PRPE_BACAN
ID PRPE_BACAN Reviewed; 246 AA.
AC Q81TQ0; Q6I1Y4; Q6KVS4;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 118.
DE RecName: Full=Bis(5'-nucleosyl)-tetraphosphatase PrpE [asymmetrical] {ECO:0000255|HAMAP-Rule:MF_01443};
DE EC=3.6.1.17 {ECO:0000255|HAMAP-Rule:MF_01443};
DE AltName: Full=Ap4A hydrolase {ECO:0000255|HAMAP-Rule:MF_01443};
DE AltName: Full=Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase {ECO:0000255|HAMAP-Rule:MF_01443};
DE Short=Diadenosine tetraphosphatase {ECO:0000255|HAMAP-Rule:MF_01443};
GN Name=prpE {ECO:0000255|HAMAP-Rule:MF_01443};
GN OrderedLocusNames=BA_1217, GBAA_1217, BAS1124;
OS Bacillus anthracis.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=1392;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ames / isolate Porton;
RX PubMed=12721629; DOI=10.1038/nature01586;
RA Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T.,
RA Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R.,
RA Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M.,
RA Kolonay J.F., Beanan M.J., Dodson R.J., Brinkac L.M., Gwinn M.L.,
RA DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C.,
RA Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y.,
RA Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M.,
RA Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E.,
RA White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M.,
RA Hanna P.C., Kolstoe A.-B., Fraser C.M.;
RT "The genome sequence of Bacillus anthracis Ames and comparison to closely
RT related bacteria.";
RL Nature 423:81-86(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ames ancestor;
RX PubMed=18952800; DOI=10.1128/jb.01347-08;
RA Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D.,
RA Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.;
RT "The complete genome sequence of Bacillus anthracis Ames 'Ancestor'.";
RL J. Bacteriol. 191:445-446(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Sterne;
RA Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K.,
RA Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R.,
RA Richardson P., Rubin E., Tice H.;
RT "Complete genome sequence of Bacillus anthracis Sterne.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Asymmetrically hydrolyzes Ap4p to yield AMP and ATP.
CC {ECO:0000255|HAMAP-Rule:MF_01443}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + P(1),P(4)-bis(5'-guanosyl) tetraphosphate = GMP + GTP +
CC 2 H(+); Xref=Rhea:RHEA:22484, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:37565, ChEBI:CHEBI:57553, ChEBI:CHEBI:58115; EC=3.6.1.17;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01443};
CC -!- COFACTOR:
CC Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01443};
CC -!- SIMILARITY: Belongs to the PrpE family. {ECO:0000255|HAMAP-
CC Rule:MF_01443}.
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DR EMBL; AE016879; AAP25177.1; -; Genomic_DNA.
DR EMBL; AE017334; AAT30303.1; -; Genomic_DNA.
DR EMBL; AE017225; AAT53447.1; -; Genomic_DNA.
DR RefSeq; NP_843691.1; NC_003997.3.
DR RefSeq; WP_000872719.1; NZ_WXXJ01000044.1.
DR RefSeq; YP_027396.1; NC_005945.1.
DR AlphaFoldDB; Q81TQ0; -.
DR SMR; Q81TQ0; -.
DR IntAct; Q81TQ0; 1.
DR STRING; 261594.GBAA_1217; -.
DR DNASU; 1087409; -.
DR EnsemblBacteria; AAP25177; AAP25177; BA_1217.
DR EnsemblBacteria; AAT30303; AAT30303; GBAA_1217.
DR GeneID; 45021219; -.
DR KEGG; ban:BA_1217; -.
DR KEGG; bar:GBAA_1217; -.
DR KEGG; bat:BAS1124; -.
DR PATRIC; fig|198094.11.peg.1193; -.
DR eggNOG; COG0639; Bacteria.
DR HOGENOM; CLU_023125_3_0_9; -.
DR OMA; CNKLYRY; -.
DR Proteomes; UP000000427; Chromosome.
DR Proteomes; UP000000594; Chromosome.
DR GO; GO:0004081; F:bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR CDD; cd07423; MPP_Prp_like; 1.
DR Gene3D; 3.60.21.10; -; 1.
DR HAMAP; MF_01443; PrpE; 1.
DR InterPro; IPR023937; Bis(5'-nucleosyl)-tetraP_PrpE.
DR InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR InterPro; IPR029052; Metallo-depent_PP-like.
DR InterPro; IPR041780; MPP_PrpE-like.
DR Pfam; PF00149; Metallophos; 1.
DR SUPFAM; SSF56300; SSF56300; 1.
PE 3: Inferred from homology;
KW GTP-binding; Hydrolase; Nickel; Nucleotide-binding; Reference proteome.
FT CHAIN 1..246
FT /note="Bis(5'-nucleosyl)-tetraphosphatase PrpE
FT [asymmetrical]"
FT /id="PRO_0000297695"
SQ SEQUENCE 246 AA; 28269 MW; 6868D3223207DCA8 CRC64;
MKYDIIGDIH GCLQEFQNLT EKLGYNWSSG LPVHPDQRKL AFVGDITDRG PHSLRMIEIV
WELVIHKKVA YYAPGNHCNK LYRFFLGRNV TIAHGLETTV AEYEALPSHK QNMIKEKFIT
LYEQSPLYHI LDEKRLLVCH AGIRQDYIGR QDKKVQTFVL YGDITGEKHA DGSPVRRDWA
KEYKGTTWIV YGHTPVKEPR FVNHTVNIDT GAVFGGRLTA LRYPEMETVS VPSSLPFVPE
KFRPIS
