ID   PRPD_DICDI              Reviewed;         492 AA.
AC   Q54IJ0; P90535;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Probable 2-methylcitrate dehydratase {ECO:0000250|UniProtKB:P77243};
DE            Short=2-MC dehydratase {ECO:0000250|UniProtKB:P77243};
DE            EC=4.2.1.79 {ECO:0000250|UniProtKB:P77243};
DE   AltName: Full=(2S,3S)-2-methylcitrate dehydratase {ECO:0000250|UniProtKB:P77243};
GN   Name=prpD; ORFNames=DDB_G0288681;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 163-492.
RC   STRAIN=AX4;
RA   Iranfar N., Loomis W.F.;
RL   Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the stereospecific dehydration of (2S,3S)-2-
CC       methylcitrate (2-MC) to yield the cis isomer of 2-methyl-aconitate.
CC       {ECO:0000250|UniProtKB:P77243}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2S,3S)-2-methylcitrate = 2-methyl-cis-aconitate + H2O;
CC         Xref=Rhea:RHEA:17725, ChEBI:CHEBI:15377, ChEBI:CHEBI:57872,
CC         ChEBI:CHEBI:58853; EC=4.2.1.79;
CC         Evidence={ECO:0000250|UniProtKB:P77243};
CC   -!- PATHWAY: Organic acid metabolism; propanoate degradation.
CC       {ECO:0000250|UniProtKB:P77243}.
CC   -!- SIMILARITY: Belongs to the PrpD family. {ECO:0000305}.
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DR   EMBL; AAFI02000120; EAL63072.1; -; Genomic_DNA.
DR   EMBL; U83088; AAB40931.1; -; mRNA.
DR   RefSeq; XP_636596.1; XM_631504.1.
DR   AlphaFoldDB; Q54IJ0; -.
DR   SMR; Q54IJ0; -.
DR   STRING; 44689.DDB0191366; -.
DR   PaxDb; Q54IJ0; -.
DR   EnsemblProtists; EAL63072; EAL63072; DDB_G0288681.
DR   GeneID; 8626771; -.
DR   KEGG; ddi:DDB_G0288681; -.
DR   dictyBase; DDB_G0288681; prpD.
DR   eggNOG; ENOG502QPZI; Eukaryota.
DR   HOGENOM; CLU_021803_1_0_1; -.
DR   InParanoid; Q54IJ0; -.
DR   OMA; ECTKHLG; -.
DR   PhylomeDB; Q54IJ0; -.
DR   UniPathway; UPA00946; -.
DR   PRO; PR:Q54IJ0; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:InterPro.
DR   GO; GO:0047547; F:2-methylcitrate dehydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019679; P:propionate metabolic process, methylcitrate cycle; IEA:InterPro.
DR   Gene3D; 1.10.4100.10; -; 1.
DR   Gene3D; 3.30.1330.120; -; 1.
DR   InterPro; IPR012705; 2Me_IsoCit_deHydtase_PrpD.
DR   InterPro; IPR036148; MmgE/PrpD_sf.
DR   InterPro; IPR042183; MmgE/PrpD_sf_1.
DR   InterPro; IPR042188; MmgE/PrpD_sf_2.
DR   InterPro; IPR005656; MmgE_PrpD.
DR   InterPro; IPR045337; MmgE_PrpD_C.
DR   InterPro; IPR045336; MmgE_PrpD_N.
DR   PANTHER; PTHR16943; PTHR16943; 1.
DR   Pfam; PF03972; MmgE_PrpD; 1.
DR   Pfam; PF19305; MmgE_PrpD_C; 1.
DR   SUPFAM; SSF103378; SSF103378; 1.
DR   TIGRFAMs; TIGR02330; prpD; 1.
PE   2: Evidence at transcript level;
KW   Lyase; Reference proteome.
FT   CHAIN           1..492
FT                   /note="Probable 2-methylcitrate dehydratase"
FT                   /id="PRO_0000328266"
SQ   SEQUENCE   492 AA;  54984 MW;  CE1B4F05C0C9821A CRC64;
     MNNNIEQKWD KVLEDIVDYV IGFDVEGNSK EAMETAFLAL FDTIGCGFLG LKSTGCTKLM
     GPTIPDTRIP GGCRVPGTDY QLNPIEAAFN IGCMNRWLDF NDCWLANEWG HPSDNISAII
     AAAEYISNDN ISKSKKPLLM KDILCAMVKC YEIQGVLALD NSFNKIGLDH VILVKIASTA
     VTCHLLGGSR IQILNALSNA FIDGHPLRTY RHSPNTGSRK GWAAGDAASR AVQLSLMAMA
     GEMGYPTCLS APKWGFYHVY MSDKPFQFNQ RSNYSTYVME NILFKVLYPA EFHGQTAIEC
     AIQLHPIFQD KGGLNSVESI TIETTEPAIR IIDKRGILRN PSDRDHCLQF IVSVALIFGE
     LKSSHYEDQV AIKDTRIDLL REKITCIENL QFSLDYLNPD KRSIANSITI LFKDGETHKH
     QIDYPIGHRN RRSEAIPLLQ QKFLNSLNST LFTSSSIDQI KNLYENPSKF NNLSILDFMS
     IFSLSIASTP IS