PRRX1_MOUSE
ID PRRX1_MOUSE Reviewed; 245 AA.
AC P63013; P43271; Q02810; Q569N6;
DT 31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 31-AUG-2004, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Paired mesoderm homeobox protein 1;
DE AltName: Full=Homeobox protein K-2;
DE AltName: Full=Homeobox protein mHox;
DE AltName: Full=Paired-related homeobox protein 1;
DE Short=PRX-1;
GN Name=Prrx1; Synonyms=Pmx, Pmx1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RX PubMed=1360403; DOI=10.1242/dev.115.4.1087;
RA Cserjesi P., Lilly B., Bryson L., Wang Y., Sassoon D.A., Olson E.N.;
RT "MHox: a mesodermally restricted homeodomain protein that binds an
RT essential site in the muscle creatine kinase enhancer.";
RL Development 115:1087-1101(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RX PubMed=7910581; DOI=10.1006/geno.1994.1066;
RA Kern M.J., Argao E.A., Birkenmeier E.H., Rowe L.B., Potter S.S.;
RT "Genomic organization and chromosome localization of the murine homeobox
RT gene Pmx.";
RL Genomics 19:334-340(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=CD-1;
RX PubMed=1383943; DOI=10.1093/nar/20.19.5189;
RA Kern M.J., Witte D.P., Valerius M.T., Aronow B.J., Potter S.S.;
RT "A novel murine homeobox gene isolated by a tissue specific PCR cloning
RT strategy.";
RL Nucleic Acids Res. 20:5189-5195(1992).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-160, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic fibroblast;
RX PubMed=23806337; DOI=10.1016/j.molcel.2013.06.001;
RA Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y.,
RA Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.;
RT "SIRT5-mediated lysine desuccinylation impacts diverse metabolic
RT pathways.";
RL Mol. Cell 50:919-930(2013).
CC -!- FUNCTION: Acts as a transcriptional regulator of muscle creatine kinase
CC (MCK) and so has a role in the establishment of diverse mesodermal
CC muscle types. The protein binds to an A/T-rich element in the muscle
CC creatine enhancer.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=K2-a, PMX1-B, Prx1a;
CC IsoId=P63013-1, P43271-1;
CC Sequence=Displayed;
CC Name=2; Synonyms=K2-b, PMX1-A, Prx1b;
CC IsoId=P63013-2, P43271-2;
CC Sequence=VSP_002279;
CC -!- TISSUE SPECIFICITY: Expressed exclusively in mesodermally derived cell
CC types. During embryogenesis, highest levels of expression are found in
CC the mesenchyme and precartilage elements of the face and hind limbs. In
CC the adult, expression is restricted to skeletal muscle, heart and
CC uterus.
CC -!- DEVELOPMENTAL STAGE: Expressed during cardiogenesis.
CC -!- SIMILARITY: Belongs to the paired homeobox family. {ECO:0000305}.
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DR EMBL; L06502; AAA39672.1; -; mRNA.
DR EMBL; U03873; AAC52139.1; -; mRNA.
DR EMBL; X59725; CAA42410.1; -; mRNA.
DR EMBL; BC092372; AAH92372.1; -; mRNA.
DR CCDS; CCDS15427.1; -.
DR CCDS; CCDS15428.1; -. [P63013-2]
DR PIR; I48902; I48902.
DR PIR; S26076; S26076.
DR RefSeq; NP_035257.1; NM_011127.2. [P63013-1]
DR RefSeq; NP_783617.2; NM_175686.3. [P63013-2]
DR RefSeq; XP_006496766.1; XM_006496703.3. [P63013-2]
DR AlphaFoldDB; P63013; -.
DR SMR; P63013; -.
DR BioGRID; 202269; 2.
DR IntAct; P63013; 2.
DR STRING; 10090.ENSMUSP00000027878; -.
DR iPTMnet; P63013; -.
DR PhosphoSitePlus; P63013; -.
DR jPOST; P63013; -.
DR MaxQB; P63013; -.
DR PaxDb; P63013; -.
DR PRIDE; P63013; -.
DR ProteomicsDB; 291820; -.
DR ProteomicsDB; 291821; -. [P63013-2]
DR Antibodypedia; 34372; 436 antibodies from 31 providers.
DR DNASU; 18933; -.
DR Ensembl; ENSMUST00000027878; ENSMUSP00000027878; ENSMUSG00000026586. [P63013-1]
DR Ensembl; ENSMUST00000075805; ENSMUSP00000075203; ENSMUSG00000026586. [P63013-2]
DR GeneID; 18933; -.
