1371/journal.pone.0079048;
RA Klusza S., Novak A., Figueroa S., Palmer W., Deng W.M.;
RT "Prp22 and spliceosome components regulate chromatin dynamics in germ-line
RT polyploid cells.";
RL PLoS ONE 8:E79048-E79048(2013).
CC -!- FUNCTION: Required for pre-mRNA splicing. {ECO:0000269|PubMed:18765666,
CC ECO:0000305|PubMed:18981222}.
CC -!- SUBUNIT: Component of the spliceosome C complex (PubMed:18981222).
CC Interacts with Mfap1 (via C-terminus) (PubMed:18765666).
CC {ECO:0000269|PubMed:18765666, ECO:0000269|PubMed:18981222}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:18765666,
CC ECO:0000269|PubMed:18981222, ECO:0000269|PubMed:24244416}.
CC -!- TISSUE SPECIFICITY: Detected in all germal and follicle cells.
CC {ECO:0000269|PubMed:24244416}.
CC -!- SIMILARITY: Belongs to the PRP38 family. {ECO:0000305}.
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DR EMBL; AE013599; AAF58976.3; -; Genomic_DNA.
DR EMBL; AY118843; AAM50703.1; -; mRNA.
DR RefSeq; NP_610463.2; NM_136619.3.
DR AlphaFoldDB; Q7JVL3; -.
DR SMR; Q7JVL3; -.
DR IntAct; Q7JVL3; 1.
DR STRING; 7227.FBpp0087663; -.
DR PaxDb; Q7JVL3; -.
DR PRIDE; Q7JVL3; -.
DR DNASU; 35934; -.
DR EnsemblMetazoa; FBtr0088582; FBpp0087663; FBgn0050342.
DR GeneID; 35934; -.
DR KEGG; dme:Dmel_CG30342; -.
DR UCSC; CG30342-RA; d. melanogaster.
DR CTD; 35934; -.
DR FlyBase; FBgn0050342; Prp38.
DR VEuPathDB; VectorBase:FBgn0050342; -.
DR eggNOG; KOG2889; Eukaryota.
DR GeneTree; ENSGT00730000111085; -.
DR HOGENOM; CLU_039466_1_0_1; -.
DR InParanoid; Q7JVL3; -.
DR OMA; FYMRLVG; -.
DR OrthoDB; 1182767at2759; -.
DR PhylomeDB; Q7JVL3; -.
DR Reactome; R-DME-72163; mRNA Splicing - Major Pathway.
DR BioGRID-ORCS; 35934; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 35934; -.
DR PRO; PR:Q7JVL3; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0050342; Expressed in eye disc (Drosophila) and 24 other tissues.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; HDA:FlyBase.
DR GO; GO:0071011; C:precatalytic spliceosome; HDA:FlyBase.
DR GO; GO:0032991; C:protein-containing complex; IPI:FlyBase.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IMP:FlyBase.
DR GO; GO:0010389; P:regulation of G2/M transition of mitotic cell cycle; IMP:FlyBase.
DR InterPro; IPR005037; PRP38.
DR InterPro; IPR024767; PRP38_C.
DR PANTHER; PTHR23142; PTHR23142; 1.
DR Pfam; PF03371; PRP38; 1.
DR Pfam; PF12871; PRP38_assoc; 1.
PE 1: Evidence at protein level;
KW mRNA processing; mRNA splicing; Nucleus; Reference proteome; Spliceosome.
FT CHAIN 1..330
FT /note="Pre-mRNA-splicing factor 38"
FT /id="PRO_0000438524"
FT REGION 182..330
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 197..214
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 227..330
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 330 AA; 40088 MW; 6AC4FAD5AF5A7C49 CRC64;
MANRTVKEAK NVHGTNPQYL IEKIIRSRIY DSKYWKEQCF ALTAELLVDK AMELRFVGGV
YGGNIKPTQF LCLTLKMLQI QPEKDIVVEF IKNEEFKYVR ALGAFYLRLT GAALDCYKYL
EPLYIDNRKL RRQNRAGQFE IVYMDEYIDE LLRNDRVCDI ILPRIQKRSI LEENNEIEPK
VSVLDEDLDD ELPSDEEKAD ETNRPKENST AVRRPRRVRS KSRSRSRERE RRSGQGNSAR
SRDYYDELED YDRQRNRVRN RDTHNEDYDR RQNNGRHDRE RERQDRDSIR ERERDGDRDR
RDRERERERD RGRHDQRERD SRGERDRRRY
