ID   PRP46_SCHPO             Reviewed;         473 AA.
AC   O13615;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 165.
DE   RecName: Full=Pre-mRNA-splicing factor prp5;
DE   AltName: Full=Complexed with cdc5 protein 1;
DE   AltName: Full=Pre-mRNA-processing protein 5;
GN   Name=prp5; Synonyms=cwf1; ORFNames=pi024, SPBP22H7.07;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 26-47, AND FUNCTION.
RX   PubMed=10409726; DOI=10.1128/mcb.19.8.5352;
RA   McDonald W.H., Ohi R., Smelkova N., Frendewey D., Gould K.L.;
RT   "Myb-related fission yeast cdc5p is a component of a 40S snRNP-containing
RT   complex and is essential for pre-mRNA splicing.";
RL   Mol. Cell. Biol. 19:5352-5362(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=10620777;
RX   DOI=10.1002/(sici)1097-0061(20000115)16:1<71::aid-yea505>3.0.co;2-5;
RA   Machida M., Yamazaki S., Kunihiro S., Tanaka T., Kushida N., Jinno K.,
RA   Haikawa Y., Yamazaki J., Yamamoto S., Sekine M., Oguchi A., Nagai Y.,
RA   Sakai M., Aoki K., Ogura K., Kudoh Y., Kikuchi H., Zhang M.Q., Yanagida M.;
RT   "A 38 kb segment containing the cdc2 gene from the left arm of fission
RT   yeast chromosome II: sequence analysis and characterization of the genomic
RT   DNA and cDNAs encoded on the segment.";
RL   Yeast 16:71-80(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [4]
RP   FUNCTION.
RX   PubMed=9745017; DOI=10.1007/s002940050381;
RA   Potashkin J., Kim D., Fons M., Humphrey T., Frendewey D.;
RT   "Cell-division-cycle defects associated with fission yeast pre-mRNA
RT   splicing mutants.";
RL   Curr. Genet. 34:153-163(1998).
RN   [5]
RP   GENETIC INTERACTION WITH PRP4.
RX   PubMed=11252721; DOI=10.1093/embo-reports/kve009;
RA   Schwelnus W., Richert K., Opitz F., Gross T., Habara Y., Tani T.,
RA   Kaeufer N.F.;
RT   "Fission yeast Prp4p kinase regulates pre-mRNA splicing by phosphorylating
RT   a non-SR-splicing factor.";
RL   EMBO Rep. 2:35-41(2001).
RN   [6]
RP   IDENTIFICATION IN THE CWF COMPLEX, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=11884590; DOI=10.1128/mcb.22.7.2011-2024.2002;
RA   Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.;
RT   "Proteomics analysis reveals stable multiprotein complexes in both fission
RT   and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA
RT   splicing factors, and snRNAs.";
RL   Mol. Cell. Biol. 22:2011-2024(2002).
CC   -!- FUNCTION: Required for both cell cycle progression at G2/M and pre-mRNA
CC       splicing. Interacts genetically with the PRP4 kinase.
CC       {ECO:0000269|PubMed:10409726, ECO:0000269|PubMed:9745017}.
CC   -!- SUBUNIT: Belongs to the 40S cdc5-associated complex (or cwf complex), a
CC       spliceosome sub-complex reminiscent of a late-stage spliceosome
CC       composed of the U2, U5 and U6 snRNAs and at least brr2, cdc5,
CC       cwf2/prp3, cwf3/syf1, cwf4/syf3, cwf5/ecm2, spp42/cwf6, cwf7/spf27,
CC       cwf8, cwf9, cwf10, cwf11, cwf12, prp45/cwf13, cwf14, cwf15, cwf16,
CC       cwf17, cwf18, cwf19, cwf20, cwf21, cwf22, cwf23, cwf24, cwf25, cwf26,
CC       cyp7/cwf27, cwf28, cwf29/ist3, lea1, msl1, prp5/cwf1, prp10,
CC       prp12/sap130, prp17, prp22, sap61, sap62, sap114, sap145, slu7, smb1,
CC       smd1, smd3, smf1, smg1 and syf2. {ECO:0000269|PubMed:11884590}.
