PRRT3_MOUSE
ID PRRT3_MOUSE Reviewed; 971 AA.
AC Q6PE13;
DT 03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Proline-rich transmembrane protein 3;
DE Flags: Precursor;
GN Name=Prrt3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain cortex;
RX PubMed=17114649; DOI=10.1074/mcp.m600046-mcp200;
RA Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F.,
RA Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B.,
RA Panse C., Schlapbach R., Mansuy I.M.;
RT "Qualitative and quantitative analyses of protein phosphorylation in naive
RT and stimulated mouse synaptosomal preparations.";
RL Mol. Cell. Proteomics 6:283-293(2007).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-768; SER-780; SER-783;
RP SER-789; SER-799; SER-845; SER-865; SER-894; SER-895 AND SER-903, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-771, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
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DR EMBL; BC058349; AAH58349.1; -; mRNA.
DR CCDS; CCDS20424.1; -.
DR RefSeq; NP_001276629.1; NM_001289700.1.
DR RefSeq; NP_766075.2; NM_172487.4.
DR RefSeq; XP_006505906.1; XM_006505843.3.
DR AlphaFoldDB; Q6PE13; -.
DR IntAct; Q6PE13; 1.
DR MINT; Q6PE13; -.
DR STRING; 10090.ENSMUSP00000098620; -.
DR GlyGen; Q6PE13; 2 sites.
DR iPTMnet; Q6PE13; -.
DR PhosphoSitePlus; Q6PE13; -.
DR SwissPalm; Q6PE13; -.
DR PaxDb; Q6PE13; -.
DR PeptideAtlas; Q6PE13; -.
DR PRIDE; Q6PE13; -.
DR ProteomicsDB; 291672; -.
DR Antibodypedia; 25871; 28 antibodies from 11 providers.
DR Ensembl; ENSMUST00000101059; ENSMUSP00000098620; ENSMUSG00000045009.
DR GeneID; 210673; -.
DR KEGG; mmu:210673; -.
DR UCSC; uc009dgp.2; mouse.
DR CTD; 285368; -.
DR MGI; MGI:2444810; Prrt3.
DR VEuPathDB; HostDB:ENSMUSG00000045009; -.
DR eggNOG; ENOG502QSMJ; Eukaryota.
DR GeneTree; ENSGT00730000111360; -.
DR HOGENOM; CLU_304190_0_0_1; -.
DR InParanoid; Q6PE13; -.
DR OrthoDB; 441294at2759; -.
DR PhylomeDB; Q6PE13; -.
DR TreeFam; TF333410; -.
DR BioGRID-ORCS; 210673; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Prrt3; mouse.
DR PRO; PR:Q6PE13; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q6PE13; protein.
DR Bgee; ENSMUSG00000045009; Expressed in lateral geniculate body and 94 other tissues.
DR ExpressionAtlas; Q6PE13; baseline and differential.
DR Genevisible; Q6PE13; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR043242; PRRT3.
DR PANTHER; PTHR47400; PTHR47400; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Membrane; Methylation; Phosphoprotein; Reference proteome;
KW Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..971
FT /note="Proline-rich transmembrane protein 3"
FT /id="PRO_0000251979"
FT TOPO_DOM 28..467
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 468..488
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 489..491
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 492..512
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 513..532
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 533..553
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 554..560
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 561..581
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 582..588
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 589..609
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 610..628
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 629..649
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 650..669
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 670..690
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 691..971
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 60..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 114..137
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 236..256
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 272..445
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 753..799
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 832..855
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 927..971
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 293..307
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 359..373
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 929..943
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 952..971
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 768
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 771
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 780
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 783
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 789
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 799
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 806
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5FWE3"
FT MOD_RES 845
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 865
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 894
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 895
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 903
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CARBOHYD 58
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 369
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 971 AA; 101224 MW; 3ED4E0DB57A01590 CRC64;
MAPSPQACTS PLLLLLLPCL GAGPALGRGL PRPLENSEPH MIPSESQTFD LFWEKLRNES
SWHSGDPQAR AEGPKKPADP YLGPALHGPK AAPGVQGERL LRADDLQLAR AFTSQGWTGP
PDSQELLEPE APEPHPVRAP RLTLVTTTPS SLLSAAILST ASQKPGGTAG QQPARNEELI
MVKAETHITQ ASPWDFQGSS HTPVPETDAV RTLVLGKQGG HEQGFQEAVQ GPLLTQQDPV
VPGVGSTPPV KVESTPEPGA QLDLALVRSL PLPEGLPAEP PKTGAGDTWE VSSLGPQPEQ
TDLLGGQDSP APQPIPPSAS DTSDGHLRPV SSLNGADPIS PQRVRGAMEA PGTPKSFIPD
LPNSAQAANG TESPVRALQP DEAEDWPGRP QSHPPAPPVQ APSTSRRGLV RVTTQRALGQ
PLPPEPSASS IVPIPASSPP ANATAPPLRW GPLRRVLSFS WELHVYGVGV LFLLPALLAL
VTVAAALAGP RLALVAAALV LVASGLRSAY MLTDPYGSQA RLGVRAGLVL YNLPFPLLLT
ALAALTLLGL GAGLPQPLQK PLLLGIVAPV HGTCLLATDL FSTSPVLNLL TQGLSCAWGA
SVALGTLCLC RRRLLEGPRG WDASPGPRLL AVAGSLGLLA SGLQLAASLW LYPGPGREGR
FSWAWWGVHF WLRLLELTWA LALALAALAA TRPRPPTEHA CWAKLLRLAC PAPTGKSEVP
ERPNNCYAGP SGLGTGGLDI SKSLIRNAAG EAGLPVTPGS GPWGSAASLG RGRPGGQRMS
RGSVGPAPSL SELDLRPPSP INLSRSIDAA LFREHLVRES VFQRCGLRGL ASSPTGGALR
PRRGSQPDAE LDGAGTSLLR GRCRSLTEVC LRTSLPQHVM EPPVGAAAAG TSGSSLDSFS
KGSLKISWNP WRHGLSSVDS LPLDELPSTV QLLPPPTPVP APARAGEPQG EGQSRCKSSE
SHSASSDTIE L
