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PRP8_DICDI
ID   PRP8_DICDI              Reviewed;        2327 AA.
AC   Q8T295; Q554T3;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 113.
DE   RecName: Full=Pre-mRNA-processing-splicing factor 8 homolog;
DE   AltName: Full=Splicing factor Prp8;
GN   Name=prpf8; Synonyms=prp8; ORFNames=DDB_G0274229;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Functions as a scaffold that mediates the ordered assembly of
CC       spliceosomal proteins and snRNAs. Required for the assembly of the
CC       U4/U6-U5 tri-snRNP complex. Functions as scaffold that positions
CC       spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA
CC       substrates, so that splicing can occur. Interacts with both the 5' and
CC       the 3' splice site. {ECO:0000250|UniProtKB:Q99PV0}.
CC   -!- SUBUNIT: Part of the U5 snRNP complex and of the U4/U6-U5 tri-snRNP
CC       complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000250}.
CC   -!- DOMAIN: The MPN (JAB/Mov34) domain has structural similarity with
CC       deubiquitinating enzymes, but lacks the residues that would bind the
CC       catalytic metal ion. {ECO:0000250}.
CC   -!- DOMAIN: Contains a region with structural similarity to reverse
CC       transcripase, presenting the classical thumb, fingers and palm
CC       architecture, but lacks enzyme activity, since the essential metal-
CC       binding residues are not conserved. {ECO:0000250}.
CC   -!- DOMAIN: Contains a region with structural similarity to type-2
CC       restriction endonucleases, but the residues that would bind catalytic
CC       metal ions in endonucleases are instead involved in hydrogen bonds that
CC       stabilize the protein structure. {ECO:0000250}.
CC   -!- DOMAIN: Contains a region with structural similarity to RNase H, but
CC       lacks RNase H activity. {ECO:0000250}.
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DR   EMBL; AAFI02000012; EAL70007.1; -; Genomic_DNA.
DR   RefSeq; XP_644240.1; XM_639148.1.
DR   AlphaFoldDB; Q8T295; -.
DR   SMR; Q8T295; -.
DR   STRING; 44689.DDB0233128; -.
DR   PaxDb; Q8T295; -.
DR   PRIDE; Q8T295; -.
DR   EnsemblProtists; EAL70007; EAL70007; DDB_G0274229.
DR   GeneID; 8619668; -.
DR   KEGG; ddi:DDB_G0274229; -.
DR   dictyBase; DDB_G0274229; prpf8.
DR   eggNOG; KOG1795; Eukaryota.
DR   HOGENOM; CLU_000380_3_0_1; -.
DR   InParanoid; Q8T295; -.
DR   OMA; VCMRREK; -.
DR   PhylomeDB; Q8T295; -.
DR   Reactome; R-DDI-72163; mRNA Splicing - Major Pathway.
DR   Reactome; R-DDI-72165; mRNA Splicing - Minor Pathway.
DR   PRO; PR:Q8T295; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR   GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR   GO; GO:0005682; C:U5 snRNP; IBA:GO_Central.
DR   GO; GO:0070122; F:isopeptidase activity; IEA:InterPro.
DR   GO; GO:0140492; F:metal-dependent deubiquitinase activity; IEA:InterPro.
DR   GO; GO:0097157; F:pre-mRNA intronic binding; IBA:GO_Central.
DR   GO; GO:0030619; F:U1 snRNA binding; IBA:GO_Central.
DR   GO; GO:0030620; F:U2 snRNA binding; IBA:GO_Central.
DR   GO; GO:0030623; F:U5 snRNA binding; IBA:GO_Central.
DR   GO; GO:0017070; F:U6 snRNA binding; IBA:GO_Central.
DR   GO; GO:0006397; P:mRNA processing; ISS:dictyBase.
DR   GO; GO:0008380; P:RNA splicing; ISS:dictyBase.
DR   GO; GO:0000244; P:spliceosomal tri-snRNP complex assembly; IBA:GO_Central.
DR   CDD; cd13838; RNase_H_like_Prp8_IV; 1.
DR   Gene3D; 1.20.80.40; -; 1.
DR   Gene3D; 3.30.420.230; -; 1.
DR   Gene3D; 3.30.43.40; -; 1.
