ID   PRRA_MYCBO              Reviewed;         233 AA.
AC   P0A5Z7; A0A1R3XX52; Q10531; X2BGG9;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Transcriptional regulatory protein PrrA;
GN   Name=prrA; OrderedLocusNames=BQ2027_MB0927C;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- FUNCTION: Member of the two-component regulatory system PrrB/PrrA that
CC       is involved specifically in early intracellular multiplication of
CC       Mycobacterium and is essential for its viability. Upon phosphorylation
CC       by PrrB, functions as a transcription regulator by direct binding to
CC       promoter regions of target genes to positively regulate their
CC       expression. Autoregulates its own expression.
CC       {ECO:0000250|UniProtKB:P9WGM1}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated by PrrB at Asp-58. {ECO:0000250|UniProtKB:P9WGM1}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=SIT99525.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; LT708304; SIT99525.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; NP_854584.1; NC_002945.3.
DR   AlphaFoldDB; P0A5Z7; -.
DR   SMR; P0A5Z7; -.
DR   EnsemblBacteria; SIT99525; SIT99525; BQ2027_MB0927C.
DR   PATRIC; fig|233413.5.peg.1008; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00383; trans_reg_C; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR039420; WalR-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR48111; PTHR48111; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM00448; REC; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Phosphoprotein; Transcription;
KW   Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..233
FT                   /note="Transcriptional regulatory protein PrrA"
FT                   /id="PRO_0000081328"
FT   DOMAIN          9..123
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        134..232
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT   MOD_RES         58
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGM1,
FT                   ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   233 AA;  25009 MW;  B8B38C20BF917082 CRC64;
     MDTGVTSPRV LVVDDDSDVL ASLERGLRLS GFEVATAVDG AEALRSATEN RPDAIVLDIN
     MPVLDGVSVV TALRAMDNDV PVCVLSARSS VDDRVAGLEA GADDYLVKPF VLAELVARVK
     ALLRRRGSTA TSSSETITVG PLEVDIPGRR ARVNGVDVDL TKREFDLLAV LAEHKTAVLS
     RAQLLELVWG YDFAADTNVV DVFIGYLRRK LEAGGGPRLL HTVRGVGFVL RMQ