PRP3_YEAST
ID PRP3_YEAST Reviewed; 469 AA.
AC Q03338; D6VT97;
DT 26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=U4/U6 small nuclear ribonucleoprotein PRP3;
DE AltName: Full=Pre-mRNA-splicing factor 3;
GN Name=PRP3; Synonyms=RNA3; OrderedLocusNames=YDR473C; ORFNames=D8035.16;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION.
RX PubMed=9326489;
RA Anthony J.G., Weidenhammer E.M., Woolford J.L. Jr.;
RT "The yeast Prp3 protein is a U4/U6 snRNP protein necessary for integrity of
RT the U4/U6 snRNP and the U4/U6.U5 tri-snRNP.";
RL RNA 3:1143-1152(1997).
RN [4]
RP SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=10449419; DOI=10.1093/emboj/18.16.4535;
RA Gottschalk A., Neubauer G., Banroques J., Mann M., Luehrmann R.,
RA Fabrizio P.;
RT "Identification by mass spectrometry and functional analysis of novel
RT proteins of the yeast [U4/U6.U5] tri-snRNP.";
RL EMBO J. 18:4535-4548(1999).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [7]
RP SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, AND ELECTRON
RP MICROSCOPY.
RX PubMed=18953335; DOI=10.1038/nsmb.1506;
RA Hacker I., Sander B., Golas M.M., Wolf E., Karagoz E., Kastner B.,
RA Stark H., Fabrizio P., Luhrmann R.;
RT "Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-
RT snRNP by electron microscopy.";
RL Nat. Struct. Mol. Biol. 15:1206-1212(2008).
CC -!- FUNCTION: Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-
CC snRNP complex, one of the building blocks of the spliceosome.
CC {ECO:0000269|PubMed:9326489}.
CC -!- SUBUNIT: Component of the U4/U6-U5 tri-snRNP complex composed of the
CC U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31,
CC PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3,
CC SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1.
CC {ECO:0000269|PubMed:10449419, ECO:0000269|PubMed:18953335}.
CC -!- INTERACTION:
CC Q03338; P20053: PRP4; NbExp=4; IntAct=EBI-421, EBI-219;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- MISCELLANEOUS: Present with 432 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; U33050; AAB64932.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA12307.1; -; Genomic_DNA.
DR PIR; S69640; S69640.
DR RefSeq; NP_010761.3; NM_001180781.3.
DR PDB; 3JCM; EM; 3.80 A; K=1-469.
DR PDB; 4YHU; X-ray; 2.70 A; A/B/C=296-469.
DR PDB; 4YHV; X-ray; 2.00 A; A=325-469.
DR PDB; 4YHW; X-ray; 3.25 A; A/B=296-469.
DR PDB; 5GAN; EM; 3.60 A; G=1-469.
DR PDB; 5GAP; EM; 3.60 A; G=1-469.
DR PDB; 5NRL; EM; 7.20 A; G=1-469.
DR PDB; 5ZWM; EM; 3.40 A; J=1-469.
DR PDB; 5ZWO; EM; 3.90 A; J=1-469.
DR PDBsum; 3JCM; -.
DR PDBsum; 4YHU; -.
DR PDBsum; 4YHV; -.
DR PDBsum; 4YHW; -.
DR PDBsum; 5GAN; -.
DR PDBsum; 5GAP; -.
DR PDBsum; 5NRL; -.
DR PDBsum; 5ZWM; -.
DR PDBsum; 5ZWO; -.
DR AlphaFoldDB; Q03338; -.
DR SMR; Q03338; -.
DR BioGRID; 32526; 188.
DR ComplexPortal; CPX-25; U4/U6.U5 tri-small nuclear ribonucleoprotein complex.
DR ComplexPortal; CPX-31; U4 small nuclear ribonucleoprotein complex.
DR ComplexPortal; CPX-32; U4/U6 small nuclear ribonucleoprotein complex.
DR DIP; DIP-5266N; -.
DR IntAct; Q03338; 29.
DR MINT; Q03338; -.
DR STRING; 4932.YDR473C; -.
