PRR12_MOUSE
ID PRR12_MOUSE Reviewed; 2035 AA.
AC E9PYL2; Q69ZN8;
DT 17-FEB-2016, integrated into UniProtKB/Swiss-Prot.
DT 05-APR-2011, sequence version 1.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=Proline-rich protein 12;
GN Name=Prr12 {ECO:0000312|MGI:MGI:2679002}; Synonyms=Kiaa1205;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE OF 188-2035.
RC TISSUE=Embryonic tail {ECO:0000312|EMBL:BAD32408.1};
RX PubMed=15368895; DOI=10.1093/dnares/11.3.205;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H.,
RA Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: IV.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 11:205-218(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1555 AND SER-1562, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-648, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-330; SER-338; SER-648;
RP SER-859; SER-1128; SER-1372; SER-1373; THR-1555 AND SER-1562, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6]
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND SUBCELLULAR LOCATION.
RX PubMed=26163108; DOI=10.1007/s10048-015-0452-2;
RA Cordova-Fletes C., Dominguez M.G., Delint-Ramirez I.,
RA Martinez-Rodriguez H.G., Rivas-Estilla A.M., Barros-Nunez P.,
RA Ortiz-Lopez R., Neira V.A.;
RT "A de novo t(10;19)(q22.3;q13.33) leads to ZMIZ1/PRR12 reciprocal fusion
RT transcripts in a girl with intellectual disability and neuropsychiatric
RT alterations.";
RL Neurogenetics 16:287-298(2015).
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:26163108}.
CC Postsynaptic density {ECO:0000269|PubMed:26163108}. Synapse,
CC synaptosome {ECO:0000269|PubMed:26163108}. Note=A smaller form of
CC approximately 150 kDa has been found in perisynapse, synaptosomes and
CC postsynaptic density in 15 dpc, P1 and adult brains.
CC {ECO:0000269|PubMed:26163108}.
CC -!- TISSUE SPECIFICITY: Expressed in brain. {ECO:0000269|PubMed:26163108}.
CC -!- DEVELOPMENTAL STAGE: Strongest expression found in embryonic day 15 dpc
CC compared to postnatal day P1 and adult brain.
CC {ECO:0000269|PubMed:26163108}.
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DR EMBL; AC126256; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK173130; BAD32408.1; -; mRNA.
DR CCDS; CCDS52241.1; -.
DR RefSeq; NP_778187.2; NM_175022.2.
DR AlphaFoldDB; E9PYL2; -.
DR IntAct; E9PYL2; 1.
DR MINT; E9PYL2; -.
DR STRING; 10090.ENSMUSP00000054702; -.
DR iPTMnet; E9PYL2; -.
DR PhosphoSitePlus; E9PYL2; -.
DR EPD; E9PYL2; -.
DR jPOST; E9PYL2; -.
DR MaxQB; E9PYL2; -.
DR PaxDb; E9PYL2; -.
DR PeptideAtlas; E9PYL2; -.
DR PRIDE; E9PYL2; -.
DR ProteomicsDB; 291798; -.
DR Antibodypedia; 32065; 14 antibodies from 8 providers.
DR Ensembl; ENSMUST00000057293; ENSMUSP00000054702; ENSMUSG00000046574.
DR GeneID; 233210; -.
DR KEGG; mmu:233210; -.
DR UCSC; uc009gsu.1; mouse.
DR CTD; 57479; -.
DR MGI; MGI:2679002; Prr12.
DR VEuPathDB; HostDB:ENSMUSG00000046574; -.
DR eggNOG; KOG4805; Eukaryota.
DR GeneTree; ENSGT00440000037417; -.
DR HOGENOM; CLU_000708_0_0_1; -.
DR InParanoid; E9PYL2; -.
DR OMA; FPEMEDM; -.
DR OrthoDB; 52008at2759; -.
DR TreeFam; TF333141; -.
DR BioGRID-ORCS; 233210; 7 hits in 73 CRISPR screens.
DR ChiTaRS; Prr12; mouse.
DR PRO; PR:E9PYL2; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; E9PYL2; protein.
DR Bgee; ENSMUSG00000046574; Expressed in dorsal pancreas and 225 other tissues.
DR Genevisible; E9PYL2; MM.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0043005; C:neuron projection; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0014069; C:postsynaptic density; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR InterPro; IPR025451; DUF4211.
DR Pfam; PF13926; DUF4211; 1.
PE 1: Evidence at protein level;
KW Acetylation; DNA-binding; Nucleus; Phosphoprotein; Reference proteome;
KW Synapse; Synaptosome.
