ID   PRP46_ASHGO             Reviewed;         425 AA.
AC   Q75BY3;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 2.
DT   25-MAY-2022, entry version 113.
DE   RecName: Full=Pre-mRNA-splicing factor PRP46;
DE   AltName: Full=Pre-mRNA-processing protein 46;
GN   Name=PRP46; OrderedLocusNames=ACR137W;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION, AND SEQUENCE REVISION TO 64 AND 79.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Involved in pre-mRNA splicing and required for cell cycle
CC       progression at G2/M. {ECO:0000250}.
CC   -!- SUBUNIT: Associated with the spliceosome. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the WD repeat PRL1/PRL2 family. {ECO:0000305}.
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DR   EMBL; AE016816; AAS51363.2; -; Genomic_DNA.
DR   RefSeq; NP_983539.2; NM_208892.2.
DR   AlphaFoldDB; Q75BY3; -.
DR   SMR; Q75BY3; -.
DR   STRING; 33169.AAS51363; -.
DR   EnsemblFungi; AAS51363; AAS51363; AGOS_ACR137W.
DR   GeneID; 4619671; -.
DR   KEGG; ago:AGOS_ACR137W; -.
DR   eggNOG; KOG0285; Eukaryota.
DR   HOGENOM; CLU_000288_72_0_1; -.
DR   InParanoid; Q75BY3; -.
DR   OMA; HPLEWKP; -.
DR   Proteomes; UP000000591; Chromosome III.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0071014; C:post-mRNA release spliceosomal complex; IEA:EnsemblFungi.
DR   GO; GO:0000974; C:Prp19 complex; IBA:GO_Central.
DR   GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IEA:EnsemblFungi.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR045241; Prp46/PLRG1-like.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR19923; PTHR19923; 1.
DR   Pfam; PF00400; WD40; 4.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00320; WD40; 7.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 2.
DR   PROSITE; PS50082; WD_REPEATS_2; 4.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW   Repeat; Spliceosome; WD repeat.
FT   CHAIN           1..425
FT                   /note="Pre-mRNA-splicing factor PRP46"
FT                   /id="PRO_0000051159"
FT   REPEAT          111..151
FT                   /note="WD 1"
FT   REPEAT          154..193
FT                   /note="WD 2"
FT   REPEAT          196..235
FT                   /note="WD 3"
FT   REPEAT          238..279
FT                   /note="WD 4"
FT   REPEAT          281..320
FT                   /note="WD 5"
FT   REPEAT          322..360
FT                   /note="WD 6"
FT   REPEAT          371..410
FT                   /note="WD 7"
SQ   SEQUENCE   425 AA;  48021 MW;  DE24F7D912F00485 CRC64;
     MNEHSDDVYV RARLRNQFGY MTWVPEYVED RISSKKGILQ RYEDYQQKQA KAQEVKTDSL
     VKYEGAKDVP RNLLRIYRGE ADTSALARYE EVVSQKPQWH APWKLTRVIN GHTGWVRCVC
     VDPVDNAWFA TGSNDSTIRV WDLATGKLKV TLQGHIMTVR DICISARHPY MFSASQDKLV
     KCWDLERNTV VRDFHGTLSG VHSVDLHPSL DLIVSAGRDS VVRVWDIRSR SCVLTLAGHR
     GPINKVRCLP VDPQIVSCST DATVKLWDLV AGKPMKTLTH HKRNVRDLAF NPTEFSFASA
     CTDDIRSWKL VDGQLLTNFN SEALGIVNTL ACNQDGVLFA GGDTGELSFF DYKTGHKFQK
     LETTAMPGSL ESEKGVLAST FDRTGLRLLT CERDKSIKIW KHIDGATQDS HPGLPWNPSL
     VRQRF