ID   PRRC_ECOLX              Reviewed;         396 AA.
AC   P17223;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   03-AUG-2022, entry version 43.
DE   RecName: Full=Anticodon nuclease {ECO:0000303|PubMed:1691706};
DE            Short=ACNase {ECO:0000303|PubMed:1691706};
DE            EC=3.1.-.- {ECO:0000269|PubMed:1691706};
DE   AltName: Full=Phage-less ACNase {ECO:0000303|PubMed:1691706};
GN   Name=prrC {ECO:0000303|PubMed:1691706};
GN   Synonyms=orfC {ECO:0000303|PubMed:1691706};
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=CTR5X;
RX   PubMed=1691706; DOI=10.1002/j.1460-2075.1990.tb08253.x;
RA   Levitz R., Chapman D., Amitsur M., Green R., Snyder L., Kaufmann G.;
RT   "The optional E. coli prr locus encodes a latent form of phage T4-induced
RT   anticodon nuclease.";
RL   EMBO J. 9:1383-1389(1990).
CC   -!- FUNCTION: Anticodon endonuclease (ACNase) that triggers the cleavage
CC       ligation of tRNA(Lys). It is activated by T4 stp protein and masked by
CC       the prrD protein (the endonuclease subunit of EcoprrI)
CC       (PubMed:1691706). The prr locus restricts phage T4 mutants lacking
CC       polynucleotide kinase or RNA ligase; T4 mutants lacking these genes
CC       manifest a T4-induced anticodon nuclease (ACNase). It is thought that
CC       Stp and other T4-encoded ACNase factors counteract the masking agents,
CC       thus activating the latent ACNase (Probable).
CC       {ECO:0000269|PubMed:1691706, ECO:0000305|PubMed:1691706}.
CC   -!- DISRUPTION PHENOTYPE: Loss of ACNase activity.
CC       {ECO:0000269|PubMed:1691706}.
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DR   EMBL; X52284; CAA36527.1; -; Genomic_DNA.
DR   PIR; S09627; S09627.
DR   RefSeq; WP_032251799.1; NZ_NWAV01000016.1.
DR   AlphaFoldDB; P17223; -.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   4: Predicted;
KW   Endonuclease; Hydrolase; Nuclease.
FT   CHAIN           1..396
FT                   /note="Anticodon nuclease"
FT                   /id="PRO_0000097050"
SQ   SEQUENCE   396 AA;  45568 MW;  607C9C9BC99C0894 CRC64;
     MGKTLSEIAQ QLSTPQKVKK TVHKEVEATR AVPKVQLIYA FNGTGKTRLS RDFKQLLESK
     VHDGEGEDEA EQSALSRKKI LYYNAFTEDL FYWDNDLQED AEPKLKVQPN SYTNWLLTLL
     KDLGQDSNIV RYFQRYANDK LTPHFNPDFT EITFSMERGN DERSAHIKLS KGEESNFIWS
     VFYTLLDQVV TILNVADPDA RETHAFDQLK YVFIDDPVSS LDDNHLIELA VNLAGLIKSS
     ESDLKFIITT HSPIFYNVLF NELNGKVCYM LESFEDGTFA LTEKYGDSNK SFSYHLHLKQ
     TIEQAIADNN VERYHFTLLR NLYEKTASFL GYPKWSELLP DDKQLYLSRI INFTSHSTLS
     NEAVAEPTPA EKATVKLLLD HLKNNCGFWQ QEQKNG