ID   ATG32_LACTC             Reviewed;         472 AA.
AC   C5DH39;
DT   05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   25-MAY-2022, entry version 51.
DE   RecName: Full=Autophagy-related protein 32;
GN   Name=ATG32; OrderedLocusNames=KLTH0E01122g;
OS   Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)
OS   (Yeast) (Kluyveromyces thermotolerans).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Lachancea.
OX   NCBI_TaxID=559295;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 56472 / CBS 6340 / NRRL Y-8284;
RX   PubMed=19525356; DOI=10.1101/gr.091546.109;
RG   The Genolevures Consortium;
RA   Souciet J.-L., Dujon B., Gaillardin C., Johnston M., Baret P.V.,
RA   Cliften P., Sherman D.J., Weissenbach J., Westhof E., Wincker P., Jubin C.,
RA   Poulain J., Barbe V., Segurens B., Artiguenave F., Anthouard V.,
RA   Vacherie B., Val M.-E., Fulton R.S., Minx P., Wilson R., Durrens P.,
RA   Jean G., Marck C., Martin T., Nikolski M., Rolland T., Seret M.-L.,
RA   Casaregola S., Despons L., Fairhead C., Fischer G., Lafontaine I., Leh V.,
RA   Lemaire M., de Montigny J., Neuveglise C., Thierry A., Blanc-Lenfle I.,
RA   Bleykasten C., Diffels J., Fritsch E., Frangeul L., Goeffon A.,
RA   Jauniaux N., Kachouri-Lafond R., Payen C., Potier S., Pribylova L.,
RA   Ozanne C., Richard G.-F., Sacerdot C., Straub M.-L., Talla E.;
RT   "Comparative genomics of protoploid Saccharomycetaceae.";
RL   Genome Res. 19:1696-1709(2009).
CC   -!- FUNCTION: Mitophagy-specific receptor that recruits the autophagic
CC       machinery to mitochondria and regulates selective degradation of
CC       mitochondria. Mitophagy contributes to regulate mitochondrial quantity
CC       and quality by eliminating the mitochondria to a basal level to fulfill
CC       cellular energy requirements and preventing excess ROS production.
CC       Recruits ATG11 to the surface of mitochondria. Promotes also autophagy-
CC       dependent peroxisome degradation (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}. Vacuole membrane
CC       {ECO:0000250}; Single-pass membrane protein {ECO:0000250}.
CC       Preautophagosomal structure membrane {ECO:0000250}; Single-pass
CC       membrane protein {ECO:0000250}. Note=Is recruited to the
CC       preautophagosomal structure during mitophagy and imported into the
CC       vacuole along with mitochondria during starvation. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ATG32 family. {ECO:0000305}.
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DR   EMBL; CU928169; CAR23100.1; -; Genomic_DNA.
DR   RefSeq; XP_002553537.1; XM_002553491.1.
DR   AlphaFoldDB; C5DH39; -.
DR   SMR; C5DH39; -.
DR   STRING; 559295.C5DH39; -.
DR   EnsemblFungi; CAR23100; CAR23100; KLTH0E01122g.
DR   GeneID; 8291675; -.
DR   KEGG; lth:KLTH0E01122g; -.
DR   eggNOG; ENOG502QY5V; Eukaryota.
DR   HOGENOM; CLU_039418_0_0_1; -.
DR   InParanoid; C5DH39; -.
DR   OMA; IPICQPG; -.
DR   OrthoDB; 1273395at2759; -.
DR   Proteomes; UP000002036; Chromosome E.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Autophagy; Membrane; Mitochondrion; Mitochondrion outer membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Vacuole.
FT   CHAIN           1..472
FT                   /note="Autophagy-related protein 32"
FT                   /id="PRO_0000399759"
FT   TRANSMEM        356..372
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..35
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          58..80
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          113..139
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   472 AA;  53040 MW;  C93C86C05E9C0733 CRC64;
     MSQYITNPRQ QSRQLRRNSP SGQEPHFATS SVPLNQRNSI LDPHLSVLQL LDRADPPSEL
     SSLKHGEIAK PATPRRSGFE SVNCSISESW QSIKHTDCSM VNTQGDATHQ HAGILSSSDT
     SEDEPDAQLS PSPNNFAFPN SATSIFPEAP HNLEASSLRE YQNSEIANPR EENDNETVTM
     SLMNSSNSFV MPKLSLIQQS QKFCILIVGK PAQRFYRDIP RAYHKMFEVR DVGHLSPREM
     NKYSAVMVIF GEPKEGKELL EKVAAHNSNI IAVCQRGQQQ QISNILNRYS KSNEIRLVYH
     LTVMSDHQDV HRLLRYLNTL STEVDSGYET EVGSRKIRKR RKSSKKRSPQ ITVNRWVIWS
     ISLTVGVGLG YCISCLLSST SSTLSVTLRS GDEVTIMEDI HNSPHESPFD NYLRHLLLAV
     KRAVKQVNSS FKQYLSGQSL PVLWMQRIGK EWLSEASDPT LPGVTALDLV LV