ID   PRRA_MYCTO              Reviewed;         233 AA.
AC   P9WGM0; L0T590; P0A5Z6; Q10531;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 33.
DE   RecName: Full=Transcriptional regulatory protein PrrA;
GN   Name=prrA; OrderedLocusNames=MT0926;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Member of the two-component regulatory system PrrB/PrrA that
CC       is involved specifically in early intracellular multiplication of
CC       Mycobacterium and is essential for its viability. Upon phosphorylation
CC       by PrrB, functions as a transcription regulator by direct binding to
CC       promoter regions of target genes to positively regulate their
CC       expression. Autoregulates its own expression.
CC       {ECO:0000250|UniProtKB:P9WGM1}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated by PrrB at Asp-58. Also phosphorylated on Thr-6 by
CC       PknG/PknK/PknJ. The two phosphorylations act synergistically to
CC       activate the DNA-binding activity of PrrA.
CC       {ECO:0000250|UniProtKB:P9WGM1}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK45173.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE000516; AAK45173.1; ALT_INIT; Genomic_DNA.
DR   PIR; D70783; D70783.
DR   AlphaFoldDB; P9WGM0; -.
DR   SMR; P9WGM0; -.
DR   EnsemblBacteria; AAK45173; AAK45173; MT0926.
DR   KEGG; mtc:MT0926; -.
DR   HOGENOM; CLU_000445_30_1_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00383; trans_reg_C; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR039420; WalR-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR48111; PTHR48111; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM00448; REC; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Phosphoprotein; Transcription;
KW   Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..233
FT                   /note="Transcriptional regulatory protein PrrA"
FT                   /id="PRO_0000428338"
FT   DOMAIN          9..123
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        134..232
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT   MOD_RES         58
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   233 AA;  25009 MW;  B8B38C20BF917082 CRC64;
     MDTGVTSPRV LVVDDDSDVL ASLERGLRLS GFEVATAVDG AEALRSATEN RPDAIVLDIN
     MPVLDGVSVV TALRAMDNDV PVCVLSARSS VDDRVAGLEA GADDYLVKPF VLAELVARVK
     ALLRRRGSTA TSSSETITVG PLEVDIPGRR ARVNGVDVDL TKREFDLLAV LAEHKTAVLS
     RAQLLELVWG YDFAADTNVV DVFIGYLRRK LEAGGGPRLL HTVRGVGFVL RMQ