ID   PRP4_BOVIN              Reviewed;         521 AA.
AC   Q3MHE2;
DT   23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2005, sequence version 1.
DT   03-AUG-2022, entry version 120.
DE   RecName: Full=U4/U6 small nuclear ribonucleoprotein Prp4;
DE   AltName: Full=U4/U6 snRNP 60 kDa protein;
DE   AltName: Full=WD splicing factor Prp4;
GN   Name=PRPF4;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Ascending colon;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays role in pre-mRNA splicing as component of the U4/U6-U5
CC       tri-snRNP complex that is involved in spliceosome assembly, and as
CC       component of the precatalytic spliceosome (spliceosome B complex).
CC       {ECO:0000250|UniProtKB:O43172}.
CC   -!- SUBUNIT: Component of the precatalytic spliceosome (spliceosome B
CC       complex) (By similarity). Component of the U4/U6-U5 tri-snRNP complex,
CC       a building block of the precatalytic spliceosome (spliceosome B
CC       complex) (By similarity). The U4/U6-U5 tri-snRNP complex is composed of
CC       the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8,
CC       PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3,
CC       SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and
CC       USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8 (By
CC       similarity). Interacts directly with PRPF18, PPIH and PRPF3 (By
CC       similarity). Part of a heteromeric complex containing PPIH, PRPF3 and
CC       PRPF4 that is stable in the absence of RNA (By similarity). Interacts
CC       with ERCC6 (By similarity). {ECO:0000250|UniProtKB:O43172}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O43172}. Nucleus
CC       speckle {ECO:0000250|UniProtKB:O43172}.
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DR   EMBL; BC105270; AAI05271.1; -; mRNA.
DR   RefSeq; NP_001029502.1; NM_001034330.1.
DR   AlphaFoldDB; Q3MHE2; -.
DR   SMR; Q3MHE2; -.
DR   STRING; 9913.ENSBTAP00000006004; -.
DR   PaxDb; Q3MHE2; -.
DR   PRIDE; Q3MHE2; -.
DR   Ensembl; ENSBTAT00000006004; ENSBTAP00000006004; ENSBTAG00000004571.
DR   GeneID; 508735; -.
DR   KEGG; bta:508735; -.
DR   CTD; 9128; -.
DR   VEuPathDB; HostDB:ENSBTAG00000004571; -.
DR   VGNC; VGNC:33378; PRPF4.
DR   eggNOG; KOG0272; Eukaryota.
DR   GeneTree; ENSGT00940000156006; -.
DR   HOGENOM; CLU_000288_57_20_1; -.
DR   InParanoid; Q3MHE2; -.
DR   OMA; LNEPICY; -.
DR   OrthoDB; 1076587at2759; -.
DR   TreeFam; TF314922; -.
DR   Proteomes; UP000009136; Chromosome 8.
DR   Bgee; ENSBTAG00000004571; Expressed in oviduct epithelium and 108 other tissues.
DR   GO; GO:0015030; C:Cajal body; IEA:Ensembl.
DR   GO; GO:0016607; C:nuclear speck; ISS:UniProtKB.
DR   GO; GO:0097525; C:spliceosomal snRNP complex; ISS:UniProtKB.
DR   GO; GO:0071005; C:U2-type precatalytic spliceosome; IEA:Ensembl.
DR   GO; GO:0071001; C:U4/U6 snRNP; ISS:UniProtKB.
DR   GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; IBA:GO_Central.
DR   GO; GO:0030621; F:U4 snRNA binding; IBA:GO_Central.
DR   GO; GO:0017070; F:U6 snRNA binding; IBA:GO_Central.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR   Gene3D; 2.130.10.10; -; 3.
DR   Gene3D; 4.10.280.110; -; 1.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR014906; PRP4-like.
DR   InterPro; IPR036285; PRP4-like_sf.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   Pfam; PF08799; PRP4; 1.
DR   Pfam; PF00400; WD40; 6.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00500; SFM; 1.
DR   SMART; SM00320; WD40; 7.
DR   SUPFAM; SSF158230; SSF158230; 1.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 2.
DR   PROSITE; PS50082; WD_REPEATS_2; 6.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW   Repeat; Spliceosome; WD repeat.
FT   CHAIN           1..521
FT                   /note="U4/U6 small nuclear ribonucleoprotein Prp4"
FT                   /id="PRO_0000273725"
FT   REPEAT          229..268
FT                   /note="WD 1"
FT   REPEAT          271..318
FT                   /note="WD 2"
FT   REPEAT          321..360
FT                   /note="WD 3"
FT   REPEAT          363..402
FT                   /note="WD 4"
FT   REPEAT          405..444
FT                   /note="WD 5"
FT   REPEAT          447..487
FT                   /note="WD 6"
FT   REPEAT          490..521
FT                   /note="WD 7"
FT   MOD_RES         26
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:O43172"
SQ   SEQUENCE   521 AA;  58399 MW;  326D366F9F1CBF59 CRC64;
     MASTRPSSTA TKTKAPDDLV APVVKKPHIY YGSLEEKERE RLAKGESGLL GKEGLKAGIE
     AGNINITSGE VFEIEEHISE RQAEVLAEFE RRKRARQINV STDDSEVKAC LRALGEPITL
     FGEGPAERRE RLRNILSVVG TDALKKTKKD DEKSKKSKEE YQQTWYHEGP HSLKVARLWI
     ANYSLPRAMK RLEEARLHKE IPETTRTSQM QELHKSLRSL NNFCSQIGDD RPISYCHFSP
     NSKMLATACW SGLCKLWSVP DCNLLHTLRG HNTNVGAIVF HPKSTVSLDQ KDVNLASCAA
     DGSVKLWSLD SDEPVADIEG HTVRVARVTW HPSGRFLGTT CYDRSWRLWD LEAQEEILHQ
     EGHSMGVYDI AFHQDGSLAG TGGLDAFGRV WDLRTGRCIM FLEGHLKEIY GINFSPNGYH
     IATGSGDNTC KVWDLRQRRC VYTIPAHQNL VTGVKFEPIH GNFLLTGAYD NTAKIWTHPG
     WSPLKTLAGH EGKVMGLDIS SDGQLIATCS YDRTFKLWMA E