PRP8B_ARATH
ID PRP8B_ARATH Reviewed; 2359 AA.
AC Q9T0I6; F4JUG5;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 06-JUL-2016, sequence version 2.
DT 25-MAY-2022, entry version 132.
DE RecName: Full=Pre-mRNA-processing-splicing factor 8B {ECO:0000305};
DE AltName: Full=PRP8 homolog B {ECO:0000305};
DE Short=AtPRP8B {ECO:0000305};
GN Name=PRP8B {ECO:0000305};
GN OrderedLocusNames=At4g38780 {ECO:0000312|Araport:AT4G38780};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: Functions as a scaffold that mediates the ordered assembly of
CC spliceosomal proteins and snRNAs. Required for the assembly of the
CC U4/U6-U5 tri-snRNP complex. {ECO:0000250|UniProtKB:Q6P2Q9}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AEE86973.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB38612.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB80541.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL035656; CAB38612.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161594; CAB80541.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE86973.1; ALT_SEQ; Genomic_DNA.
DR PIR; T06077; T06077.
DR RefSeq; NP_195589.2; NM_120038.5.
DR AlphaFoldDB; Q9T0I6; -.
DR SMR; Q9T0I6; -.
DR IntAct; Q9T0I6; 2.
DR STRING; 3702.AT4G38780.1; -.
DR PeptideAtlas; Q9T0I6; -.
DR PRIDE; Q9T0I6; -.
DR ProteomicsDB; 226380; -.
DR GeneID; 830033; -.
DR KEGG; ath:AT4G38780; -.
DR Araport; AT4G38780; -.
DR TAIR; locus:2141791; AT4G38780.
DR eggNOG; KOG1795; Eukaryota.
DR OrthoDB; 156083at2759; -.
DR PRO; PR:Q9T0I6; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9T0I6; baseline and differential.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0005682; C:U5 snRNP; IBA:GO_Central.
DR GO; GO:0070122; F:isopeptidase activity; IEA:InterPro.
DR GO; GO:0140492; F:metal-dependent deubiquitinase activity; IEA:InterPro.
DR GO; GO:0003729; F:mRNA binding; HDA:TAIR.
DR GO; GO:0097157; F:pre-mRNA intronic binding; IBA:GO_Central.
DR GO; GO:0030619; F:U1 snRNA binding; IBA:GO_Central.
DR GO; GO:0030620; F:U2 snRNA binding; IBA:GO_Central.
DR GO; GO:0030623; F:U5 snRNA binding; IBA:GO_Central.
DR GO; GO:0017070; F:U6 snRNA binding; IBA:GO_Central.
DR GO; GO:0000244; P:spliceosomal tri-snRNP complex assembly; IBA:GO_Central.
DR CDD; cd13838; RNase_H_like_Prp8_IV; 1.
DR Gene3D; 1.20.80.40; -; 1.
DR Gene3D; 3.30.420.230; -; 1.
DR Gene3D; 3.30.43.40; -; 1.
DR InterPro; IPR000555; JAMM/MPN+_dom.
DR InterPro; IPR037518; MPN.
DR InterPro; IPR012591; PRO8NT.
DR InterPro; IPR012592; PROCN.
DR InterPro; IPR012984; PROCT.
DR InterPro; IPR027652; PRP8.
DR InterPro; IPR021983; PRP8_domainIV.
DR InterPro; IPR043173; Prp8_domainIV_fingers.
DR InterPro; IPR043172; Prp8_domainIV_palm.
DR InterPro; IPR019581; Prp8_U5-snRNA-bd.
DR InterPro; IPR042516; Prp8_U5-snRNA-bd_sf.
DR InterPro; IPR019580; Prp8_U6-snRNA-bd.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR019582; RRM_spliceosomal_PrP8.
DR PANTHER; PTHR11140; PTHR11140; 1.
DR Pfam; PF01398; JAB; 1.
DR Pfam; PF08082; PRO8NT; 1.
DR Pfam; PF08083; PROCN; 1.
DR Pfam; PF08084; PROCT; 1.
DR Pfam; PF12134; PRP8_domainIV; 1.
DR Pfam; PF10598; RRM_4; 1.
DR Pfam; PF10597; U5_2-snRNA_bdg; 1.
DR Pfam; PF10596; U6-snRNA_bdg; 1.
DR SMART; SM00232; JAB_MPN; 1.
DR SUPFAM; SSF53098; SSF53098; 2.
DR PROSITE; PS50249; MPN; 1.
PE 3: Inferred from homology;
KW mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW Ribonucleoprotein; RNA-binding; Spliceosome.
