ID   PRR1_TOBAC              Reviewed;         226 AA.
AC   P13046;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Pathogenesis-related protein R major form;
DE   AltName: Full=Thaumatin-like protein E22;
DE   Flags: Precursor;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=cv. Xanthi; TISSUE=Leaf;
RX   AGRICOLA=IND92000073; DOI=10.1007/BF00015674;
RA   Payne G., Middlesteadt W., Williams S., Desai N., Parks T.D., Dincher S.,
RA   Carnes M., Ryals J.;
RT   "Isolation and nucleotide sequence of a novel cDNA clone encoding the major
RT   form of pathogenesis-related protein R.";
RL   Plant Mol. Biol. 11:223-224(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=cv. Samsun NN;
RA   van Kan J.A.L., van de Rhee M.D., Zuidema D., Cornelissen B.J.C., Bol J.F.;
RT   "Structure of tobacco genes encoding thaumatin-like proteins.";
RL   Plant Mol. Biol. 12:153-155(1989).
RN   [3]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Leaf;
RA   Pierpoint W.S., Tatham A.S., Pappin D.J.C.;
RT   "Identification of the virus-induced protein of tobacco leaves that
RT   resembles the sweet-protein thaumatin.";
RL   Physiol. Mol. Plant Pathol. 31:291-298(1987).
CC   -!- SUBCELLULAR LOCATION: Vacuole.
CC   -!- MISCELLANEOUS: PR proteins are acid-soluble, protease-resistant
CC       proteins which accumulate in the intercellular spaces of many plants as
CC       a result of the hypersensitive reaction to a pathogen.
CC   -!- MISCELLANEOUS: PR-R exists as two isoforms in tobacco, a major and a
CC       minor form.
CC   -!- SIMILARITY: Belongs to the thaumatin family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00699}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X12739; CAA31235.1; -; mRNA.
DR   EMBL; X15224; CAA33293.1; -; Genomic_DNA.
DR   PIR; JH0230; JH0230.
DR   RefSeq; NP_001312145.1; NM_001325216.1.
DR   AlphaFoldDB; P13046; -.
DR   SMR; P13046; -.
DR   GeneID; 107776518; -.
DR   KEGG; nta:107776518; -.
DR   OMA; TTHARIW; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR   GO; GO:0006952; P:defense response; IBA:GO_Central.
DR   GO; GO:0009607; P:response to biotic stimulus; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.110.10; -; 1.
DR   InterPro; IPR037176; Osmotin/thaumatin-like_sf.
DR   InterPro; IPR001938; Thaumatin.
DR   InterPro; IPR017949; Thaumatin_CS.
DR   PANTHER; PTHR31048; PTHR31048; 1.
DR   Pfam; PF00314; Thaumatin; 1.
DR   PIRSF; PIRSF002703; Thaumatin; 1.
DR   PRINTS; PR00347; THAUMATIN.
DR   SMART; SM00205; THN; 1.
DR   SUPFAM; SSF49870; SSF49870; 1.
DR   PROSITE; PS00316; THAUMATIN_1; 1.
DR   PROSITE; PS51367; THAUMATIN_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Pathogenesis-related protein;
KW   Plant defense; Reference proteome; Signal; Vacuole.
FT   SIGNAL          1..25
FT   CHAIN           26..226
FT                   /note="Pathogenesis-related protein R major form"
FT                   /id="PRO_0000034028"
FT   DISULFID        34..225
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT   DISULFID        75..85
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT   DISULFID        90..96
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT   DISULFID        140..214
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT   DISULFID        145..197
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT   DISULFID        153..163
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT   DISULFID        167..176
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT   DISULFID        177..184
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT   CONFLICT        26
FT                   /note="A -> D (in Ref. 2)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   226 AA;  24667 MW;  20A187D043193C6D CRC64;
     MNFLKSFPFF AFLYFGQYFV AVTHAATFDI VNKCTYTVWA AASPGGGRRL DSGQSWSINV
     NPGTVQARIW GRTNCNFDGS GRGNCETGDC NGMLECQGYG KAPNTLAEFA LNQPNQDFVD
     ISLVDGFNIP MEFSPTNGGC RNLRCTAPIN EQCPAQLKTQ GGCNNPCTVI KTNEYCCTNG
     PGSCGPTDLS RFFKERCPDA YSYPQDDPTS LFTCPSGTNY RVVFCP