PRP39_DROME
ID PRP39_DROME Reviewed; 1066 AA.
AC Q7KRW8; A8JRF2; A8JRF3; A8JRF4; Q86B75; Q9VAT3;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Pre-mRNA-processing factor 39;
DE AltName: Full=PRP39 homolog;
GN ORFNames=CG1646;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-252, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=17372656; DOI=10.1039/b617545g;
RA Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A.,
RA Eng J.K., Aebersold R., Tao W.A.;
RT "An integrated chemical, mass spectrometric and computational strategy for
RT (quantitative) phosphoproteomics: application to Drosophila melanogaster
RT Kc167 cells.";
RL Mol. Biosyst. 3:275-286(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-122; SER-148; SER-158;
RP SER-308 AND SER-313, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Involved in pre-mRNA splicing. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=B;
CC IsoId=Q7KRW8-1; Sequence=Displayed;
CC Name=A; Synonyms=C, E;
CC IsoId=Q7KRW8-3; Sequence=VSP_037166;
CC Name=H;
CC IsoId=Q7KRW8-6; Sequence=VSP_037168, VSP_037169;
CC -!- SIMILARITY: Belongs to the PRP39 family. {ECO:0000305}.
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DR EMBL; AE014297; ABW08794.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF56816.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN14302.2; -; Genomic_DNA.
DR EMBL; AE014297; AAO41607.1; -; Genomic_DNA.
DR EMBL; AE014297; ABW08793.1; -; Genomic_DNA.
DR EMBL; AY051737; AAK93161.1; -; mRNA.
DR RefSeq; NP_001097957.1; NM_001104487.2. [Q7KRW8-3]
DR RefSeq; NP_001097958.1; NM_001104488.3. [Q7KRW8-6]
DR RefSeq; NP_651634.1; NM_143377.4. [Q7KRW8-3]
DR RefSeq; NP_733256.2; NM_170377.3. [Q7KRW8-1]
DR RefSeq; NP_788753.1; NM_176576.3. [Q7KRW8-3]
DR AlphaFoldDB; Q7KRW8; -.
DR SMR; Q7KRW8; -.
DR BioGRID; 68275; 5.
DR IntAct; Q7KRW8; 2.
DR STRING; 7227.FBpp0084691; -.
DR iPTMnet; Q7KRW8; -.
DR PaxDb; Q7KRW8; -.
DR PRIDE; Q7KRW8; -.
DR DNASU; 43399; -.
DR EnsemblMetazoa; FBtr0085322; FBpp0084691; FBgn0039600. [Q7KRW8-1]
DR EnsemblMetazoa; FBtr0085323; FBpp0084692; FBgn0039600. [Q7KRW8-3]
DR EnsemblMetazoa; FBtr0085325; FBpp0084694; FBgn0039600. [Q7KRW8-3]
DR EnsemblMetazoa; FBtr0113299; FBpp0112211; FBgn0039600. [Q7KRW8-3]
DR EnsemblMetazoa; FBtr0113301; FBpp0112213; FBgn0039600. [Q7KRW8-6]
DR GeneID; 43399; -.
DR KEGG; dme:Dmel_CG1646; -.
DR UCSC; CG1646-RA; d. melanogaster.
DR UCSC; CG1646-RB; d. melanogaster. [Q7KRW8-1]
DR UCSC; CG1646-RD; d. melanogaster.
DR UCSC; CG1646-RH; d. melanogaster.
DR FlyBase; FBgn0039600; CG1646.
DR VEuPathDB; VectorBase:FBgn0039600; -.
DR eggNOG; KOG1258; Eukaryota.
DR GeneTree; ENSGT00940000168631; -.
DR InParanoid; Q7KRW8; -.
DR OMA; REAYTKF; -.
DR PhylomeDB; Q7KRW8; -.
DR BioGRID-ORCS; 43399; 0 hits in 1 CRISPR screen.
DR ChiTaRS; CG1646; fly.
DR GenomeRNAi; 43399; -.
DR PRO; PR:Q7KRW8; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0039600; Expressed in eye disc (Drosophila) and 26 other tissues.
DR ExpressionAtlas; Q7KRW8; baseline and differential.
DR Genevisible; Q7KRW8; DM.
DR GO; GO:0000243; C:commitment complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IC:FlyBase.
DR GO; GO:0005685; C:U1 snRNP; ISS:FlyBase.
DR GO; GO:0071004; C:U2-type prespliceosome; IBA:GO_Central.