DR   InterPro; IPR000555; JAMM/MPN+_dom.
DR   InterPro; IPR037518; MPN.
DR   InterPro; IPR012591; PRO8NT.
DR   InterPro; IPR012592; PROCN.
DR   InterPro; IPR012984; PROCT.
DR   InterPro; IPR027652; PRP8.
DR   InterPro; IPR021983; PRP8_domainIV.
DR   InterPro; IPR043173; Prp8_domainIV_fingers.
DR   InterPro; IPR043172; Prp8_domainIV_palm.
DR   InterPro; IPR019581; Prp8_U5-snRNA-bd.
DR   InterPro; IPR042516; Prp8_U5-snRNA-bd_sf.
DR   InterPro; IPR019580; Prp8_U6-snRNA-bd.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR019582; RRM_spliceosomal_PrP8.
DR   PANTHER; PTHR11140; PTHR11140; 1.
DR   Pfam; PF01398; JAB; 1.
DR   Pfam; PF08082; PRO8NT; 1.
DR   Pfam; PF08083; PROCN; 1.
DR   Pfam; PF08084; PROCT; 1.
DR   Pfam; PF12134; PRP8_domainIV; 1.
DR   Pfam; PF10598; RRM_4; 1.
DR   Pfam; PF10597; U5_2-snRNA_bdg; 1.
DR   Pfam; PF10596; U6-snRNA_bdg; 1.
DR   SMART; SM00232; JAB_MPN; 1.
DR   SUPFAM; SSF53098; SSF53098; 2.
DR   PROSITE; PS50249; MPN; 1.
PE   3: Inferred from homology;
KW   mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW   Ribonucleoprotein; RNA-binding; Spliceosome.
FT   CHAIN           1..2327
FT                   /note="Pre-mRNA-processing-splicing factor 8 homolog"
FT                   /id="PRO_0000327448"
FT   DOMAIN          2093..2223
FT                   /note="MPN"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01182"
FT   REGION          1..20
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          801..1292
FT                   /note="Reverse transcriptase homology domain"
FT   REGION          1293..1566
FT                   /note="Linker"
FT   REGION          1502..1515
FT                   /note="Important for branch point selection"
FT                   /evidence="ECO:0000250"
FT   REGION          1570..1740
FT                   /note="Restriction endonuclease homology domain"
FT   REGION          1657..2023
FT                   /note="Involved in interaction with pre-mRNA 5' splice
FT                   site"
FT                   /evidence="ECO:0000250"
FT   REGION          1755..2008
FT                   /note="RNase H homology domain"
SQ   SEQUENCE   2327 AA;  272196 MW;  F2B761E99EEF5EA9 CRC64;
     MDDTNSNINQ SNESQHLEEK AKKWIQLNNK KYSEKRKFGA VEIRKEDMPP EHLRKIIKDH
     GDMSNRRFRD DKRVYLGALK YMPHAILKLL ENIPMPWEQV KYVKVLYHLS GAITFVNEIP
     FVIEPIYIAQ WATMWVTMRR EKRDRTHFRR MKFPLFDDEE PPLDYSDNIL DNEVEDPIQM
     ELDENDDSEV IDWLYDSKPL VNTKFVNGSS YRKWRLNLPI MSTLFRLASP LLSDLTDSNY
