PRP8A_ARATH
ID PRP8A_ARATH Reviewed; 2359 AA.
AC Q9SSD2;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 137.
DE RecName: Full=Pre-mRNA-processing-splicing factor 8A {ECO:0000305};
DE AltName: Full=PRP8 homolog A {ECO:0000305};
DE Short=AtPRP8A {ECO:0000303|PubMed:19261069};
DE AltName: Full=Protein ABNORMAL SUSPENSOR 2 {ECO:0000303|Ref.3};
DE AltName: Full=Protein EMBRYO DEFECTIVE 14 {ECO:0000303|Ref.3};
DE AltName: Full=Protein EMBRYO DEFECTIVE 177 {ECO:0000303|Ref.3};
DE AltName: Full=Protein EMBRYO DEFECTIVE 33 {ECO:0000303|Ref.3};
DE AltName: Full=Protein SUPPRESSOR OF OVEREXPRESSED FCA 81 {ECO:0000303|PubMed:24725596};
GN Name=PRP8A {ECO:0000303|PubMed:19261069};
GN Synonyms=EMB14 {ECO:0000303|Ref.3}, EMB177 {ECO:0000303|Ref.3},
GN EMB33 {ECO:0000303|Ref.3}, SOF81 {ECO:0000303|PubMed:24725596},
GN SUS2 {ECO:0000303|Ref.3};
GN OrderedLocusNames=At1g80070 {ECO:0000312|Araport:AT1G80070};
GN ORFNames=F18B13.15 {ECO:0000312|EMBL:AAD55467.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP DISRUPTION PHENOTYPE.
RA Schwartz B.W., Yeung E.C., Meinke D.W.;
RT "Disruption of morphogenesis and transformation of the suspensor in
RT abnormal suspensor mutants of Arabidopsis.";
RL Development 120:3235-3245(1994).
RN [4]
RP INTERACTION WITH CLO.
RX PubMed=19261069; DOI=10.1111/j.1744-7909.2008.00783.x;
RA Liu M., Yuan L., Liu N.Y., Shi D.Q., Liu J., Yang W.C.;
RT "GAMETOPHYTIC FACTOR 1, involved in pre-mRNA splicing, is essential for
RT megagametogenesis and embryogenesis in Arabidopsis.";
RL J. Integr. Plant Biol. 51:261-271(2009).
RN [5]
RP FUNCTION.
RX PubMed=24725596; DOI=10.1016/j.molcel.2014.03.026;
RA Marquardt S., Raitskin O., Wu Z., Liu F., Sun Q., Dean C.;
RT "Functional consequences of splicing of the antisense transcript COOLAIR on
RT FLC transcription.";
RL Mol. Cell 54:156-165(2014).
CC -!- FUNCTION: Functions as a scaffold that mediates the ordered assembly of
CC spliceosomal proteins and snRNAs. Required for the assembly of the
CC U4/U6-U5 tri-snRNP complex (By similarity). Required for embryo
CC development (Ref.3). Required for splicing efficiency of COOLAIR
CC introns and usage of the proximal poly(A) site. COOLAIR is a set of
CC long non-coding antisense transcripts produced at the FLOWERING LOCUS C
CC (FLC). COOLAIR initiates just downstream of the major sense transcript
CC poly(A) site and terminates either early or extends into the FLC
CC promoter region. Splicing of COOLAIR by PRP8A is functionally important
CC for FLC regulation (PubMed:24725596). {ECO:0000250|UniProtKB:Q6P2Q9,
CC ECO:0000269|PubMed:24725596, ECO:0000269|Ref.3}.
CC -!- SUBUNIT: Interacts with CLO. {ECO:0000269|PubMed:19261069}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Embryonic lethality due to abnormal suspensor
CC development. {ECO:0000269|Ref.3}.
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DR EMBL; AC009322; AAD55467.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE36353.1; -; Genomic_DNA.
DR PIR; B96832; B96832.
DR RefSeq; NP_178124.2; NM_106656.4.
DR AlphaFoldDB; Q9SSD2; -.
DR SMR; Q9SSD2; -.
DR IntAct; Q9SSD2; 4.
DR STRING; 3702.AT1G80070.1; -.
DR iPTMnet; Q9SSD2; -.
DR PaxDb; Q9SSD2; -.
DR PRIDE; Q9SSD2; -.
DR ProteomicsDB; 226294; -.
DR EnsemblPlants; AT1G80070.1; AT1G80070.1; AT1G80070.
DR GeneID; 844347; -.
DR Gramene; AT1G80070.1; AT1G80070.1; AT1G80070.
DR KEGG; ath:AT1G80070; -.
DR Araport; AT1G80070; -.
DR TAIR; locus:2016289; AT1G80070.