FT CHAIN 1..2359
FT /note="Pre-mRNA-processing-splicing factor 8B"
FT /id="PRO_0000436564"
FT DOMAIN 2129..2260
FT /note="MPN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01182"
FT REGION 1..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 13..43
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2359 AA; 274802 MW; 0ED001DE6C36ABAD CRC64;
MWNIDGTSLA PPGTDGSRMQ TPSHPADHPS YTAPSNRNTP TVPTPEDAEA KLEKKARTWM
QLNSKRYGDK RKFGFVETQK EDMPPEHVRK IIRDHGDMSS KKHRLDKRVY LGALKFVPHA
VFKLLENMPM PWEQVRDVKV LYHITGAITF VNEVRWVVEP IYMAQWGSMW IMMRREKRDR
RHFKRMRFPP FDDEEPPLDY ADNLLDVDPL EAIQLELDEE EDSAVYSWFY DHKPLVKTKM
INGPSYQTWN LSLPIMSTLH RLAAQLLSDL VDRNYFYLFD MPSFFTAKAL NMCIPGGPKF
EPLHRDMEKG DEDWNEFNDI NKLIIRSPLR TEYKVAFPHL YNNRPRKVKL CVYHTPMVMY
IKTEDPDLPA FYYDPLIHPI SNSNNTNKEQ RKSNGYDDDG DDFVLPEGLE PLLNNSPLYT
DTTAPGISLL FAPRPFNMRS GRTRRAEDIP LVAEWFKEHC PPAYPVKVRV SYQKLLKCYL
LNELHHRPPK AQKKKHLFRS LAATKFFQST ELDWVEVGLQ VCRQGYNMLN LLIHRKNLNY
LHLDYNFNLK PVKTLTTKER KKSRFGNAFH LCREILRLTK LVVDANVQFR LGNVDAFQLA
DGLQYIFSHV GQLTGMYRYK YRLMRQIRMC KDLKHLIYYR FNTGPVGKGP GCGFWAPMWR
VWLFFLRGIV PLLERWLGNL LARQFEGRHS KGVAKTVTKQ RVESHFDLEL RAAVMHDVVD
AMPEGIKQNK ARTILQHLSE AWRCWKANIP WKVPGLPVAI ENMILRYVKS KADWWTNVAH
YNRERIRRGA TVDKTVCRKN LGRLTRLWLK AEQERQHNFQ KDGPYVTADE GIAIYSTTVN
WLESRKFSAI PFPPLSYKHD TKLLILALER LKESYSAAVK LNQQQREELG LIEQAYDNPH
EALMRIKRHL LTQHSFKEVG IEFMDLYSHL IPVYQIDPLE KITDAYLDQY LWYEGDKRHL
FPNWIKPADS EPPPLLVYKW CQGINNLQGI WDTSDGQCVV MLQTKFEKLF EKIDLTVLNS
LLRLVLDPKL ANYVTGKNNV VLSYKDMSYT NTYGLIRGLQ FASFVVQFYG LVLDLLLLGL
TRASEIAGPP QRPNEFMTYW DTKVETRHPI RLYSRYIDKV HIMFKFTHEE ARDLIQRHLT
ERPDPNNENM VGYNNKKCWP RDARMRLMKH DVNLGRSVFW DMKNRLPRSI TTLEWENGFV
SVYSKDNPNL LFSMCGFEVR VLPKIRMGQE AFSSTRDGVW NLQNEQTKER TAVAFLRADD
EHMKVFENRV RQILMSSGST TFTKIVNKWN TALIGLMTYF REATVHTQEL LDLLVKCENK
IQTRVKIGLN SKMPSRFPPV IFYTPKEIGG LGMLSMGHIL IPQSDLRYSN QTDVGVSHFR
SGMSHEEDQL IPNLYRYIQP WESEFIDSQR VWAEYALKRQ EAQAQNRRLT LEDLEDSWDR
GIPRINTLFQ KDRHTLAYDK GWRVRTDFKQ YQALKQNPFW WTHQRHDGKL WNLNNYRTDV
IQALGGVEGI LEHTLFKGTY FPTWEGLFWE KASGFEESMK YKKLTNAQRS GLNQIPNRRF
TLWWSPTINR ANVYVGFQVQ LDLTGIYMHG KIPTLKISLI QIFRAHLWQK IHESVVMDLC
QVLDQELEPL EIETVQKETI HPRKSYKMNS SCADVLLFAA HKWPMSKPSL IAESKDVFDQ
KASNKYWIDV QLRWGDYDSH DIERYTKAKF MDYTTDNMSI YPSPTGVIIG LDLAYNLHSA
FGNWFPGSKP LLAQAMNKIM KSNPALYVLR ERIRKGLQLY SSEPTEPYLS SQNYGEIFSN
QIIWFVDDTN VYRVTIHKTF EGNLTTKPIN GVIFIFNPRT GQLFLKIIHT SVWAGQKRLG
QLAKWKTAEE VAALVRSLPV EEQPKQVIVT RKGMLDPLEV HLLDFPNIVI KGSELQLPFQ
ACLKIEKFGD LILKATEPQM ALFNIYDDWL MTVSSYTAFQ RLILILRALH VNNEKAKMLL
KPDMSVVTEP NHIWPSLTDD QWMKVEVALR DLILSDYAKK NKVNTSALTQ SEIRDIILGA
EITPPSQQRQ QIAEIEKQAK EASQLTAVTT RTTNVHGDEL ISTTISPYEQ SAFGSKTDWR
VRAISATNLY LRVNHIYVNS DDIKETGYTY IMPKNILKKF ICIADLRTQI AGYLYGISPP
DNPQVKEIRC VVMVPQCGNH QQVQLPSSLP EHQFLDDLEP LGWIHTQPNE LPQLSPQDVT
FHTRVLENNK QWDAEKCIIL TCSFTPGSCS LTSYKLTQAG YEWGRLNKDT GSNPHGYLPT
HYEKVQMLLS DRFFGFYMVP ENGPWNYNFM GANHTVSINY SLTLGTPKEY YHQVHRPTHF
LQFSKMEEDG DLDRDDSFA