DR GO; GO:0000395; P:mRNA 5'-splice site recognition; IBA:GO_Central.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:FlyBase.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; IMP:FlyBase.
DR Gene3D; 1.25.40.10; -; 2.
DR InterPro; IPR003107; HAT.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR SMART; SM00386; HAT; 6.
DR SUPFAM; SSF48452; SSF48452; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; mRNA processing; mRNA splicing; Nucleus;
KW Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..1066
FT /note="Pre-mRNA-processing factor 39"
FT /id="PRO_0000372647"
FT REPEAT 393..425
FT /note="HAT 1"
FT REPEAT 427..459
FT /note="HAT 2"
FT REPEAT 463..498
FT /note="HAT 3"
FT REPEAT 685..717
FT /note="HAT 4"
FT REPEAT 719..751
FT /note="HAT 5"
FT REPEAT 757..793
FT /note="HAT 6"
FT REPEAT 1029..1066
FT /note="HAT 7"
FT REGION 1..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 145..358
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 552..643
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 951..985
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 174..190
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 270..299
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 300..316
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 317..358
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 552..606
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 621..643
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 968..982
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 122
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 148
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 158
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 252
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:17372656"
FT MOD_RES 308
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 313
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT VAR_SEQ 1..57
FT /note="Missing (in isoform A)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_037166"
FT VAR_SEQ 436..501
FT /note="FERGLEAIPLSVDLWIHYLMHVKSNHGDDETFVRSQYERAVKACGLEFRSDK
FT LWDAYIRWENESKR -> STPLTFGRANKYLSQFLVLRLQLRLQLIFIYLLLNAIYYDL
FT IKILHSINATSTKIHQIEKTKPNLP (in isoform H)"
FT /evidence="ECO:0000305"
FT /id="VSP_037168"
FT VAR_SEQ 502..1066
FT /note="Missing (in isoform H)"
FT /evidence="ECO:0000305"
FT /id="VSP_037169"
SQ SEQUENCE 1066 AA; 120546 MW; D73DB122BE290530 CRC64;
MASEGENVVM ESPGRRTRSG RKAASPAASL PTRSTRRTSK RNVQLSDHEE EAEEQVVMVQ
EEEEPFRGTN TAEAPALEPQ FEETQHQIED DVEMLPAEAA GVDEKSSSFP FGTVAELHSE
TSDDIKETRG QAGVDTLLAS LAGDNANSLP SVGGNDESDN VKKADFEYNV DSLQPQMEKM
NSSEDSSSQH AIAEDLADAD TSNATIVNTE IVSEDELPLP SKPEINDAEE VSDEELPAPQ
RAELPEDAEV ISEDELPASN NNNTATEPKA PLKRKAENES VKSTEDKDAS SHESSTKDKS
SEQYNPGSPT SESNDAQPSE KKLKVEESEP KEKKKEKERD KDKEKDKDKD NNKDKEKERK
KLPDLDKYWR AVKEDSTDFT GWTYLLQYVD NESDAEAARE AYDTFLSHYP YCYGYWRKYA
DYEKRKGIKA NCYKVFERGL EAIPLSVDLW IHYLMHVKSN HGDDETFVRS QYERAVKACG
LEFRSDKLWD AYIRWENESK RYHRVVQIYD RLLAIPTQGY NGHFDNFQDL INQHDVTITL
ANEEVIRLRK DFHERQQSKS SKSSSKHRRD SSSSSKDKDS KEREREKDKD KDKDKDKEKR
ETVGGGVGKS PKDTSETQVD ESDSTTDLTT ESESSHAASK PAMQIDFSDL STLNDEEVVS
IRDRAISARR KVHKLTVSAV TARWSFEEGI KRPYFHVKPL ERAQLKNWKD YLDFEIEKGD
RERVLVLFER CLIACALYDE FWLKMLRYLE SLEDQSGVVD LVRDVYRRAC RIHHPDKPSL
HLMWAAFEEC QMNFDDAAEI LQRIDQRCPN LLQLSYRRIN VERRRGALDK CRELYKHYIE
STKNKGIAGS LAIKYARFLN KICHDLDAGL AALQQALERD PANTRVALQM IDLCLQRPKV
DEQEVVEIMD KFMARADIEP DQKVLFAQRK VEFLEDFGST ARGLQDAQRA LQQALTKAKE
AQKKSDGSPS RKNSSSSKEG PVPTGSAAAA YNNGGSAAAV AGYNYGAANP YYGQQNTAAA
YPSQTPQQAS YDSYYNQWGY GSGGASANSG GSGYNYGQWS GYGNYY