DR eggNOG; KOG1795; Eukaryota.
DR HOGENOM; CLU_000380_3_0_1; -.
DR InParanoid; Q9SSD2; -.
DR OMA; VCMRREK; -.
DR OrthoDB; 156083at2759; -.
DR PhylomeDB; Q9SSD2; -.
DR PRO; PR:Q9SSD2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SSD2; baseline and differential.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0005682; C:U5 snRNP; IBA:GO_Central.
DR GO; GO:0070122; F:isopeptidase activity; IEA:InterPro.
DR GO; GO:0140492; F:metal-dependent deubiquitinase activity; IEA:InterPro.
DR GO; GO:0097157; F:pre-mRNA intronic binding; IBA:GO_Central.
DR GO; GO:0030619; F:U1 snRNA binding; IBA:GO_Central.
DR GO; GO:0030620; F:U2 snRNA binding; IBA:GO_Central.
DR GO; GO:0030623; F:U5 snRNA binding; IBA:GO_Central.
DR GO; GO:0017070; F:U6 snRNA binding; IBA:GO_Central.
DR GO; GO:0000380; P:alternative mRNA splicing, via spliceosome; IMP:TAIR.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IMP:UniProtKB.
DR GO; GO:0000244; P:spliceosomal tri-snRNP complex assembly; IBA:GO_Central.
DR CDD; cd13838; RNase_H_like_Prp8_IV; 1.
DR Gene3D; 1.20.80.40; -; 1.
DR Gene3D; 3.30.420.230; -; 1.
DR Gene3D; 3.30.43.40; -; 1.
DR InterPro; IPR000555; JAMM/MPN+_dom.
DR InterPro; IPR037518; MPN.
DR InterPro; IPR012591; PRO8NT.
DR InterPro; IPR012592; PROCN.
DR InterPro; IPR012984; PROCT.
DR InterPro; IPR027652; PRP8.
DR InterPro; IPR021983; PRP8_domainIV.
DR InterPro; IPR043173; Prp8_domainIV_fingers.
DR InterPro; IPR043172; Prp8_domainIV_palm.
DR InterPro; IPR019581; Prp8_U5-snRNA-bd.
DR InterPro; IPR042516; Prp8_U5-snRNA-bd_sf.
DR InterPro; IPR019580; Prp8_U6-snRNA-bd.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR019582; RRM_spliceosomal_PrP8.
DR PANTHER; PTHR11140; PTHR11140; 1.
DR Pfam; PF01398; JAB; 1.
DR Pfam; PF08082; PRO8NT; 1.
DR Pfam; PF08083; PROCN; 1.
DR Pfam; PF08084; PROCT; 1.
DR Pfam; PF12134; PRP8_domainIV; 1.
DR Pfam; PF10598; RRM_4; 1.
DR Pfam; PF10597; U5_2-snRNA_bdg; 1.
DR Pfam; PF10596; U6-snRNA_bdg; 1.
DR SMART; SM00232; JAB_MPN; 1.
DR SUPFAM; SSF53098; SSF53098; 2.
DR PROSITE; PS50249; MPN; 1.
PE 1: Evidence at protein level;
KW mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW Ribonucleoprotein; RNA-binding; Spliceosome.
FT CHAIN 1..2359
FT /note="Pre-mRNA-processing-splicing factor 8A"
FT /id="PRO_0000436563"
FT DOMAIN 2127..2258
FT /note="MPN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01182"
FT REGION 1..54
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 15..46
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2359 AA; 275427 MW; A2E2A74BCB024DE3 CRC64;