DR KEGG; mmu:18933; -.
DR UCSC; uc007dhh.1; mouse. [P63013-2]
DR UCSC; uc007dhi.1; mouse.
DR CTD; 5396; -.
DR MGI; MGI:97712; Prrx1.
DR VEuPathDB; HostDB:ENSMUSG00000026586; -.
DR eggNOG; KOG0490; Eukaryota.
DR GeneTree; ENSGT00940000159466; -.
DR InParanoid; P63013; -.
DR OMA; YAHIMER; -.
DR OrthoDB; 1413050at2759; -.
DR PhylomeDB; P63013; -.
DR TreeFam; TF351612; -.
DR BioGRID-ORCS; 18933; 5 hits in 74 CRISPR screens.
DR ChiTaRS; Prrx1; mouse.
DR PRO; PR:P63013; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; P63013; protein.
DR Bgee; ENSMUSG00000026586; Expressed in secondary palatal shelf and 315 other tissues.
DR ExpressionAtlas; P63013; baseline and differential.
DR Genevisible; P63013; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0003677; F:DNA binding; IDA:MGI.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0071837; F:HMG box domain binding; IPI:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; IDA:MGI.
DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; ISO:MGI.
DR GO; GO:0048844; P:artery morphogenesis; IMP:MGI.
DR GO; GO:0051216; P:cartilage development; IMP:MGI.
DR GO; GO:0048701; P:embryonic cranial skeleton morphogenesis; IMP:MGI.
DR GO; GO:0030326; P:embryonic limb morphogenesis; IGI:MGI.
DR GO; GO:0048704; P:embryonic skeletal system morphogenesis; IMP:MGI.
DR GO; GO:0042472; P:inner ear morphogenesis; IGI:MGI.
DR GO; GO:0010463; P:mesenchymal cell proliferation; IGI:MGI.
DR GO; GO:0042474; P:middle ear morphogenesis; IMP:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR GO; GO:0048664; P:neuron fate determination; IDA:MGI.
DR GO; GO:0097150; P:neuronal stem cell population maintenance; IDA:MGI.
DR GO; GO:0100026; P:positive regulation of DNA repair by transcription from RNA polymerase II promoter; ISO:MGI.
DR GO; GO:0002053; P:positive regulation of mesenchymal cell proliferation; IGI:MGI.
DR GO; GO:0045880; P:positive regulation of smoothened signaling pathway; IGI:MGI.
DR GO; GO:2000648; P:positive regulation of stem cell proliferation; IGI:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0070570; P:regulation of neuron projection regeneration; IMP:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0060021; P:roof of mouth development; IMP:MGI.
DR GO; GO:0007224; P:smoothened signaling pathway; IGI:MGI.
DR GO; GO:0072089; P:stem cell proliferation; IGI:MGI.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR003654; OAR_dom.
DR InterPro; IPR043378; PRRX1/2.
DR PANTHER; PTHR46385; PTHR46385; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF03826; OAR; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS50803; OAR; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Developmental protein; DNA-binding;
KW Homeobox; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..245
FT /note="Paired mesoderm homeobox protein 1"
FT /id="PRO_0000049252"
FT DNA_BIND 94..153
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 54..103
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 222..235
FT /note="OAR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00138"
FT COMPBIAS 67..81
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 21
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P63014"
FT MOD_RES 160
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:23806337"
FT MOD_RES 197
FT /note="Phosphoserine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 200..245
FT /note="SAMATYSATCANNSPAQGINMANSIANLRLKAKEYSLQRNQVPTVN -> RS
FT SSLPRCCLHEGLHNGF (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:1360403,
FT ECO:0000303|PubMed:15489334, ECO:0000303|PubMed:7910581"
FT /id="VSP_002279"
SQ SEQUENCE 245 AA; 27269 MW; FF867F0D856E1115 CRC64;
MTSSYGHVLE RQPALGGRLD SPGNLDTLQA KKNFSVSHLL DLEEAGDMVA AQADESVGEA
GRSLLESPGL TSGSDTPQQD NDQLNSEEKK KRKQRRNRTT FNSSQLQALE RVFERTHYPD
AFVREDLARR VNLTEARVQV WFQNRRAKFR RNERAMLANK NASLLKSYSG DVTAVEQPIV
PRPAPRPTDY LSWGTASPYS AMATYSATCA NNSPAQGINM ANSIANLRLK AKEYSLQRNQ
VPTVN
