PRP6_YEAST
ID PRP6_YEAST Reviewed; 899 AA.
AC P19735; D6VQ54;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 03-AUG-2022, entry version 186.
DE RecName: Full=Pre-mRNA-splicing factor 6;
GN Name=PRP6; Synonyms=RNA6; OrderedLocusNames=YBR055C; ORFNames=YBR0508;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=S288c / GRF88;
RX PubMed=2118103; DOI=10.1002/j.1460-2075.1990.tb07465.x;
RA Legrain P., Choulika A.;
RT "The molecular characterization of PRP6 and PRP9 yeast genes reveals a new
RT cysteine/histidine motif common to several splicing factors.";
RL EMBO J. 9:2775-2781(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7597852; DOI=10.1002/yea.320110511;
RA Aljinovic G., Pohl T.M.;
RT "Sequence and analysis of 24 kb on chromosome II of Saccharomyces
RT cerevisiae.";
RL Yeast 11:475-479(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=10449419; DOI=10.1093/emboj/18.16.4535;
RA Gottschalk A., Neubauer G., Banroques J., Mann M., Luehrmann R.,
RA Fabrizio P.;
RT "Identification by mass spectrometry and functional analysis of novel
RT proteins of the yeast [U4/U6.U5] tri-snRNP.";
RL EMBO J. 18:4535-4548(1999).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, AND ELECTRON
RP MICROSCOPY.
RX PubMed=18953335; DOI=10.1038/nsmb.1506;
RA Hacker I., Sander B., Golas M.M., Wolf E., Karagoz E., Kastner B.,
RA Stark H., Fabrizio P., Luhrmann R.;
RT "Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-
RT snRNP by electron microscopy.";
RL Nat. Struct. Mol. Biol. 15:1206-1212(2008).
CC -!- FUNCTION: Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-
CC snRNP complex, one of the building blocks of the spliceosome.
CC -!- SUBUNIT: Component of the U4/U6-U5 tri-snRNP complex composed of the
CC U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31,
CC PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3,
CC SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1.
CC {ECO:0000269|PubMed:10449419, ECO:0000269|PubMed:18953335}.
CC -!- INTERACTION:
CC P19735; Q06819: DIB1; NbExp=3; IntAct=EBI-227, EBI-166;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- MISCELLANEOUS: Present with 2250 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; X53465; CAA37559.1; -; Genomic_DNA.
DR EMBL; Z35924; CAA84998.1; -; Genomic_DNA.
DR EMBL; Z46260; CAA86398.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07174.1; -; Genomic_DNA.
DR PIR; S12319; S12319.
DR RefSeq; NP_009611.1; NM_001178403.1.
DR PDB; 3JCM; EM; 3.80 A; G=1-899.
DR PDB; 5GAN; EM; 3.60 A; J=1-899.
DR PDB; 5GAP; EM; 3.60 A; J=1-899.
DR PDB; 5NRL; EM; 7.20 A; J=1-899.
DR PDB; 5ZWM; EM; 3.40 A; N=1-899.
DR PDB; 5ZWO; EM; 3.90 A; N=1-899.
DR PDBsum; 3JCM; -.
DR PDBsum; 5GAN; -.
DR PDBsum; 5GAP; -.
DR PDBsum; 5NRL; -.
DR PDBsum; 5ZWM; -.
DR PDBsum; 5ZWO; -.
DR AlphaFoldDB; P19735; -.
DR SMR; P19735; -.
DR BioGRID; 32756; 381.
DR ComplexPortal; CPX-25; U4/U6.U5 tri-small nuclear ribonucleoprotein complex.
DR ComplexPortal; CPX-29; U5 small nuclear ribonucleoprotein complex.
DR DIP; DIP-1290N; -.
DR IntAct; P19735; 111.
DR MINT; P19735; -.
DR STRING; 4932.YBR055C; -.
DR MaxQB; P19735; -.
DR PaxDb; P19735; -.
DR PRIDE; P19735; -.
DR EnsemblFungi; YBR055C_mRNA; YBR055C; YBR055C.
DR GeneID; 852344; -.
DR KEGG; sce:YBR055C; -.
DR SGD; S000000259; PRP6.
DR VEuPathDB; FungiDB:YBR055C; -.
DR eggNOG; KOG0495; Eukaryota.
DR GeneTree; ENSGT00940000172817; -.
DR HOGENOM; CLU_007010_0_0_1; -.
DR InParanoid; P19735; -.
DR OMA; DGWAWYY; -.
