PRP38_YEAST
ID PRP38_YEAST Reviewed; 242 AA.
AC Q00723; D6VUK7;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1993, sequence version 1.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Pre-mRNA-splicing factor 38;
DE AltName: Full=Pre-mRNA-processing factor 38;
GN Name=PRP38; OrderedLocusNames=YGR075C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=1508195; DOI=10.1128/mcb.12.9.3939-3947.1992;
RA Blanton S., Srinivasan A., Rymond B.C.;
RT "PRP38 encodes a yeast protein required for pre-mRNA splicing and
RT maintenance of stable U6 small nuclear RNA levels.";
RL Mol. Cell. Biol. 12:3939-3947(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP FUNCTION, INTERACTION WITH U4/U6.U5 TRI-SNRNP COMPLEX, AND MUTAGENESIS OF
RP CYS-87.
RX PubMed=9582287; DOI=10.1093/emboj/17.10.2938;
RA Xie J., Beickman K., Otte E., Rymond B.C.;
RT "Progression through the spliceosome cycle requires Prp38p function for
RT U4/U6 snRNA dissociation.";
RL EMBO J. 17:2938-2946(1998).
RN [6]
RP SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=10449419; DOI=10.1093/emboj/18.16.4535;
RA Gottschalk A., Neubauer G., Banroques J., Mann M., Luehrmann R.,
RA Fabrizio P.;
RT "Identification by mass spectrometry and functional analysis of novel
RT proteins of the yeast [U4/U6.U5] tri-snRNP.";
RL EMBO J. 18:4535-4548(1999).
RN [7]
RP IDENTIFICATION IN U4/U6.U5 TRI-SNRNP COMPLEX BY MASS SPECTROMETRY.
RX PubMed=10377396; DOI=10.1073/pnas.96.13.7226;
RA Stevens S.W., Abelson J.;
RT "Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein
RT particle and identification of its proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:7226-7231(1999).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=11804584; DOI=10.1016/s1097-2765(02)00436-7;
RA Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D.,
RA Abelson J.;
RT "Composition and functional characterization of the yeast spliceosomal
RT penta-snRNP.";
RL Mol. Cell 9:31-44(2002).
RN [9]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [10]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Required for pre-mRNA splicing and maintenance of stable U6
CC small nuclear RNA levels. Implicated in the formation of stable and
CC biologically active snRNP structures. As part of the U4/U6.U5 tri-snRNP
CC particle, dispensible for spliceosome assembly, but required for
CC conformational changes, which result in U4 snRNA release and the
CC subsequent catalytic activation of the spliceosome.
CC {ECO:0000269|PubMed:1508195, ECO:0000269|PubMed:9582287}.
CC -!- SUBUNIT: Component of the U4/U6-U5 tri-snRNP complex composed of the
CC U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31,
CC PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3,
CC SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1.
CC {ECO:0000269|PubMed:10377396, ECO:0000269|PubMed:10449419}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 2380 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the PRP38 family. {ECO:0000305}.
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DR EMBL; L04669; AAA35054.1; -; Genomic_DNA.
DR EMBL; M95921; AAA34913.1; -; Genomic_DNA.
DR EMBL; Z72858; CAA97077.1; -; Genomic_DNA.
DR EMBL; AY558507; AAS56833.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA08168.1; -; Genomic_DNA.
DR PIR; S30888; S30888.
DR RefSeq; NP_011589.3; NM_001181204.3.
DR PDB; 5NRL; EM; 7.20 A; M=1-242.
DR PDB; 5ZWO; EM; 3.90 A; 0=1-242.
DR PDBsum; 5NRL; -.
DR PDBsum; 5ZWO; -.
DR AlphaFoldDB; Q00723; -.
DR SMR; Q00723; -.
DR BioGRID; 33317; 127.
DR ComplexPortal; CPX-25; U4/U6.U5 tri-small nuclear ribonucleoprotein complex.
DR DIP; DIP-1648N; -.
DR IntAct; Q00723; 9.
DR MINT; Q00723; -.
DR STRING; 4932.YGR075C; -.
DR MaxQB; Q00723; -.
DR PaxDb; Q00723; -.
DR PRIDE; Q00723; -.
DR EnsemblFungi; YGR075C_mRNA; YGR075C; YGR075C.
DR GeneID; 852966; -.
DR KEGG; sce:YGR075C; -.
DR SGD; S000003307; PRP38.
DR VEuPathDB; FungiDB:YGR075C; -.
DR eggNOG; ENOG502RXT7; Eukaryota.
DR HOGENOM; CLU_1147968_0_0_1; -.
DR InParanoid; Q00723; -.
DR OMA; FKCLLMK; -.
DR BioCyc; YEAST:G3O-30787-MON; -.
DR PRO; PR:Q00723; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; Q00723; protein.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0071011; C:precatalytic spliceosome; IBA:GO_Central.
DR GO; GO:0005681; C:spliceosomal complex; IC:ComplexPortal.
DR GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; IDA:SGD.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IC:ComplexPortal.
DR GO; GO:0000388; P:spliceosome conformational change to release U4 (or U4atac) and U1 (or U11); IMP:SGD.
DR InterPro; IPR005037; PRP38.
DR PANTHER; PTHR23142; PTHR23142; 1.
DR Pfam; PF03371; PRP38; 1.
PE 1: Evidence at protein level;
KW 3D-structure; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW Ribonucleoprotein; Spliceosome.
FT CHAIN 1..242
FT /note="Pre-mRNA-splicing factor 38"
FT /id="PRO_0000058590"
FT REGION 217..242
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 224..242
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 87
FT /note="C->Y: In PRP83-2; temperature-sensitive; blocks
FT splicing of RPS17A after a 2 hour shift to the restrictive
FT temperature of 37 degrees Celsius; spliceosome assembly
FT arrested at the complex I stage."
FT /evidence="ECO:0000269|PubMed:9582287"
SQ SEQUENCE 242 AA; 27967 MW; 62EB225947AF4349 CRC64;
MAVNEFQVES NISPKQLNNQ SVSLVIPRLT RDKIHNSMYY KVNLSNESLR GNTMVELLKV
MIGAFGTIKG QNGHLHMMVL GGIEFKCILM KLIEIRPNFQ QLNFLLNVKN ENGFDSKYII
ALLLVYARLQ YYYLNGNNKN DDDENDLIKL FKVQLYKYSQ HYFKLKSFPL QVDCFAHSYN
EELCIIHIDE LVDWLATQDH IWGIPLGKCQ WNKIYNSDEE SSSSESESNG DSEDDNDTSS
ES
