PRP6_BOVIN
ID PRP6_BOVIN Reviewed; 941 AA.
AC Q2KJJ0;
DT 19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT 07-MAR-2006, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Pre-mRNA-processing factor 6;
DE AltName: Full=PRP6 homolog;
DE AltName: Full=U5 snRNP-associated 102 kDa protein;
DE Short=U5-102 kDa protein;
GN Name=PRPF6;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hereford;
RX PubMed=19390049; DOI=10.1126/science.1169588;
RG The bovine genome sequencing and analysis consortium;
RT "The genome sequence of taurine cattle: a window to ruminant biology and
RT evolution.";
RL Science 324:522-528(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Ileum;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in pre-mRNA splicing as component of the U4/U6-U5
CC tri-snRNP complex, one of the building blocks of the spliceosome.
CC Enhances dihydrotestosterone-induced transactivation activity of AR, as
CC well as dexamethasone-induced transactivation activity of NR3C1, but
CC does not affect estrogen-induced transactivation.
CC {ECO:0000250|UniProtKB:O94906}.
CC -!- SUBUNIT: Identified in the spliceosome B complex. Identified in the
CC spliceosome C complex. Associates with the U5 snRNP particle. Component
CC of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs
CC and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A,
CC SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, LSm
CC proteins LSm2-8 and Sm proteins. Interacts with ARAF1. Interacts with
CC AR and NR3C1, but not ESR1, independently of the presence of hormones.
CC Interacts with USH1G. {ECO:0000250|UniProtKB:O94906}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm
CC {ECO:0000250|UniProtKB:O94906}. Nucleus speckle
CC {ECO:0000250|UniProtKB:O94906}. Note=Localized in splicing speckles.
CC {ECO:0000250|UniProtKB:O94906}.
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DR EMBL; AAFC03042419; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC105318; AAI05319.1; -; mRNA.
DR RefSeq; NP_001039840.1; NM_001046375.2.
DR AlphaFoldDB; Q2KJJ0; -.
DR SMR; Q2KJJ0; -.
DR STRING; 9913.ENSBTAP00000015480; -.
DR PaxDb; Q2KJJ0; -.
DR PRIDE; Q2KJJ0; -.
DR Ensembl; ENSBTAT00000081159; ENSBTAP00000074220; ENSBTAG00000011656.
DR GeneID; 534228; -.
DR KEGG; bta:534228; -.
DR CTD; 24148; -.
DR VEuPathDB; HostDB:ENSBTAG00000011656; -.
DR VGNC; VGNC:33382; PRPF6.
DR eggNOG; KOG0495; Eukaryota.
DR GeneTree; ENSGT00550000075016; -.
DR HOGENOM; CLU_007010_0_0_1; -.
DR InParanoid; Q2KJJ0; -.
DR OMA; DGWAWYY; -.
DR OrthoDB; 335779at2759; -.
DR TreeFam; TF105743; -.
DR Proteomes; UP000009136; Chromosome 13.
DR Bgee; ENSBTAG00000011656; Expressed in laryngeal cartilage and 107 other tissues.
DR ExpressionAtlas; Q2KJJ0; baseline.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR GO; GO:0016607; C:nuclear speck; ISS:UniProtKB.
DR GO; GO:0071005; C:U2-type precatalytic spliceosome; ISS:UniProtKB.
DR GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; IBA:GO_Central.
DR GO; GO:0005682; C:U5 snRNP; IEA:Ensembl.
DR GO; GO:0042802; F:identical protein binding; IEA:Ensembl.
DR GO; GO:0050681; F:nuclear androgen receptor binding; IEA:Ensembl.
DR GO; GO:0043021; F:ribonucleoprotein complex binding; IEA:Ensembl.
DR GO; GO:0003723; F:RNA binding; IEA:Ensembl.
DR GO; GO:0003713; F:transcription coactivator activity; IEA:Ensembl.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:Ensembl.
DR GO; GO:0006403; P:RNA localization; IEA:Ensembl.
DR GO; GO:0000244; P:spliceosomal tri-snRNP complex assembly; IBA:GO_Central.
