ID   PRP4_PONAB              Reviewed;         521 AA.
AC   Q5NVD0;
DT   10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   04-JAN-2005, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=U4/U6 small nuclear ribonucleoprotein Prp4;
DE   AltName: Full=U4/U6 snRNP 60 kDa protein;
DE   AltName: Full=WD splicing factor Prp4;
GN   Name=PRPF4;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain cortex;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays role in pre-mRNA splicing as component of the U4/U6-U5
CC       tri-snRNP complex that is involved in spliceosome assembly, and as
CC       component of the precatalytic spliceosome (spliceosome B complex).
CC       {ECO:0000250|UniProtKB:O43172}.
CC   -!- SUBUNIT: Component of the precatalytic spliceosome (spliceosome B
CC       complex) (By similarity). Component of the U4/U6-U5 tri-snRNP complex,
CC       a building block of the precatalytic spliceosome (spliceosome B
CC       complex) (By similarity). The U4/U6-U5 tri-snRNP complex is composed of
CC       the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8,
CC       PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3,
CC       SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and
CC       USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8 (By
CC       similarity). Interacts directly with PRPF18, PPIH and PRPF3 (By
CC       similarity). Part of a heteromeric complex containing PPIH, PRPF3 and
CC       PRPF4 that is stable in the absence of RNA (By similarity). Interacts
CC       with ERCC6 (By similarity). {ECO:0000250|UniProtKB:O43172}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O43172}. Nucleus
CC       speckle {ECO:0000250|UniProtKB:O43172}.
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DR   EMBL; CR926107; CAI29733.1; -; mRNA.
DR   RefSeq; NP_001127122.1; NM_001133650.1.
DR   AlphaFoldDB; Q5NVD0; -.
DR   SMR; Q5NVD0; -.
DR   GeneID; 100174168; -.
DR   KEGG; pon:100174168; -.
DR   CTD; 9128; -.
DR   InParanoid; Q5NVD0; -.
DR   Proteomes; UP000001595; Unplaced.
DR   GO; GO:0016607; C:nuclear speck; ISS:UniProtKB.
DR   GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
DR   GO; GO:0097525; C:spliceosomal snRNP complex; ISS:UniProtKB.
DR   GO; GO:0071001; C:U4/U6 snRNP; ISS:UniProtKB.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   Gene3D; 2.130.10.10; -; 3.
DR   Gene3D; 4.10.280.110; -; 1.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR014906; PRP4-like.
DR   InterPro; IPR036285; PRP4-like_sf.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   Pfam; PF08799; PRP4; 1.
DR   Pfam; PF00400; WD40; 6.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00500; SFM; 1.
DR   SMART; SM00320; WD40; 7.
DR   SUPFAM; SSF158230; SSF158230; 1.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 2.
DR   PROSITE; PS50082; WD_REPEATS_2; 6.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW   Repeat; Spliceosome; WD repeat.
FT   CHAIN           1..521
FT                   /note="U4/U6 small nuclear ribonucleoprotein Prp4"
FT                   /id="PRO_0000051151"
FT   REPEAT          228..267
FT                   /note="WD 1"
FT   REPEAT          270..317
FT                   /note="WD 2"
FT   REPEAT          320..359
FT                   /note="WD 3"
FT   REPEAT          362..401
FT                   /note="WD 4"
FT   REPEAT          404..443
FT                   /note="WD 5"
FT   REPEAT          446..486
FT                   /note="WD 6"
FT   REPEAT          489..521
FT                   /note="WD 7"
FT   MOD_RES         26
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:O43172"
SQ   SEQUENCE   521 AA;  58336 MW;  BA7A0223EE94C024 CRC64;
     MASSRASSTA TKTKAPDDLV APVVKKPHIY YGSLEEKERE RLAKGESGIL GKEGLKAGIE
     AGNINITSGE VFEIEEHISE RQAEVLAEFE RRKRARQINV STDDSEVKAC LRALGEPITL
     FGEGPAERRE RLRNILSVVG TDALKKTKKD DEKSKKSKEE YQQTWYHEGP NSLKVARLWI
     ANYSLPRAMK RLEEARLHKE IPETTRASQM QELHKSLRSL NNFCSQIGDD RPISYCHFSP
     NSKMLATACW SGLCKLWSVP DCNLLHTLRG HNTNVGAIVF HPKSTVSLDQ KDVNLASCAA
     DGSVKLWSLD SDEPVADIEG HTVRVARVMW HPSGRFLGTT CYDRSWRLWD LEAQEEILHQ
     EGHSMGVYDI AFHQDGSLAG TGGLDAFGRV WDLRTGRCIM FLEGHLKEIY GINFSPNGYH
     IATGSGDNTC KVWDLRQRRC VYTIPAHQNL VTGVKFEPIH GNFLLTGAYD NTAKIWTHPG
     WSPLKTLAGH EGKVMGLDIS SDGQLIATCS YDRTFKLWMA E