PRP3_SCHPO
ID PRP3_SCHPO Reviewed; 542 AA.
AC Q09856;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=U4/U5/U6 small nuclear ribonucleoprotein prp3;
DE AltName: Full=Pre-mRNA-splicing factor 3;
GN Name=prp3; ORFNames=SPAC29E6.02, SPAC30.06;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE U4/U5/U6
RP TRI-SNRNP COMPLEX.
RX PubMed=15755920; DOI=10.1128/ec.4.3.577-587.2005;
RA Carnahan R.H., Feoktistova A., Ren L., Niessen S., Yates J.R. III,
RA Gould K.L.;
RT "Dim1p is required for efficient splicing and export of mRNA encoding
RT lid1p, a component of the fission yeast anaphase-promoting complex.";
RL Eukaryot. Cell 4:577-587(2005).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE U4/U5/U6
RP TRI-SNRNP COMPLEX.
RX PubMed=21386897; DOI=10.1371/journal.pone.0016719;
RA Ren L., McLean J.R., Hazbun T.R., Fields S., Vander Kooi C., Ohi M.D.,
RA Gould K.L.;
RT "Systematic two-hybrid and comparative proteomic analyses reveal novel
RT yeast pre-mRNA splicing factors connected to Prp19.";
RL PLoS ONE 6:E16719-E16719(2011).
RN [5]
RP FUNCTION.
RX PubMed=22240020; DOI=10.1016/j.bbrc.2011.12.128;
RA Watanabe N., Ikeda T., Mizuki F., Tani T.;
RT "Characterization of the ptr5+ gene involved in nuclear mRNA export in
RT fission yeast.";
RL Biochem. Biophys. Res. Commun. 418:62-66(2012).
CC -!- FUNCTION: Participates in pre-mRNA splicing and in nuclear mRNA export.
CC May play a role in the assembly of the U4/U5/U6 tri-snRNP complex.
CC {ECO:0000269|PubMed:22240020}.
CC -!- SUBUNIT: Component of the U4/U5/U6 tri-snRNP complex.
CC {ECO:0000269|PubMed:15755920, ECO:0000269|PubMed:21386897}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16823372}. Cytoplasm,
CC cytoskeleton, spindle {ECO:0000269|PubMed:16823372}. Cytoplasm,
CC cytoskeleton, spindle pole {ECO:0000269|PubMed:16823372}.
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DR EMBL; Z66525; CAA91424.1; -; Genomic_DNA.
DR EMBL; CU329670; CAB66465.1; -; Genomic_DNA.
DR PIR; S62508; S62508.
DR RefSeq; NP_594560.1; NM_001019989.2.
DR AlphaFoldDB; Q09856; -.
DR SMR; Q09856; -.
DR BioGRID; 278182; 9.
DR STRING; 4896.SPAC29E6.02.1; -.
DR iPTMnet; Q09856; -.
DR MaxQB; Q09856; -.
DR PaxDb; Q09856; -.
DR PRIDE; Q09856; -.
DR EnsemblFungi; SPAC29E6.02.1; SPAC29E6.02.1:pep; SPAC29E6.02.
DR GeneID; 2541686; -.
DR KEGG; spo:SPAC29E6.02; -.
DR PomBase; SPAC29E6.02; prp3.
DR VEuPathDB; FungiDB:SPAC29E6.02; -.
DR eggNOG; KOG2769; Eukaryota.
DR HOGENOM; CLU_015750_2_2_1; -.
DR InParanoid; Q09856; -.
DR OMA; CVMHPRF; -.
DR PhylomeDB; Q09856; -.
DR PRO; PR:Q09856; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0072686; C:mitotic spindle; HDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
DR GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
DR GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; IDA:PomBase.
DR GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IMP:PomBase.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR InterPro; IPR013881; Pre-mRNA_splic_Prp3.
DR InterPro; IPR027104; Prp3.
DR InterPro; IPR010541; Prp3_C.
DR PANTHER; PTHR14212; PTHR14212; 1.
DR Pfam; PF06544; DUF1115; 1.
DR Pfam; PF08572; PRP3; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; mRNA processing; mRNA splicing; mRNA transport;
KW Nucleus; Reference proteome; Spliceosome; Transport.
FT CHAIN 1..542
FT /note="U4/U5/U6 small nuclear ribonucleoprotein prp3"
FT /id="PRO_0000116420"
FT REGION 1..32
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 151..191
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 290..329
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 367..388
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 168..186
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 297..323
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 542 AA; 62589 MW; 91093B7F59C5B32B CRC64;
MDRNKRRLES SQLRESSSPN SQNKPNAMEE IKRRRLQLEQ RLAQQAQVPW EKRSENGNNA
GMETIQNRIS ELKEKTAKRF NANIPKSEGL FRDDSNGAKG GLKVGIHPVL LDGNIQNTIL
TPENRKRTAS FSTKGVSLSQ HQLLKPPAIT EQNPFLDTAP TPRLEDSPFY DPRTKESRKT
RGSRNLHLNE SGKFIEEANQ ARRQARLEDL KKRIALHSHK AGIEDELDIT SKSIGRDTIP
NIEWWDLPFI KDYNDYGDEN NWLIDGPQSI INSAIQHPIP VLPPYAKNQP SSHSVFLTKK
EQKKIRRQTR AEARKEKQDR QLLGIEPPEP PKVKLSNLMH VLGDDAIKDP TKIEAEVRKQ
VEERRLRHER ENEERKLTPE ERKEKAFRKK DEDSAAGLRC LVFRIKYLAH RPHRLKIDLN
AKQWGATGVC ILNANFNLVI FEAGQKAIKK LKRLMLERID WTDTSRNSII AQGNKLVDTE
GRELNYTENT CNLVWEGEIG RRAFRYWSFR SCPSENDAKS YLEEQGGAEH FWMLAKSWSE
NV
