ID   PRP46_CRYNJ             Reviewed;         473 AA.
AC   P0CS48; Q55PP3; Q5KDR4;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 51.
DE   RecName: Full=Pre-mRNA-splicing factor PRP46;
DE   AltName: Full=Pre-mRNA-processing protein 46;
GN   Name=PRP46; OrderedLocusNames=CNG03060;
OS   Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS   MYA-565) (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=214684;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JEC21 / ATCC MYA-565;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: Involved in pre-mRNA splicing and required for cell cycle
CC       progression at G2/M. {ECO:0000250}.
CC   -!- SUBUNIT: Associated with the spliceosome. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the WD repeat PRL1/PRL2 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE017347; AAW44697.1; -; Genomic_DNA.
DR   RefSeq; XP_572004.1; XM_572004.1.
DR   AlphaFoldDB; P0CS48; -.
DR   SMR; P0CS48; -.
DR   STRING; 5207.AAW44697; -.
DR   PaxDb; P0CS48; -.
DR   EnsemblFungi; AAW44697; AAW44697; CNG03060.
DR   GeneID; 3258829; -.
DR   KEGG; cne:CNG03060; -.
DR   VEuPathDB; FungiDB:CNG03060; -.
DR   eggNOG; KOG0285; Eukaryota.
DR   HOGENOM; CLU_000288_72_2_1; -.
DR   InParanoid; P0CS48; -.
DR   OMA; HPLEWKP; -.
DR   OrthoDB; 1157847at2759; -.
DR   Proteomes; UP000002149; Chromosome 7.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0000974; C:Prp19 complex; IBA:GO_Central.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR045241; Prp46/PLRG1-like.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR19923; PTHR19923; 1.
DR   Pfam; PF00400; WD40; 7.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00320; WD40; 7.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 2.
DR   PROSITE; PS50082; WD_REPEATS_2; 5.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW   Repeat; Spliceosome; WD repeat.
FT   CHAIN           1..473
FT                   /note="Pre-mRNA-splicing factor PRP46"
FT                   /id="PRO_0000051163"
FT   REPEAT          180..219
FT                   /note="WD 1"
FT   REPEAT          222..261
FT                   /note="WD 2"
FT   REPEAT          264..303
FT                   /note="WD 3"
FT   REPEAT          306..347
FT                   /note="WD 4"
FT   REPEAT          349..388
FT                   /note="WD 5"
FT   REPEAT          391..429
FT                   /note="WD 6"
FT   REPEAT          440..473
FT                   /note="WD 7"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          103..126
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   473 AA;  50896 MW;  1FFA49D2D30D6FD4 CRC64;
     MSTSLETPSG ASAGPSIVAS GLPPLADLVR AGSKRTRVVY GAETSAVEDD GLARANKIKL
     ATKLAIEYKD VQTLPPILQA QQTGPAGPKR PTQPSIAASA TAGPNVKLIG GPEAEKASSS
     TPQAVAEPRS LVKFRHQEGF AAEGGQATSR LSQALMRKKE AREVKPEYHP EWKLTRVISG
     HMGWVRAVAM DPGNQWFATG AGDRVIKIWD LASGELKLSL TGHISTIRGL AVSDRHPYLF
     SCAEDKMVKC WDLETNKVIR HYHGHFSGVY SLSVHPTLDV LVTGGRDASV RVWDMRTRAN
     IFTLTGHTST VGDVKTQDSD PQIISGSMDS TVRLWDLAAG KCMNTLTHHK KSVRALAIHP
     TEYSFASASS GGNNIKKWKC PEGIFVNNFV GHEAIINTLS INSENVLFSG ADNGTLTLWD
     YKTGLPFQHL KDIPQPGSLD AEAGVFCSTF DKTGTRLITG GADKTIKVYS EQA