PRS23_HUMAN
ID PRS23_HUMAN Reviewed; 383 AA.
AC O95084; B2RDJ1; B4E2J3; Q6IBI0;
DT 16-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 181.
DE RecName: Full=Serine protease 23;
DE EC=3.4.21.-;
DE AltName: Full=Putative secreted protein Zsig13;
DE Flags: Precursor;
GN Name=PRSS23; Synonyms=ZSIG13; ORFNames=UNQ270/PRO307;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Umbilical vein;
RA Li X., Tedder T.F.;
RT "A novel serine protease from human umbilical vein endothelial cells.";
RL Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Sheppard P., Blumberg H., Jelinek L., Foster D., O'Hara P.;
RL Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Uterus;
RX PubMed=11230166; DOI=10.1101/gr.gr1547r;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J.,
RA Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W.,
RA Ottenwaelder B., Obermaier B., Tampe J., Heubner D., Wambutt R., Korn B.,
RA Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and analysis of
RT 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Placenta, and Trachea;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16554811; DOI=10.1038/nature04632;
RA Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T.,
RA Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G.,
RA Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C.,
RA Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A.,
RA Hattori M., Rogers J., Lander E.S., Sakaki Y.;
RT "Human chromosome 11 DNA sequence and analysis including novel gene
RT identification.";
RL Nature 440:497-500(2006).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Cervix;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [10]
RP PHOSPHORYLATION AT SER-109.
RX PubMed=26091039; DOI=10.1016/j.cell.2015.05.028;
RA Tagliabracci V.S., Wiley S.E., Guo X., Kinch L.N., Durrant E., Wen J.,
RA Xiao J., Cui J., Nguyen K.B., Engel J.L., Coon J.J., Grishin N.,
RA Pinna L.A., Pagliarini D.J., Dixon J.E.;
RT "A single kinase generates the majority of the secreted phosphoproteome.";
RL Cell 161:1619-1632(2015).
CC -!- INTERACTION:
CC O95084; Q9BY50: SEC11C; NbExp=3; IntAct=EBI-712503, EBI-2855401;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O95084-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O95084-2; Sequence=VSP_056628;
CC -!- SIMILARITY: Belongs to the peptidase S1 family. {ECO:0000305}.
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DR EMBL; AF015287; AAD01553.1; -; mRNA.
DR EMBL; AF193611; AAF07186.1; -; mRNA.
DR EMBL; AL136914; CAB66848.1; -; mRNA.
DR EMBL; AY359033; AAQ89392.1; -; mRNA.
DR EMBL; CR456824; CAG33105.1; -; mRNA.
DR EMBL; AK304301; BAG65155.1; -; mRNA.
DR EMBL; AK315562; BAG37938.1; -; mRNA.
DR EMBL; AP000654; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AP001528; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471076; EAW75145.1; -; Genomic_DNA.
DR EMBL; BC001278; AAH01278.1; -; mRNA.
DR CCDS; CCDS8278.1; -. [O95084-1]
DR RefSeq; NP_001280107.1; NM_001293178.1.
DR RefSeq; NP_001280108.1; NM_001293179.1. [O95084-1]
DR RefSeq; NP_001280109.1; NM_001293180.1. [O95084-1]
DR RefSeq; NP_009104.2; NM_007173.5. [O95084-1]
DR AlphaFoldDB; O95084; -.
DR BioGRID; 116279; 56.
DR IntAct; O95084; 44.
DR STRING; 9606.ENSP00000280258; -.
DR MEROPS; S01.309; -.
DR GlyConnect; 1736; 2 N-Linked glycans (2 sites).
DR GlyGen; O95084; 2 sites, 2 N-linked glycans (2 sites).
DR iPTMnet; O95084; -.
DR PhosphoSitePlus; O95084; -.
DR BioMuta; PRSS23; -.
DR EPD; O95084; -.
DR jPOST; O95084; -.
DR MassIVE; O95084; -.
DR MaxQB; O95084; -.
DR PaxDb; O95084; -.
DR PeptideAtlas; O95084; -.
DR PRIDE; O95084; -.
DR ProteomicsDB; 50649; -. [O95084-1]
DR ProteomicsDB; 5825; -.