     FYLFDDNSFF TSKALNMAIP GGPKFEPLFR DVDDDDEDWN EFNDINKVII RNKIRTEYKI
     AFPYLYNSRP RKVKTPTYHT PNNCYIKNDS PDLPGFYFGA ALNPIPSYKT SGNKNEQSEY
     GTEDDEFQLP EEIETILSKT EIEHDNLANG IQLYWAPRPF SLRSGTTRRA EDIPLVKSWY
     KEHCPSEHPV KVRVSYQKLL KCHVLNKLHH RKPKAQTKRN LFKSLKATKF FQSTEIDWVE
     AGLQVCRQGY NMLNLLIHRK NLNYLHLDYN FYLKPIKTLT TKERKKSRFG NAFHLCREIL
     RLTKLVVDVH VKFRLGDADA FQLADAIQYL FSHLGLLTGM YKYKYRLMRQ IRMCKDLKHL
     IYYRFNTGAV GKGPGCGFWA PMWRVWLFFL RGIVPLLERW LGNLLARQFE GRQTKGMAKT
     VTKQRVESHF DYELRAAVMH DILDMMPEGI KANKSRIILQ HLSEAWRCWK SNIPWKVPGL
     PIPIENMILR YVKSKADWWT NIAHYNRERI KRGATIDKTA SKKNLGRLTR LWLKAEQERQ
     HNYLKDGPYV SAEEAVAIYT TTVHWLEKRR FSAIPFPQTS YKHDIKILTL ALERLKEAYS
     VKSRLNQSQR EELSLVEQAY DNPHDALARI KRHLLTQRTF KEVGIEFMDM YTHLVPIYDV
     DPFEKITDAY LDQYLWYEAD KRQLFPNWVK PSDNEPPPVL IHKWCQGINN LDQVWETSQG
     ECVVLLETQF SKVYEKMDLT LMNRLLRLIV DQNIADYMSG KNNVVINYKD MNHTNSYGLI
     RGLQFASFIF QYYGLVLDLL VLGLERASAL AGPPNLPNSF LTFPSVQTET AHPIRLYSRY
     VDRIHVLYKF TADEARKLIQ KYMSEHPDPN NENVVGYNNK KCWPRDCRMR LMKHDVNLGR
     AVFWQIKNRL PRSLTTIDWE DSFVSVYSKD NPNLLMNMAG FDIRILPKCR TPLDQLAPKD
     AVWSLQNVNT KERTAQAFLR VDTESQERFE NRIRMILMAS GSTTFTKIVN KWNTALIGLM
     TYYREAVVTT REMLDILVRC ENKIQTRVKI GLNSKMPNRF PPVVFYTPKE LGGLGMLSMG
     HVLIPQSDLK YSKQTDTGIT HFTSGMSHDE DQLIPNLYRY IQPWEQEIKD SQRVWAEYAI
     KYEEAKSQNK NLTLEDLEDS WDRGIPRINT LFQKSRHTLA YDKGWRVRTD WKQYQVLKNN
     PFWWTNQRHD GKLWNLNNYR TDIIQALGGV EGILEHTLFK GTYFPTWEGL FWEKASGFEE
     SMKYKKLTHA QRSGLNQIPN RRFTLWWSPT INRKNVYVGF QVQLDLTGIF MHGKIPTLKI
     SLIQIFRAHL WQKIHESLVM DLCQVFDQEL DNLEISVVNK EAIHPRKSYK MNSSCADILL
     RATHKWQVSR PSLLNDNRDT YDNTTTQYWL DVQLKWGDFD SHDIERYSRA KFLDYTTDSM
     SLYPSPTGCL IGLDLAYNIY SSFGNWFLGV KPLVQKAMAK ILKSNPALYV LRERIRKGLQ
     LYSSEPTEPY LSSQNFGELF SNKIMWFVDD SNVYRVTIHK TFEGNLTTKP INGAIFIFNP
     RTGQLFLKII HTDVWLGQKR LGQLAKWKTA EEVAALIRSL PVEEQPKQII ATRKGMMDPL
     EVHLLDFPNI VIQGSELQLP FQACLKVEKF GDLILKATEP KMVLFNIYDD WLSTIHSYTA
     FLRLILILRA LHVNLERTKI ILKPNKNVIT QPHHIWPTLT EQEWLTVEGS LKDLILADFG
     KRNNVNVASL TQSEIRDIIL GMEISAPSQQ REDQIAEIEK QKTEASHLTA VTVRSTNIHG
     EEIITTATSP HEQKVFSSKT DWRVRAISAT NLHLRTNQIY VNSDNAKETG GFTYVFPKNI
     LKKFITIADL RTQIMGYCYG ISPPDNPSVK EIRCIVMPPQ WGTPVHVTVP NQLPEHEYLK
     DLEPLGWIHT QPTELPQLSP QDVITHSKIM SDNKSWDGEK TVIISVSVAW PCTLTAYHLT
     PSGFEWGKNN KDSLNYQGYQ PQFYEKVQML LSDRFLGFYM VPDRGSWNYN FMGVKHSTNM
     TYGLKLDYPK NFYDESHRPA HFQNWTQMAP SANDDEENQP ENENLFE
 
 
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