PRQFV_APLCA
ID PRQFV_APLCA Reviewed; 862 AA.
AC Q86MA7;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 30.
DE RecName: Full=Protein PRQFV-amide;
DE Contains:
DE RecName: Full=AREFV-amide;
DE Contains:
DE RecName: Full=PRQFV-amide;
DE Short=PRQFVa;
DE Contains:
DE RecName: Full=VRDFV-amide;
DE Contains:
DE RecName: Full=VREFV-amide;
DE Contains:
DE RecName: Full=IREFV-amide;
DE Flags: Precursor;
OS Aplysia californica (California sea hare).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC Heterobranchia; Euthyneura; Tectipleura; Aplysiida; Aplysioidea;
OC Aplysiidae; Aplysia.
OX NCBI_TaxID=6500;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, PROTEOLYTIC
RP PROCESSING, AMIDATION AT VAL-86; VAL-156; VAL-182; VAL-190; VAL-198;
RP VAL-206; VAL-214; VAL-222; VAL-230; VAL-238; VAL-246; VAL-266; VAL-307;
RP VAL-326; VAL-345; VAL-364; VAL-383; VAL-402; VAL-421; VAL-440; VAL-458;
RP VAL-477; VAL-496; VAL-514; VAL-533; VAL-552; VAL-571; VAL-590; VAL-609;
RP VAL-628; VAL-647; VAL-666; VAL-684; VAL-702; VAL-721; VAL-764 AND VAL-819,
RP AND MASS SPECTROMETRY.
RC TISSUE=CNS;
RX PubMed=12612009; DOI=10.1152/jn.00014.2003;
RA Furukawa Y., Nakamaru K., Sasaki K., Fujisawa Y., Minakata H., Ohta S.,
RA Morishita F., Matsushima O., Li L., Alexeeva V., Ellis T.A., Dembrow N.C.,
RA Jing J., Sweedler J.V., Weiss K.R., Vilim F.S.;
RT "PRQFVamide, a novel pentapeptide identified from the CNS and gut of
RT Aplysia.";
RL J. Neurophysiol. 89:3114-3127(2003).
CC -!- FUNCTION: PRQFV-amide may act as a modulator within the feeding system
CC as well as in other systems of Aplysia. {ECO:0000269|PubMed:12612009}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Expressed abundantly in the abdominal ganglion,
CC much less in the pedal and cerebral ganglia, and rarely in the buccal
CC and pleural ganglia. {ECO:0000269|PubMed:12612009}.
CC -!- MASS SPECTROMETRY: [AREFV-amide]: Mass=619.33; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:12612009};
CC -!- MASS SPECTROMETRY: [PRQFV-amide]: Mass=644.36; Method=MALDI;
CC Note=PRQFV-amide.; Evidence={ECO:0000269|PubMed:12612009};
CC -!- MASS SPECTROMETRY: [VRDFV-amide]: Mass=633.34; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:12612009};
CC -!- MASS SPECTROMETRY: [VREFV-amide]: Mass=647.36; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:12612009};
CC -!- MASS SPECTROMETRY: [IREFV-amide]: Mass=661.38; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:12612009};
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DR EMBL; AY231295; AAO73964.1; -; mRNA.
DR RefSeq; NP_001191529.1; NM_001204600.1.
DR AlphaFoldDB; Q86MA7; -.
DR GeneID; 100533301; -.