DR iPTMnet; Q03338; -.
DR MaxQB; Q03338; -.
DR PaxDb; Q03338; -.
DR PRIDE; Q03338; -.
DR EnsemblFungi; YDR473C_mRNA; YDR473C; YDR473C.
DR GeneID; 852084; -.
DR KEGG; sce:YDR473C; -.
DR SGD; S000002881; PRP3.
DR VEuPathDB; FungiDB:YDR473C; -.
DR eggNOG; KOG2769; Eukaryota.
DR GeneTree; ENSGT00390000011497; -.
DR HOGENOM; CLU_015750_2_0_1; -.
DR InParanoid; Q03338; -.
DR OMA; CVMHPRF; -.
DR BioCyc; YEAST:G3O-30000-MON; -.
DR PRO; PR:Q03338; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q03338; protein.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0005681; C:spliceosomal complex; IC:ComplexPortal.
DR GO; GO:0005687; C:U4 snRNP; IC:ComplexPortal.
DR GO; GO:0071001; C:U4/U6 snRNP; IC:ComplexPortal.
DR GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; IDA:SGD.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0000245; P:spliceosomal complex assembly; IC:ComplexPortal.
DR GO; GO:0000387; P:spliceosomal snRNP assembly; IMP:SGD.
DR InterPro; IPR013881; Pre-mRNA_splic_Prp3.
DR InterPro; IPR027104; Prp3.
DR InterPro; IPR010541; Prp3_C.
DR PANTHER; PTHR14212; PTHR14212; 1.
DR Pfam; PF06544; DUF1115; 1.
DR Pfam; PF08572; PRP3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW Spliceosome.
FT CHAIN 1..469
FT /note="U4/U6 small nuclear ribonucleoprotein PRP3"
FT /id="PRO_0000097037"
FT REGION 1..45
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 26..42
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT STRAND 337..345
FT /evidence="ECO:0007829|PDB:4YHV"
FT HELIX 350..363
FT /evidence="ECO:0007829|PDB:4YHV"
FT STRAND 366..373
FT /evidence="ECO:0007829|PDB:4YHV"
FT STRAND 378..384
FT /evidence="ECO:0007829|PDB:4YHV"
FT HELIX 386..398
FT /evidence="ECO:0007829|PDB:4YHV"
FT STRAND 406..409
FT /evidence="ECO:0007829|PDB:4YHV"
FT STRAND 414..417
FT /evidence="ECO:0007829|PDB:4YHV"
FT STRAND 424..433
FT /evidence="ECO:0007829|PDB:4YHV"
FT STRAND 439..447
FT /evidence="ECO:0007829|PDB:4YHV"
FT HELIX 448..457
FT /evidence="ECO:0007829|PDB:4YHV"
FT HELIX 461..463
FT /evidence="ECO:0007829|PDB:4YHV"
SQ SEQUENCE 469 AA; 55870 MW; E977FD9F708BDD91 CRC64;
MPPRNTYEKG NPKRQNSPYY KPSFLRREET TNDEEKFQGH GLKTELHSAL KSSNLNLIRR
TYQTGENPYL SDPHDRGSSS RFNRRYERGL KFYQKGEISK RIAQERTLQK QQEEEELKRK
LKQEEDEKDK RKLIESGDLP NLELHEDKFL LDLSKFKIYY DNNHGYEWWD TAYLDEKGEL
MEKYDMNGTS PAEEKLAEDI DEVDDDDDDE HPSIRYVAHP LPEKINEAKV SIKAYLTQHE
RKRLRRNRRK MAREAREIKI KLGLLPKPEP KVKLSNMMSV FENDQNITDP TAWEKVVKDQ
VDLRKRKHLE ENERRHEDAI KRRKEAVNMN VEKPTVYHCK VFQFKNLQNP KIRFKLKMNS
KELSLKGLCL RIRDDGPGII IVVGNEKSCK FYENLVMKRI KWNEDFELHT NTGDIKMDMH
NNSISKTWEG YLQDCKFKGW FMKVCNDQDS LLRTLGQFDS EHFYSPVQT
