ID   PRP40_SCHPO             Reviewed;         695 AA.
AC   O14176;
DT   16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 123.
DE   RecName: Full=Pre-mRNA-processing protein prp40;
GN   Name=prp40; ORFNames=SPAC4D7.13;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-688 AND SER-689, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=18257517; DOI=10.1021/pr7006335;
RA   Wilson-Grady J.T., Villen J., Gygi S.P.;
RT   "Phosphoproteome analysis of fission yeast.";
RL   J. Proteome Res. 7:1088-1097(2008).
CC   -!- FUNCTION: Required for pre-spliceosome formation, which is the first
CC       step of pre-mRNA splicing. This protein is associated with snRNP U1 (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
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DR   EMBL; CU329670; CAB11285.1; -; Genomic_DNA.
DR   PIR; T38804; T38804.
DR   RefSeq; NP_594966.1; NM_001020397.2.
DR   AlphaFoldDB; O14176; -.
DR   SMR; O14176; -.
DR   BioGRID; 279831; 29.
DR   STRING; 4896.SPAC4D7.13.1; -.
DR   iPTMnet; O14176; -.
DR   MaxQB; O14176; -.
DR   PaxDb; O14176; -.
DR   PRIDE; O14176; -.
DR   EnsemblFungi; SPAC4D7.13.1; SPAC4D7.13.1:pep; SPAC4D7.13.
DR   GeneID; 2543409; -.
DR   KEGG; spo:SPAC4D7.13; -.
DR   PomBase; SPAC4D7.13; -.
DR   VEuPathDB; FungiDB:SPAC4D7.13; -.
DR   eggNOG; KOG0152; Eukaryota.
DR   HOGENOM; CLU_005825_1_1_1; -.
DR   InParanoid; O14176; -.
DR   OMA; HNVTTKE; -.
DR   PhylomeDB; O14176; -.
DR   PRO; PR:O14176; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0000243; C:commitment complex; ISS:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0005685; C:U1 snRNP; IDA:PomBase.
DR   GO; GO:0071004; C:U2-type prespliceosome; ISO:PomBase.
DR   GO; GO:0003723; F:RNA binding; ISO:PomBase.
DR   GO; GO:0000395; P:mRNA 5'-splice site recognition; IC:PomBase.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR   CDD; cd00201; WW; 2.
DR   Gene3D; 1.10.10.440; -; 5.
DR   InterPro; IPR002713; FF_domain.
DR   InterPro; IPR036517; FF_domain_sf.
DR   InterPro; IPR039726; Prp40-like.
DR   InterPro; IPR001202; WW_dom.
DR   InterPro; IPR036020; WW_dom_sf.
DR   PANTHER; PTHR11864; PTHR11864; 1.
DR   Pfam; PF01846; FF; 4.
DR   Pfam; PF00397; WW; 2.
DR   SMART; SM00441; FF; 5.
DR   SMART; SM00456; WW; 2.
DR   SUPFAM; SSF51045; SSF51045; 2.
DR   SUPFAM; SSF81698; SSF81698; 5.
DR   PROSITE; PS51676; FF; 6.
DR   PROSITE; PS50020; WW_DOMAIN_2; 2.
PE   1: Evidence at protein level;
KW   mRNA processing; mRNA splicing; Nucleus; Phosphoprotein;
KW   Reference proteome; Repeat; Ribonucleoprotein.
FT   CHAIN           1..695
FT                   /note="Pre-mRNA-processing protein prp40"
FT                   /id="PRO_0000234563"
FT   DOMAIN          30..63
FT                   /note="WW 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00224"
FT   DOMAIN          71..104
FT                   /note="WW 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00224"
FT   DOMAIN          191..245
FT                   /note="FF 1"
FT   DOMAIN          257..313
FT                   /note="FF 2"
FT   DOMAIN          326..385
FT                   /note="FF 3"
FT   DOMAIN          403..465
FT                   /note="FF 4"
FT   DOMAIN          470..521
FT                   /note="FF 5"
FT   DOMAIN          544..601
FT                   /note="FF 6"
FT   REGION          1..35
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          120..165
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..28
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        148..165
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         688
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18257517"
FT   MOD_RES         689
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18257517"
SQ   SEQUENCE   695 AA;  81931 MW;  CB5D333C4DB486B5 CRC64;
     MSAPPWQTSE YDETEGFTSN QEGPSAAPSK TVASDWHEVK TEDSRVYYYN SVTRKSVWEK
     PEELMNDFEK KLSKLAWKEY ATADGKKYWY NVNTRESVWD IPDEYKAALV DEPEQQKKAL
     SSKIKSNDNK PAVQSIQRHG PDVAAPSSQP AKDQSQQISQ GSHKRTINFV QQKDKRQKRS
     NDYQHENYDT YEAAERAFFK FLDSHNVNPS WTWEQTVREL CDAKGYYVMK DPWHRKCAFD
     AYILNYLTDQ SDAEKNRVTK IRKEFIEMLK SSDKIHSYTL WRTVKNEFSS HPAFNATSSE
     TEQQQLFFEY KQKLLEDEKQ LEKDRRKEAL DDFCSLLRNM NFEPYTRWSV AQAKFDQDPR
     YTRNSNMKYL SKLDALVAFE DHVKHLEREY ILDKQKQKKE KHRIERKNRD AFRALLQDLR
     VQKKITLRTK WKELYPIIKD DPRYLNLLGQ SGSTPLDLFW DTIVDLENMY REKRNLVLDC
     LEVLQISVDD TSNIPEIIAR LSEKLKDREE SEAVTEDLIE EVVNRLRDKA IHKKAEEKRA
     DERRIRRKID NLRSAIKYLK PPISADASYD EIRPLISILP EFAALHSEEH RMAAFDKYIR
     RLREKRELEK QYQNRRGYYD VGKDESYLAN SARPHSGYED GRLEYSADLA SKSNRNEINT
     MQDVQENSIS HVTATQPAVK NIVDDAESSE EGEIR