DR Gene3D; 1.25.40.10; -; 4.
DR InterPro; IPR003107; HAT.
DR InterPro; IPR010491; PRP1_N.
DR InterPro; IPR027108; Prp6/Prp1/STA1.
DR InterPro; IPR045075; Syf1-like.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR PANTHER; PTHR11246; PTHR11246; 1.
DR PANTHER; PTHR11246:SF1; PTHR11246:SF1; 1.
DR Pfam; PF06424; PRP1_N; 1.
DR SMART; SM00386; HAT; 13.
DR SMART; SM00028; TPR; 3.
DR SUPFAM; SSF48452; SSF48452; 4.
DR PROSITE; PS50293; TPR_REGION; 2.
PE 2: Evidence at transcript level;
KW mRNA processing; mRNA splicing; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; Spliceosome.
FT CHAIN 1..941
FT /note="Pre-mRNA-processing factor 6"
FT /id="PRO_0000413094"
FT REPEAT 384..416
FT /note="HAT 1"
FT REPEAT 418..444
FT /note="HAT 2"
FT REPEAT 445..476
FT /note="HAT 3"
FT REPEAT 554..586
FT /note="HAT 4"
FT REPEAT 588..620
FT /note="HAT 5"
FT REPEAT 622..654
FT /note="HAT 6"
FT REPEAT 689..721
FT /note="HAT 7"
FT REPEAT 723..755
FT /note="HAT 8"
FT REPEAT 855..887
FT /note="HAT 9"
FT REGION 1..79
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 38..68
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 143
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O94906"
FT MOD_RES 180
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O94906"
FT MOD_RES 266
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O94906"
FT MOD_RES 275
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O94906"
FT MOD_RES 279
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O94906"
SQ SEQUENCE 941 AA; 106678 MW; 09461950CA494F23 CRC64;
MNKKKKPFLG MPAPLGYVPG LGRGATGFTT RSDIGPARDA NDPVDDRHAP PGKRTVGDQM
KKSQAADDDD EDLNDTNYDE FNGYAGSLFS SGPYEKDDEE ADAIYAALDK RMDERRKERR
EQREKEEIEK YRMERPKIQQ QFSDLKRKLA EVTEEEWLSI PEVGDARNKR QRNPRYEKLT
PVPDSFFAKH LQTGENHTSV DPRQTQFGGL NTPYPGGLNT PYPGGMTPGL MTPGTGELDM
RKIGQARNTL MDMRLSQVSD SVSGQTVVDP KGYLTDLNSM IPTHGGDIND IKKARLLLKS
VRETNPHHPP AWIASARLEE VTGKLQVARN LIMKGTEMCP KSEDVWLEAA RLQPGDTAKA
VVAQAVRHLP QSVRIYIRAA ELETDIRAKK RVLRKALEHV PNSVRLWKAA VELEEPEDAR
IMLSRAVECC PTSVELWLAL ARLETYENAR KVLNKARENI PTDRHIWITA AKLEEANGNT
QMVEKIIDRA ITSLRANGVE INREQWIQDA EECDKAGSVA TCQAVMRAVI GIGIEEEDRK
HTWMEDADSC VAHNALECAR AIYAYALQVF PSKKSVWLRA AYFEKNHGTR ESLEALLQRA
VAHCPKAEVL WLMGAKSKWL AGDVPAARSI LALAFQANPN SEEIWLAAVK LESENNEYER
ARRLLAKARS SAPTARVFMK SVKLEWVLGN LVAAQELCEE ALKHYEDFPK LWMMKGQIEE
QEELVEKARE AYNQGLKKCP HSTPLWLLLS RLEEKVGQLT RARAILEKSR LKNPKNPGLW
LESVRLEYRA GLKNIASTLM AKALQECPNS GVLWSEAIFL EARPQRKTKS VDALKKCEHD
PHVLLAVAKL FWSERKITKA REWFHRTVKI DSDLGDAWAF FYKFELQHGT EEQREEVRRR
CENAEPRHGE LWCATSKDIA NWQRKIGEIL VLVAARIKNT F
