PRP4_MOUSE
ID PRP4_MOUSE Reviewed; 521 AA.
AC Q9DAW6;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=U4/U6 small nuclear ribonucleoprotein Prp4;
DE AltName: Full=U4/U6 snRNP 60 kDa protein;
DE AltName: Full=WD splicing factor Prp4;
GN Name=Prpf4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Placenta, Spleen, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-26, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic fibroblast;
RX PubMed=23806337; DOI=10.1016/j.molcel.2013.06.001;
RA Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y.,
RA Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.;
RT "SIRT5-mediated lysine desuccinylation impacts diverse metabolic
RT pathways.";
RL Mol. Cell 50:919-930(2013).
CC -!- FUNCTION: Plays role in pre-mRNA splicing as component of the U4/U6-U5
CC tri-snRNP complex that is involved in spliceosome assembly, and as
CC component of the precatalytic spliceosome (spliceosome B complex).
CC {ECO:0000250|UniProtKB:O43172}.
CC -!- SUBUNIT: Component of the precatalytic spliceosome (spliceosome B
CC complex) (By similarity). Component of the U4/U6-U5 tri-snRNP complex,
CC a building block of the precatalytic spliceosome (spliceosome B
CC complex) (By similarity). The U4/U6-U5 tri-snRNP complex is composed of
CC the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8,
CC PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3,
CC SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and
CC USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8 (By
CC similarity). Interacts directly with PRPF18, PPIH and PRPF3 (By
CC similarity). Part of a heteromeric complex containing PPIH, PRPF3 and
CC PRPF4 that is stable in the absence of RNA (By similarity). Interacts
CC with ERCC6 (By similarity). {ECO:0000250|UniProtKB:O43172}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O43172}. Nucleus
CC speckle {ECO:0000250|UniProtKB:O43172}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK005464; BAB24055.1; -; mRNA.
DR EMBL; AK089838; BAC40976.1; -; mRNA.
DR EMBL; AK166912; BAE39112.1; -; mRNA.
DR EMBL; AK167216; BAE39342.1; -; mRNA.
DR EMBL; AK169532; BAE41214.1; -; mRNA.
DR EMBL; BC106089; AAI06090.1; -; mRNA.
DR CCDS; CCDS18239.1; -.
DR RefSeq; NP_081573.1; NM_027297.3.
DR AlphaFoldDB; Q9DAW6; -.
DR SMR; Q9DAW6; -.
DR BioGRID; 213838; 13.
DR IntAct; Q9DAW6; 1.
DR MINT; Q9DAW6; -.
DR STRING; 10090.ENSMUSP00000081572; -.
DR iPTMnet; Q9DAW6; -.
DR PhosphoSitePlus; Q9DAW6; -.
DR EPD; Q9DAW6; -.
DR jPOST; Q9DAW6; -.
DR MaxQB; Q9DAW6; -.
DR PaxDb; Q9DAW6; -.
DR PRIDE; Q9DAW6; -.
DR ProteomicsDB; 291792; -.
DR Antibodypedia; 15276; 138 antibodies from 23 providers.
DR DNASU; 70052; -.
DR Ensembl; ENSMUST00000084524; ENSMUSP00000081572; ENSMUSG00000066148.
DR GeneID; 70052; -.
DR KEGG; mmu:70052; -.
DR UCSC; uc008ter.1; mouse.
DR CTD; 9128; -.
DR MGI; MGI:1917302; Prpf4.
DR VEuPathDB; HostDB:ENSMUSG00000066148; -.
DR eggNOG; KOG0272; Eukaryota.
DR GeneTree; ENSGT00940000156006; -.
DR HOGENOM; CLU_000288_57_20_1; -.
DR InParanoid; Q9DAW6; -.
DR OMA; LNEPICY; -.
DR OrthoDB; 1076587at2759; -.
DR PhylomeDB; Q9DAW6; -.
DR TreeFam; TF314922; -.
DR Reactome; R-MMU-72163; mRNA Splicing - Major Pathway.
DR BioGRID-ORCS; 70052; 28 hits in 75 CRISPR screens.
DR ChiTaRS; Prpf4; mouse.
DR PRO; PR:Q9DAW6; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q9DAW6; protein.
DR Bgee; ENSMUSG00000066148; Expressed in manus and 213 other tissues.
DR ExpressionAtlas; Q9DAW6; baseline and differential.
DR Genevisible; Q9DAW6; MM.
DR GO; GO:0015030; C:Cajal body; ISO:MGI.
DR GO; GO:0016607; C:nuclear speck; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0097525; C:spliceosomal snRNP complex; ISS:UniProtKB.
DR GO; GO:0071005; C:U2-type precatalytic spliceosome; ISO:MGI.
DR GO; GO:0071001; C:U4/U6 snRNP; ISS:UniProtKB.
DR GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; ISO:MGI.
DR GO; GO:0030621; F:U4 snRNA binding; IBA:GO_Central.
DR GO; GO:0017070; F:U6 snRNA binding; IBA:GO_Central.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISO:MGI.
DR Gene3D; 2.130.10.10; -; 3.
DR Gene3D; 4.10.280.110; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR014906; PRP4-like.
DR InterPro; IPR036285; PRP4-like_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF08799; PRP4; 1.
DR Pfam; PF00400; WD40; 6.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00500; SFM; 1.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF158230; SSF158230; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 6.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Acetylation; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW Repeat; Spliceosome; WD repeat.
FT CHAIN 1..521
FT /note="U4/U6 small nuclear ribonucleoprotein Prp4"
FT /id="PRO_0000051150"
FT REPEAT 228..267
FT /note="WD 1"
FT REPEAT 270..317
FT /note="WD 2"
FT REPEAT 320..359
FT /note="WD 3"
FT REPEAT 362..401
FT /note="WD 4"
FT REPEAT 404..443
FT /note="WD 5"
FT REPEAT 446..486
FT /note="WD 6"
FT REPEAT 489..521
FT /note="WD 7"
FT MOD_RES 26
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:23806337"
SQ SEQUENCE 521 AA; 58370 MW; CDE80E76D591CC89 CRC64;
MASSRASSTT TKTKAPDDLV APVVKKPHIY YGSLEEKERE RLAKGESGIL GKEGLKAGIE
AGNINITSGE VFEIEEHISE RQAEVLAEFE RRKRARQINV STDDSEVKAC LRALGEPITL
FGEGPAERRE RLRNILSVVG TDALKKTKKD DEKSKKSKEE YQQTWYHEGP NSLKVARLWI
ANYSLPRAMK RLEEARLHKE IPETTRTSQM QELHKSLRSL NNFCSQIGDD RPISYCHFSP
NSKMLATACW SGLCKLWSVP DCSLLHTLRG HNTNVGAIVF HPKSTVSLDQ KDVNLASCAA
DGSVKLWSLD SDEPVADIEG HTVRVARVMW HPSGRFLGTT CYDRSWRLWD LEAQEEILHQ
EGHSMGVYDI AFHQDGSLAG TGGLDAFGRV WDLRTGRCIM FLEGHLKEIY GINFSPNGYH
IATGSGDNTC KVWDLRQRRC VYTIPAHQNL VTGVKFEPIH GDFLLTGAYD NTAKIWTHPG
WSPLKTLAGH EGKVMGLDIS SDGQLIATCS YDRTFKLWMA E