DR OrthoDB; 1737874at2759; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Amidation; Neuropeptide; Secreted; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT PROPEP 21..79
FT /evidence="ECO:0000255"
FT /id="PRO_0000309785"
FT PEPTIDE 82..86
FT /note="AREFV-amide"
FT /id="PRO_0000309748"
FT PROPEP 90..149
FT /id="PRO_0000309786"
FT PEPTIDE 152..156
FT /note="PRQFV-amide"
FT /id="PRO_0000309749"
FT PROPEP 160..175
FT /id="PRO_0000309787"
FT PEPTIDE 178..182
FT /note="PRQFV-amide"
FT /id="PRO_0000309750"
FT PEPTIDE 186..190
FT /note="PRQFV-amide"
FT /id="PRO_0000309751"
FT PEPTIDE 194..198
FT /note="PRQFV-amide"
FT /id="PRO_0000309752"
FT PEPTIDE 202..206
FT /note="PRQFV-amide"
FT /id="PRO_0000309753"
FT PEPTIDE 210..214
FT /note="PRQFV-amide"
FT /id="PRO_0000309754"
FT PEPTIDE 218..222
FT /note="PRQFV-amide"
FT /id="PRO_0000309755"
FT PEPTIDE 226..230
FT /note="PRQFV-amide"
FT /id="PRO_0000309756"
FT PEPTIDE 234..238
FT /note="PRQFV-amide"
FT /id="PRO_0000309757"
FT PEPTIDE 242..246
FT /note="PRQFV-amide"
FT /id="PRO_0000309758"
FT PROPEP 250..259
FT /id="PRO_0000309788"
FT PEPTIDE 262..266
FT /note="VRDFV-amide"
FT /id="PRO_0000309759"
FT PROPEP 270..300
FT /id="PRO_0000309789"
FT PEPTIDE 303..307
FT /note="PRQFV-amide"
FT /id="PRO_0000309760"
FT PROPEP 311..319
FT /id="PRO_0000309790"
FT PEPTIDE 322..326
FT /note="PRQFV-amide"
FT /id="PRO_0000309761"
FT PROPEP 330..338
FT /id="PRO_0000309791"
FT PEPTIDE 341..345
FT /note="PRQFV-amide"
FT /id="PRO_0000309762"
FT PROPEP 349..357
FT /id="PRO_0000309792"
FT PEPTIDE 360..364
FT /note="PRQFV-amide"
FT /id="PRO_0000309763"
FT PROPEP 368..376
FT /id="PRO_0000309793"
FT PEPTIDE 379..383
FT /note="PRQFV-amide"
FT /id="PRO_0000309764"
FT PROPEP 387..395
FT /id="PRO_0000309794"
FT PEPTIDE 398..402
FT /note="PRQFV-amide"
FT /id="PRO_0000309765"
FT PROPEP 406..414
FT /id="PRO_0000309795"
FT PEPTIDE 417..421
FT /note="PRQFV-amide"
FT /id="PRO_0000309766"
FT PROPEP 425..433
FT /id="PRO_0000309796"
FT PEPTIDE 436..440
FT /note="PRQFV-amide"
FT /id="PRO_0000309767"
FT PROPEP 444..451
FT /id="PRO_0000309797"
FT PEPTIDE 454..458
FT /note="PRQFV-amide"
FT /id="PRO_0000309768"
FT PROPEP 462..470
FT /id="PRO_0000309798"
FT PEPTIDE 473..477
FT /note="PRQFV-amide"
FT /id="PRO_0000309769"
FT PROPEP 481..489
FT /id="PRO_0000309799"
FT PEPTIDE 492..496
FT /note="PRQFV-amide"
FT /id="PRO_0000309770"
FT PROPEP 500..507
FT /id="PRO_0000309800"
FT PEPTIDE 510..514
FT /note="PRQFV-amide"
FT /id="PRO_0000309771"
FT PROPEP 518..526
FT /id="PRO_0000309801"
FT PEPTIDE 529..533
FT /note="PRQFV-amide"
FT /id="PRO_0000309772"
FT PROPEP 537..545
FT /id="PRO_0000309802"
FT PEPTIDE 548..552
FT /note="PRQFV-amide"
FT /id="PRO_0000309773"
FT PROPEP 556..564
FT /id="PRO_0000309803"
FT PEPTIDE 567..571
FT /note="PRQFV-amide"
FT /id="PRO_0000309774"
FT PROPEP 575..583
FT /id="PRO_0000309804"
FT PEPTIDE 586..590
FT /note="PRQFV-amide"
FT /id="PRO_0000309775"
FT PROPEP 594..602
FT /id="PRO_0000309805"
FT PEPTIDE 605..609
FT /note="PRQFV-amide"
FT /id="PRO_0000309776"
FT PROPEP 613..621
FT /id="PRO_0000309806"
FT PEPTIDE 624..628
FT /note="PRQFV-amide"
FT /id="PRO_0000309777"
FT PROPEP 632..640
FT /id="PRO_0000309807"
FT PEPTIDE 643..647
FT /note="PRQFV-amide"
FT /id="PRO_0000309778"
FT PROPEP 651..659
FT /id="PRO_0000309808"
FT PEPTIDE 662..666
FT /note="PRQFV-amide"
FT /id="PRO_0000309779"
FT PROPEP 670..677
FT /id="PRO_0000309809"
FT PEPTIDE 680..684
FT /note="PRQFV-amide"
FT /id="PRO_0000309780"
FT PROPEP 688..695
FT /id="PRO_0000309810"
FT PEPTIDE 698..702
FT /note="PRQFV-amide"
FT /id="PRO_0000309781"
FT PROPEP 706..714
FT /id="PRO_0000309811"
FT PEPTIDE 717..721
FT /note="PRQFV-amide"
FT /id="PRO_0000309782"
