ID   ATG32_ASHGO             Reviewed;         452 AA.
AC   Q753M9;
DT   05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Autophagy-related protein 32;
GN   Name=ATG32; OrderedLocusNames=AFR283W;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Mitophagy-specific receptor that recruits the autophagic
CC       machinery to mitochondria and regulates selective degradation of
CC       mitochondria. Mitophagy contributes to regulate mitochondrial quantity
CC       and quality by eliminating the mitochondria to a basal level to fulfill
CC       cellular energy requirements and preventing excess ROS production.
CC       Recruits ATG11 to the surface of mitochondria. Promotes also autophagy-
CC       dependent peroxisome degradation (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}. Vacuole membrane
CC       {ECO:0000250}; Single-pass membrane protein {ECO:0000250}.
CC       Preautophagosomal structure membrane {ECO:0000250}; Single-pass
CC       membrane protein {ECO:0000250}. Note=Is recruited to the
CC       preautophagosomal structure during mitophagy and imported into the
CC       vacuole along with mitochondria during starvation. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ATG32 family. {ECO:0000305}.
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DR   EMBL; AE016819; AAS53654.1; -; Genomic_DNA.
DR   RefSeq; NP_985830.1; NM_211185.2.
DR   AlphaFoldDB; Q753M9; -.
DR   SMR; Q753M9; -.
DR   STRING; 33169.AAS53654; -.
DR   PRIDE; Q753M9; -.
DR   EnsemblFungi; AAS53654; AAS53654; AGOS_AFR283W.
DR   GeneID; 4622093; -.
DR   KEGG; ago:AGOS_AFR283W; -.
DR   eggNOG; ENOG502QY5V; Eukaryota.
DR   HOGENOM; CLU_039418_0_0_1; -.
DR   InParanoid; Q753M9; -.
DR   OMA; IPICQPG; -.
DR   Proteomes; UP000000591; Chromosome VI.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Autophagy; Membrane; Mitochondrion; Mitochondrion outer membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Vacuole.
FT   CHAIN           1..452
FT                   /note="Autophagy-related protein 32"
FT                   /id="PRO_0000399756"
FT   TRANSMEM        336..356
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..28
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   452 AA;  50405 MW;  677AFEFE4EB9A648 CRC64;
     MSTKSQVTRR VRTSIATPED GVHGNNQHKG ILDPHLSVLE MLDRQDGDGA GQVEEGAVMT
     VGKRRVERSL HHSISESWQA IKRSDYSFLS GTHEVGAMHS SVGILSSSDT SEEEAEMRPS
     AHGTVHLGSS LASPMRQLLV EEDNSCAEED DCQTVTISMP SSSTSLVMPK LSLSQRLGEP
     QLLLVGQPAR KFWLTIPKCY QKLFDVKNLG MVTRWDVGQR YLAVMVVFHD IAQAPELLDG
     LCEKAPCPTV IPVCQKGQKS TLAALLKRYT ARKCIRVYCS PIIMSNHHEK HRLLKHLHNL
     CNESESGYET ELTVKSKKQH RRPRKKDAGP VALRHWAIWT ASFTIGIGIG CCISLMATTR
     FTFFSSAPLP LTAVIPAQIP SSVASDKPPH RLVPHFYMLC KTTIRQLGTS LRLFFFEKFE
     SRTWVHIFGM DLHSDDPLAS LGRLMPLDFI ML