MWNNNDGMPL APPGTGGSMM PPPPAAHPSY TALPPPSNPT PPVEPTPEEA EAKLEEKARK
WMQLNSKRYG DKRKFGFVET QKEDMPPEHV RKIIRDHGDM SSKKFRHDKR VYLGALKFVP
HAVFKLLENM PMPWEQVRDV KVLYHITGAI TFVNEIPWVV EPIYMAQWGT MWIMMRREKR
DRRHFKRMRF PPFDDEEPPL DYADNLLDVD PLEPIQLELD EEEDSAVHTW FYDHKPLVKT
KLINGPSYRR WNLSLPIMAT LHRLAGQLLS DLIDRNYFYL FDMPSFFTAK ALNMCIPGGP
KFEPLYRDME KGDEDWNEFN DINKLIIRSP LRTEYRIAFP HLYNNRPRKV KLCVYHSPMI
MYIKTEDPDL PAFYYDPLIH PISNTNKEKR ERKVYDDEDD FALPEGVEPL LRDTQLYTDT
TAAGISLLFA PRPFNMRSGR TRRAEDIPLV SEWFKEHCPP AYPVKVRVSY QKLLKCYVLN
ELHHRPPKAQ KKKHLFRSLA ATKFFQSTEL DWVEVGLQVC RQGYNMLNLL IHRKNLNYLH
LDYNFNLKPV KTLTTKERKK SRFGNAFHLC REILRLTKLV VDANVQFRLG NVDAFQLADG
LQYIFSHVGQ LTGMYRYKYR LMRQIRMCKD LKHLIYYRFN TGPVGKGPGC GFWAPMWRVW
LFFLRGIVPL LERWLGNLLA RQFEGRHSKG VAKTVTKQRV ESHFDLELRA AVMHDVLDAM
PEGIKQNKAR TILQHLSEAW RCWKANIPWK VPGLPVPIEN MILRYVKSKA DWWTNVAHYN
RERIRRGATV DKTVCRKNLG RLTRLWLKAE QERQHNYLKD GPYVTPEEAL AIYTTTVHWL
ESRKFSPIPF PPLSYKHDTK LLILALERLK ESYSVAVRLN QQQREELGLI EQAYDNPHEA
LSRIKRHLLT QRGFKEVGIE FMDLYSYLIP VYEIEPLEKI TDAYLDQYLW YEGDKRHLFP
NWIKPADSEP PPLLVYKWCQ GINNLQGIWD TGDGQCVVML QTKFEKFFEK IDLTMLNRLL
RLVLDHNIAD YVSAKNNVVL SYKDMSHTNS YGLIRGLQFA SFVVQFYGLL LDLLLLGLTR
ASEIAGPPQM PNEFMTFWDT KVETRHPIRL YSRYIDKVHI MFKFTHEEAR DLIQRYLTEH
PDPNNENMVG YNNKKCWPRD ARMRLMKHDV NLGRSVFWDM KNRLPRSITT LEWENGFVSV
YSKDNPNLLF SMCGFEVRIL PKIRMTQEAF SNTKDGVWNL QNEQTKERTA VAFLRVDDEH
MKVFENRVRQ ILMSSGSTTF TKIVNKWNTA LIGLMTYFRE ATVHTQELLD LLVKCENKIQ
TRIKIGLNSK MPSRFPPVIF YTPKEIGGLG MLSMGHILIP QSDLRYSKQT DVGVTHFRSG
MSHEEDQLIP NLYRYIQPWE SEFIDSQRVW AEYALKRQEA QAQNRRLTLE DLEDSWDRGI
PRINTLFQKD RHTLAYDKGW RVRTDFKQYQ VLKQNPFWWT HQRHDGKLWN LNNYRTDVIQ
ALGGVEGILE HTLFKGTYFP TWEGLFWEKA SGFEESMKYK KLTNAQRSGL NQIPNRRFTL
WWSPTINRAN VYVGFQVQLD LTGIFMHGKI PTLKISLIQI FRAHLWQKIH ESVVMDLCQV
LDQELDALEI ETVQKETIHP RKSYKMNSSC ADVLLFAAHK WPMSKPSLVA ESKDMFDQKA
SNKYWIDVQL RWGDYDSHDI ERYTRAKFMD YTTDNMSIYP SPTGVMIGLD LAYNLHSAFG
NWFPGSKPLL AQAMNKIMKS NPALYVLRER IRKGLQLYSS EPTEPYLSSQ NYGEIFSNQI
IWFVDDTNVY RVTIHKTFEG NLTTKPINGA IFIFNPRTGQ LFLKVIHTSV WAGQKRLGQL
AKWKTAEEVA ALVRSLPVEE QPKQIIVTRK GMLDPLEVHL LDFPNIVIKG SELQLPFQAC
LKIEKFGDLI LKATEPQMVL FNIYDDWLKS ISSYTAFSRL ILILRALHVN NEKAKMLLKP
DKSVVTEPHH IWPSLTDDQW MKVEVALRDL ILSDYAKKNN VNTSALTQSE IRDIILGAEI
TPPSQQRQQI AEIEKQAKEA SQLTAVTTRT TNVHGDELIV TTTSPYEQSA FGSKTDWRVR
AISATNLYLR VNHIYVNSDD IKETGYTYIM PKNILKKFIC VADLRTQIAG YLYGISPPDN
PQVKEIRCVV MVPQWGNHQL VHLPSSLPEH DFLNDLEPLG WLHTQPNELP QLSPQDVTSH
SRILENNKQW DGEKCIILTC SFTPGSCSLT SYKLTQTGYE WGRLNKDNGS NPHGYLPTHY
EKVQMLLSDR FLGFYMVPES GPWNYSFTGV KHTLSMKYSV KLGSPKEFYH EEHRPTHFLE
FSNMEEADIT EGDREDTFT