CC   -!- INTERACTION:
CC       O13615; P39964: cdc5; NbExp=7; IntAct=EBI-538787, EBI-538771;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the WD repeat PRL1/PRL2 family. {ECO:0000305}.
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DR   EMBL; AF251148; AAG01399.1; -; Genomic_DNA.
DR   EMBL; AB004535; BAA21403.1; -; Genomic_DNA.
DR   EMBL; CU329671; CAC37375.1; -; Genomic_DNA.
DR   RefSeq; NP_595604.1; NM_001021499.2.
DR   PDB; 3JB9; EM; 3.60 A; K=149-473.
DR   PDBsum; 3JB9; -.
DR   AlphaFoldDB; O13615; -.
DR   SMR; O13615; -.
DR   BioGRID; 280336; 25.
DR   IntAct; O13615; 8.
DR   STRING; 4896.SPBP22H7.07.1; -.
DR   iPTMnet; O13615; -.
DR   MaxQB; O13615; -.
DR   PaxDb; O13615; -.
DR   PRIDE; O13615; -.
DR   EnsemblFungi; SPBP22H7.07.1; SPBP22H7.07.1:pep; SPBP22H7.07.
DR   GeneID; 3361260; -.
DR   KEGG; spo:SPBP22H7.07; -.
DR   PomBase; SPBP22H7.07; prp5.
DR   VEuPathDB; FungiDB:SPBP22H7.07; -.
DR   eggNOG; KOG0285; Eukaryota.
DR   HOGENOM; CLU_000288_72_2_1; -.
DR   InParanoid; O13615; -.
DR   OMA; HPLEWKP; -.
DR   PhylomeDB; O13615; -.
DR   PRO; PR:O13615; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0071014; C:post-mRNA release spliceosomal complex; IDA:PomBase.
DR   GO; GO:0000974; C:Prp19 complex; IDA:PomBase.
DR   GO; GO:0005681; C:spliceosomal complex; IDA:PomBase.
DR   GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IMP:PomBase.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR045241; Prp46/PLRG1-like.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR19923; PTHR19923; 1.
DR   Pfam; PF00400; WD40; 6.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00320; WD40; 7.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 2.
DR   PROSITE; PS50082; WD_REPEATS_2; 5.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; mRNA processing; mRNA splicing;
KW   Nucleus; Reference proteome; Repeat; Spliceosome; WD repeat.
FT   CHAIN           1..473
FT                   /note="Pre-mRNA-splicing factor prp5"
FT                   /id="PRO_0000051167"
FT   REPEAT          161..191
FT                   /note="WD 1"
FT   REPEAT          203..233
FT                   /note="WD 2"
FT   REPEAT          245..275
FT                   /note="WD 3"
FT   REPEAT          287..317
FT                   /note="WD 4"
FT   REPEAT          329..358
FT                   /note="WD 5"
FT   REPEAT          370..399
FT                   /note="WD 6"
FT   REPEAT          419..449
FT                   /note="WD 7"
SQ   SEQUENCE   473 AA;  52418 MW;  941F3B31A8C19799 CRC64;
     MTEAKNISDD TDVLSLTTNS LRTSKTLFGA EFGSVTSFDD TVAQNLKRTY KEHLEYGSVL
     GGTVGKRKNR HYEEDTIGSN ALTVRADSEN PSSQVITKFS DPNKKIAGQV SMQSLEKIKG
     VPEAAHRIAG ESQASLVKRT LAEQIRPEWH APWTLMRVIS GHLGWVRCVD VEPGNQWFCT
     GAGDRTIKIW DLASGVLKLT LTGHIATVRG LAVSPRHPYL FSCGEDKMVK CWDLETNKVI
     RHYHGHLSGV YALKLHPTLD VLVTAGRDAV ARVWDMRTRQ NVHVLSGHKS TVASLAVQEF
     DPQVVTGSMD STIRLWDLAA GKTLTTLTHH KKTVRALSLH PDEFTFASGS SDNIKHWKFP
     EGAFMGNFEG HNAIVNTLSI NSDNVMFSGA DNGSMCFWDW KSGHKYQELQ SVVQPGSLDS
     EAGIFASSFD KTGLRLITCE ADKSVKIYKQ VDNATPETHP NLPWTPSNLR RRY