FT CHAIN 1..2035
FT /note="Proline-rich protein 12"
FT /id="PRO_0000435433"
FT REGION 210..280
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 292..311
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 329..584
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 645..685
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 755..844
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 851..870
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 879..920
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 946..1061
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1112..1244
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1288..1355
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1367..1567
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1662..1839
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 222..242
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 244..267
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 473..490
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 491..505
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 520..536
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 787..802
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 827..843
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1029..1049
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1189..1210
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1293..1328
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1417..1432
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1442..1533
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1709..1727
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1728..1763
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1764..1782
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1784..1803
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 330
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 338
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 648
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19144319,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 735
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9ULL5"
FT MOD_RES 859
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1070
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9ULL5"
FT MOD_RES 1128
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1214
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q9ULL5"
FT MOD_RES 1295
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9ULL5"
FT MOD_RES 1299
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9ULL5"
FT MOD_RES 1372
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1373
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1378
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9ULL5"
FT MOD_RES 1555
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 1562
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 1699
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9ULL5"
FT MOD_RES 1924
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9ULL5"
SQ SEQUENCE 2035 AA; 211876 MW; E19BEE293EC8630A CRC64;
MDRNYPSAGF GDPLGAGAGW SYERSAKASL VYGSSRTSHP ETDILHRQAY AAPHPLQSYA
TNHHPAGLSG LFDTGLHHAG SAGPDASVMN LISALESRGP QPGPSASSLL SQFRSPSWQT
AMHTPGPTEL FISGALPGSS TFPSSSALSA YQHPASFGSR PFPVPSSLSL QDPPFSPPAN
GLLSPHDVLH LKPSQAPTVP SSLGFERLAG GGVLGPAGLG PAQTPPYRPG PPDPPPPPRH
LPTQFNLLAS SSAATAAEPS SPQLYNFSGA APGPPPERAL PRQDTVIKHY QRPASAQPPP
PPPPAHSLQH YLSCGGSYPS MGHRASLACS PLGGGEPSPG AGEPSKGGPS GATAGAAGRA
TGPETAGGGA AGGGGGYRPI IQSPGYKTSK GGYGPATGGA TRPPPPRSTA TPKCQSLGGP
AAAYAAGKAS GAGGAGSQAY SPGQPQGLLG PQAYGQGFGG GQAQDLSKGP SYSGGPPQPP
SGPPPPGLAT CQSYSPDQLQ GQLYGVQSEP YPGPAAHSQG LPTASPSLSY STGHSPALSG
HGGGWGPSSL GGGGEASPSH IIRPLQSPPA TGRPPGVGSP GAPGKYLSSV LASAPFLAPP
GASSYAAGAG GYKGKGDGSE LLAGPGGSAA ERTEDEEFLI QHLLQAPSPP RTSGADGLVG
EDGPADAAKG LGGSGGAGGA PGTPYELAKE DPQRYHLQSV IRTSASLDEG ATAALELGLG
RMKDKKKGPE RGGETPEGLA TSVVHYGAGA KELGAFLQKS PPPPPPSAQA TQPAPHGLLL
EAGGPDLPMV LPPPPPQLLP SVLSHAPSPS PSAPKVGVHL LEPATRDGAP QPPPPPPPPM
PLQLEAHLRG HGLEPTAPSP RLRPEESLEP PGAMQELLGA LEPLPSGPGD PGVGPPNSEG
KDPAGAYRSP SPQGTKAPRF VPLTSICFPD SLLQDEERSF FPTMEEMFGG GAADDYGKAG
QTEDDGDPKT GAGPPPGPTA YDPYGPYCGG RASGTGPETP GLGLDHNKPP ELPSTVNAEP
LGLIQSGPHQ SAPPPPPPPP PPPPVSEPKG GLTSPIFCST KPKKLLKTSS FHLLRRRDPP
FQTPKKLYAQ EYEFEADEDK ADVPADIRLN PRRLPDLVSS CRSRPALSPL GDIDFCPPNP
GPDGPRRRGR KPTKAKRDGP PRPRGRPRIR PLEGPAMAGP ASITTDGAKK PRGRGRGRGR
KAEEMGGTRL EPLKPLKIKL SVPKAGEGLG APSNDVISGV DHNSLDSNLT REKIEAKIKE
VEEKQPEMKS GFMASFLDFL KSGKRHPPLY QAGLTPPLSP PKSVPASVPT RGLQPPPPTV
PTVPHPAPSG PFGLGGALEA AESEGLGLGC PSPCKRLDEE LKRNLETLPS FSSDEEDSVA
KNRDLQESIS SAISALDDPP LTGPKDTSTP EEPPLDTGPT ASGPPPLPSL PSSNSSGTPE
PPLLEEKPPP TPPPAPTPQP APPPPPPPPP VPALPSPTPL VTPVASSPPP PPPPPPPPPA
LPSPPPPPPP APTTVPPVAP PEEPPAPSPE DPEPPDARPL HLAKKQETAA VCGETDEEAG
ESGGEGIFRE RDEFVIRAED IPSLKLALQT GREPPPIWRV QKALLQKFTP EIKDGQRQFC
ATSNYLGYFG DAKNRYQRLY VKFLENVNKK DYVRVCARKP WHRPPLPVRR SGQTKGPTPV
GGNSAPPSKV QAPPPKPETP EKMTSEKPPE PAPEPAVPEP PAPEKPSPPR PVEKEKEKEK
EKEKEKERVT RPLRSERATS GRQMRTDRSL ATGQSTTSRL PKARPSKVKA EPPPKKRKKW
LKEAVGNASA GDGPGGSSSD SESSPGAPSE DERAVPGRLL KTRAMREMYR SYVEMLVSTA
LDPDMIQALE DTHDELYLPP MRKIDGLLNE HKKKVLKRLS LSPALQDALH TFPQLQVEQT
GEGSPEEGAV RLRPAGEPYN RKTLSKLKRS VVRAQEFKVE LEKSGYYTLY HSLHHYKYHT
FLRCRDQTLA IEGGAEDLGQ EEVVQQCMRN QPWLEQLFDS FSDLLAQAQA HSRCG