FT PROPEP 724..757
FT /id="PRO_0000309812"
FT PEPTIDE 760..764
FT /note="VREFV-amide"
FT /id="PRO_0000309783"
FT PROPEP 768..812
FT /id="PRO_0000309813"
FT PEPTIDE 815..819
FT /note="IREFV-amide"
FT /id="PRO_0000309784"
FT PROPEP 823..862
FT /id="PRO_0000309814"
FT REGION 32..68
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 424..528
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 549..634
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 44..68
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 86
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 156
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 182
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 190
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 198
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 206
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 214
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 222
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 230
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 238
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 246
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 266
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 307
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 326
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 345
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 364
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 383
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 402
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 421
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 440
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 458
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 477
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 496
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 514
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 533
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 552
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 571
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 590
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 609
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 628
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 647
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 666
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 684
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 702
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 721
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 764
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
FT MOD_RES 819
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:12612009"
SQ SEQUENCE 862 AA; 101508 MW; 90870C1CD7D4F208 CRC64;
MSSQLLICSV FVLFTFGPNS FPSCLAQEQA GNSDATQLSA DAKAPESAKD KSGDVQNDGT
KSVRSKRDLE IDFGSGDVQK RAREFVGKRA APPVFQTPLV QDKISGFIPS ETESPVIGEF
AFPGSVFMDD EEALGAEEEP MDDEDLEFYK RPRQFVGKRG IDDYLLQEKL KDFIEKRPRQ
FVGKRPRQFV GKRPRQFVGK RPRQFVGKRP RQFVGKRPRQ FVGKRPRQFV GKRPRQFVGK
RPRQFVGKRE ADPSFLFEDK RVRDFVGKRS LDFLGGANWY NPYDVTMEPQ SEGSDLQGFS
KRPRQFVGKR DAFDMFEFSK RPRQFVGKRD QDEMFDFSKR PRQFVGKRDL QEFFDLSKRP
RQFVGKREFD DEIDFSKRPR QFVGKRENDD DFDLSKRPRQ FVGKRENDDE FDLSKRPRQF
VGKRENDDEL EFSKRPRQFV GKREDDEIDF SKRPRQFVGK RENDGEIDFS KRPRQFVGKR
ENDDEIDFSK RPRQFVGKRE DGEIDFSKRP RQFVGKRENN DDLDFSKRPR QFVGKREVDD
EIDFSKRPRQ FVGKRENDDD LDFSKRPRQF VGKRENDDDL EFSKRPRQFV GKRENDPLLD
FSKRPRQFVG KRENDDDLDF SKRPRQFVGK RENDPLIDFS KRPRQFVGKR ESDGDFELSK
RPRQFVGKRD VDGPGLSKRP RQFVGKREDY DIDFAKRPRQ FVGKRGNEDE FEMSKRPRQF
VGKRNFEELD QDFLRHMHDI LDKRIPQFVS LPSLTAAKRV REFVGKRSDA AFLETLRHLR
DYVGGQDEQN VSEFSYQHPY PSDLNDVGLI QQKRIREFVG KRGGDVDDIN TTYRLGDFVS
QPMSFVEEPS WLCRQLNAFG IS
