PRP4_YEAST
ID PRP4_YEAST Reviewed; 465 AA.
AC P20053; D6W4H8;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 03-AUG-2022, entry version 188.
DE RecName: Full=U4/U6 small nuclear ribonucleoprotein PRP4;
DE AltName: Full=Pre-mRNA-processing protein 4;
GN Name=PRP4; Synonyms=RNA4; OrderedLocusNames=YPR178W; ORFNames=P9705.6;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2528686; DOI=10.1128/mcb.9.9.3698-3709.1989;
RA Petersen-Bjoern S., Soltyk A., Beggs J.D., Friesen J.D.;
RT "PRP4 (RNA4) from Saccharomyces cerevisiae: its gene product is associated
RT with the U4/U6 small nuclear ribonucleoprotein particle.";
RL Mol. Cell. Biol. 9:3698-3709(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2528687; DOI=10.1128/mcb.9.9.3710-3719.1989;
RA Banroques J., Abelson J.N.;
RT "PRP4: a protein of the yeast U4/U6 small nuclear ribonucleoprotein
RT particle.";
RL Mol. Cell. Biol. 9:3710-3719(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=10449419; DOI=10.1093/emboj/18.16.4535;
RA Gottschalk A., Neubauer G., Banroques J., Mann M., Luehrmann R.,
RA Fabrizio P.;
RT "Identification by mass spectrometry and functional analysis of novel
RT proteins of the yeast [U4/U6.U5] tri-snRNP.";
RL EMBO J. 18:4535-4548(1999).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
CC -!- FUNCTION: Involved in RNA splicing. Is required for the association of
CC U4/U6 snRNP with U5 snRNP in an early step of spliceosome assembly.
CC -!- SUBUNIT: Component of the U4/U6-U5 tri-snRNP complex composed of the
CC U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31,
CC PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3,
CC SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1.
CC {ECO:0000269|PubMed:10449419}.
CC -!- INTERACTION:
CC P20053; P47093: LSM8; NbExp=3; IntAct=EBI-219, EBI-313;
CC P20053; Q03338: PRP3; NbExp=4; IntAct=EBI-219, EBI-421;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 1770 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; M26597; AAA79332.1; -; Genomic_DNA.
DR EMBL; M28518; AAA79011.1; -; Genomic_DNA.
DR EMBL; U25842; AAB68111.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11594.1; -; Genomic_DNA.
DR PIR; A32569; A32569.
DR RefSeq; NP_015504.1; NM_001184275.1.
DR PDB; 3JCM; EM; 3.80 A; B=1-465.
DR PDB; 5GAN; EM; 3.60 A; H=1-465.
DR PDB; 5GAP; EM; 3.60 A; H=1-465.
DR PDB; 5NRL; EM; 7.20 A; H=1-465.
DR PDB; 5ZWM; EM; 3.40 A; K=1-465.
DR PDB; 5ZWO; EM; 3.90 A; K=1-465.
DR PDBsum; 3JCM; -.
DR PDBsum; 5GAN; -.
DR PDBsum; 5GAP; -.
DR PDBsum; 5NRL; -.
DR PDBsum; 5ZWM; -.
DR PDBsum; 5ZWO; -.
DR AlphaFoldDB; P20053; -.
DR SMR; P20053; -.
DR BioGRID; 36350; 566.
DR ComplexPortal; CPX-25; U4/U6.U5 tri-small nuclear ribonucleoprotein complex.
DR ComplexPortal; CPX-31; U4 small nuclear ribonucleoprotein complex.
DR ComplexPortal; CPX-32; U4/U6 small nuclear ribonucleoprotein complex.
DR DIP; DIP-2607N; -.
DR IntAct; P20053; 46.
DR MINT; P20053; -.
DR STRING; 4932.YPR178W; -.
DR iPTMnet; P20053; -.
DR MaxQB; P20053; -.
DR PaxDb; P20053; -.
DR PRIDE; P20053; -.
DR EnsemblFungi; YPR178W_mRNA; YPR178W; YPR178W.
DR GeneID; 856307; -.
DR KEGG; sce:YPR178W; -.
DR SGD; S000006382; PRP4.
DR VEuPathDB; FungiDB:YPR178W; -.
DR eggNOG; KOG0272; Eukaryota.
DR GeneTree; ENSGT00940000156006; -.
DR HOGENOM; CLU_000288_57_20_1; -.
DR InParanoid; P20053; -.
DR OMA; LNEPICY; -.
DR BioCyc; YEAST:G3O-34303-MON; -.
DR PRO; PR:P20053; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; P20053; protein.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0005681; C:spliceosomal complex; IC:ComplexPortal.
DR GO; GO:0005687; C:U4 snRNP; IC:ComplexPortal.
DR GO; GO:0071001; C:U4/U6 snRNP; IC:ComplexPortal.
DR GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; IDA:SGD.
DR GO; GO:0030621; F:U4 snRNA binding; IBA:GO_Central.
DR GO; GO:0017070; F:U6 snRNA binding; IBA:GO_Central.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0034247; P:snoRNA splicing; IMP:SGD.
DR GO; GO:0000245; P:spliceosomal complex assembly; IC:ComplexPortal.
DR GO; GO:0000393; P:spliceosomal conformational changes to generate catalytic conformation; IMP:SGD.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR014906; PRP4-like.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 4.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00500; SFM; 1.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 3.
DR PROSITE; PS50082; WD_REPEATS_2; 4.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW 3D-structure; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW Repeat; Spliceosome; WD repeat.
FT CHAIN 1..465
FT /note="U4/U6 small nuclear ribonucleoprotein PRP4"
FT /id="PRO_0000051152"
FT REPEAT 173..212
FT /note="WD 1"
FT REPEAT 216..256
FT /note="WD 2"
FT REPEAT 263..302
FT /note="WD 3"
FT REPEAT 305..344
FT /note="WD 4"
FT REPEAT 347..386
FT /note="WD 5"
FT REPEAT 391..432
FT /note="WD 6"
FT REPEAT 435..464
FT /note="WD 7"
SQ SEQUENCE 465 AA; 52442 MW; 7CCCA3A92AC0CE78 CRC64;
MSKYIALENL PVDLQHKGAT QNESTADILK QLPHERLQAV LEKIPEEDLE VRRLLSILKK
PEVVENEDVQ QRRIRLAEIL MVDEIDLENI NNMENINGEE VDEEDDEDFF TPATSELIFA
RRFLINYSLE RSRKRLQKEM ERHQKFNTRQ ELLSRRTELQ RMANLELAGS QLVSTKPISA
VSLSTDDMVV ATGSWAGDLQ VLNSQTLQPL TQKLDSHVGK IGAIDWHPDS NNQMISCAED
GLIKNFQYSN EEGGLRLLGD LVGHERRISD VKYHPSGKFI GSASHDMTWR LWDASTHQEL
LLQEGHDKGV FSLSFQCDGS LVCSGGMDSL SMLWDIRSGS KVMTLAGHSK PIYTVAWSPN
GYQVATGGGD GIINVWDIRK RDEGQLNQIL AHRNIVTQVR FSKEDGGKKL VSCGYDNLIN
VYSSDTWLKM GSLAGHTDKI ISLDISNNSH FLVSGGWDRS IKLWN