DR BioCyc; YEAST:G3O-29026-MON; -.
DR PRO; PR:P19735; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P19735; protein.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0005681; C:spliceosomal complex; IC:ComplexPortal.
DR GO; GO:0071001; C:U4/U6 snRNP; IMP:GO_Central.
DR GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; IDA:SGD.
DR GO; GO:0005682; C:U5 snRNP; IC:ComplexPortal.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IMP:GO_Central.
DR GO; GO:0000244; P:spliceosomal tri-snRNP complex assembly; IBA:GO_Central.
DR Gene3D; 1.25.40.10; -; 3.
DR InterPro; IPR003107; HAT.
DR InterPro; IPR010491; PRP1_N.
DR InterPro; IPR027108; Prp6/Prp1/STA1.
DR InterPro; IPR045075; Syf1-like.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR PANTHER; PTHR11246; PTHR11246; 1.
DR PANTHER; PTHR11246:SF1; PTHR11246:SF1; 1.
DR Pfam; PF06424; PRP1_N; 1.
DR SMART; SM00386; HAT; 7.
DR SMART; SM00028; TPR; 3.
DR SUPFAM; SSF48452; SSF48452; 3.
PE 1: Evidence at protein level;
KW 3D-structure; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW Repeat; Spliceosome.
FT CHAIN 1..899
FT /note="Pre-mRNA-splicing factor 6"
FT /id="PRO_0000205756"
FT REPEAT 221..253
FT /note="HAT 1"
FT REPEAT 255..287
FT /note="HAT 2"
FT REPEAT 289..318
FT /note="HAT 3"
FT REPEAT 319..350
FT /note="HAT 4"
FT REPEAT 352..381
FT /note="HAT 5"
FT REPEAT 383..414
FT /note="HAT 6"
FT REPEAT 493..525
FT /note="HAT 7"
FT REPEAT 545..578
FT /note="HAT 8"
FT REPEAT 608..645
FT /note="HAT 9"
FT REPEAT 648..680
FT /note="HAT 10"
FT REPEAT 682..714
FT /note="HAT 11"
FT REPEAT 751..783
FT /note="HAT 12"
FT REPEAT 819..851
FT /note="HAT 13"
FT REGION 1..65
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 32..65
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 899 AA; 104229 MW; 3E87F8AF63EAAC41 CRC64;
MERPSFLDQE PPAGYVPGIG RGATGFSTKE KQVVSNDDKG RRIPKRYREN LNNHLQSQPK
DDEDDEAANV FKTLELKLAQ KKKKRANEKD DDNSVDSSNV KRQFADLKES LAAVTESEWM
DIPDATDFTR RNKRNRIQEQ LNRKTYAAPD SLIPGNVDLN KLTEEREKLL QSQIDENLAQ
LTKNASNPIQ VNKPNAATDA LSYLKDLEND RVNSLSDATL EDLQKMRTIL KSYRKADPTN
PQGWIASARL EEKARKFSVA KKIIENGCQE CPRSSDIWLE NIRLHESDVH YCKTLVATAI
NFNPTSPLLW FKAIDLESTT VNKYRVVRKA LQEIPRDEGL WKLAVSFEAD KAQVIKMLEK
ATQFIPQSMD LLTAYTNLQS YHNAKMTLNS FRKILPQEPE IWIISTLLEE RNNPDIPVDK
LVSLLKEGLL ELSKNGYKAT LSAWLKRAEA LNDAPNSNLT CQAIVYAILE WLRESGEYES
ELNNVDQILE KMPHSKVQIA VLKKLIQWDP CDTVLWSRLK MATESYHKIE ELLAFFQELL
FQTKNSDDIR ANMREKSPGL LMMYVSEYWK AQKGDTRQTL VLIDQIIDFA PHNLDLRFFK
IKLLGRSLQL DELRDFFQQT FSSLEDFKIS GTERLYYKYV NFLRYQDLNE EAIKFLNERC
LKSFPICHKF FLQLGQIYHS MGNIEMSRET YLSGTRLVPN CPLLWVSLSK IDEIDLKNPV
RARSILDRGL LKNPDDVLFY IAKIQMEIRL GNLDQAELLV TQALQKFPSN ALLWVEQIKL
FKHGNKSSLK KTIFQDALRR TQNDHRVLLE IGVSFYAEAQ YETSLKWLER ALKKCSRYGD
TWVWLFRTYA RLGKDTVDLY NMFDQCEPTY GPEWIAASKN VKMQYCTPRE ILLRLMNDK