DR TopDownProteomics; O95084-1; -. [O95084-1]
DR Antibodypedia; 31454; 79 antibodies from 20 providers.
DR DNASU; 11098; -.
DR Ensembl; ENST00000280258.6; ENSP00000280258.4; ENSG00000150687.12. [O95084-1]
DR GeneID; 11098; -.
DR KEGG; hsa:11098; -.
DR MANE-Select; ENST00000280258.6; ENSP00000280258.4; NM_007173.6; NP_009104.3.
DR UCSC; uc001pcb.4; human. [O95084-1]
DR CTD; 11098; -.
DR DisGeNET; 11098; -.
DR GeneCards; PRSS23; -.
DR HGNC; HGNC:14370; PRSS23.
DR HPA; ENSG00000150687; Tissue enhanced (cervix).
DR MalaCards; PRSS23; -.
DR MIM; 618376; gene.
DR neXtProt; NX_O95084; -.
DR OpenTargets; ENSG00000150687; -.
DR PharmGKB; PA134952846; -.
DR VEuPathDB; HostDB:ENSG00000150687; -.
DR eggNOG; ENOG502QTW6; Eukaryota.
DR GeneTree; ENSGT00390000000155; -.
DR HOGENOM; CLU_055829_0_0_1; -.
DR InParanoid; O95084; -.
DR OMA; RQQQKWE; -.
DR OrthoDB; 1245017at2759; -.
DR PhylomeDB; O95084; -.
DR TreeFam; TF329011; -.
DR PathwayCommons; O95084; -.
DR Reactome; R-HSA-381426; Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
DR Reactome; R-HSA-8957275; Post-translational protein phosphorylation.
DR SignaLink; O95084; -.
DR BioGRID-ORCS; 11098; 14 hits in 1078 CRISPR screens.
DR ChiTaRS; PRSS23; human.
DR GeneWiki; PRSS23; -.
DR GenomeRNAi; 11098; -.
DR Pharos; O95084; Tbio.
DR PRO; PR:O95084; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; O95084; protein.
DR Bgee; ENSG00000150687; Expressed in nasal cavity epithelium and 205 other tissues.
DR ExpressionAtlas; O95084; baseline and differential.
DR Genevisible; O95084; HS.
DR GO; GO:0005788; C:endoplasmic reticulum lumen; TAS:Reactome.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:LIFEdb.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 2.40.10.10; -; 2.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR001254; Trypsin_dom.
DR InterPro; IPR018114; TRYPSIN_HIS.
DR Pfam; PF00089; Trypsin; 1.
DR SUPFAM; SSF50494; SSF50494; 1.
DR PROSITE; PS00134; TRYPSIN_HIS; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Disulfide bond; Glycoprotein; Hydrolase;
KW Phosphoprotein; Protease; Reference proteome; Secreted; Serine protease;
KW Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..383
FT /note="Serine protease 23"
FT /id="PRO_0000027847"
FT ACT_SITE 175
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10078"
FT ACT_SITE 240
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10078"
FT ACT_SITE 316
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10078"
FT MOD_RES 109
FT /note="Phosphoserine; by FAM20C"
FT /evidence="ECO:0000269|PubMed:26091039"
FT CARBOHYD 93
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 207
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 160..176
FT /evidence="ECO:0000250"
FT VAR_SEQ 49..80
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_056628"
SQ SEQUENCE 383 AA; 43001 MW; 46EB6C11ABFD5E8F CRC64;
MAGIPGLLFL LFFLLCAVGQ VSPYSAPWKP TWPAYRLPVV LPQSTLNLAK PDFGAEAKLE
VSSSCGPQCH KGTPLPTYEE AKQYLSYETL YANGSRTETQ VGIYILSSSG DGAQHRDSGS
SGKSRRKRQI YGYDSRFSIF GKDFLLNYPF STSVKLSTGC TGTLVAEKHV LTAAHCIHDG
KTYVKGTQKL RVGFLKPKFK DGGRGANDST SAMPEQMKFQ WIRVKRTHVP KGWIKGNAND
IGMDYDYALL ELKKPHKRKF MKIGVSPPAK QLPGGRIHFS GYDNDRPGNL VYRFCDVKDE
TYDLLYQQCD AQPGASGSGV YVRMWKRQQQ KWERKIIGIF SGHQWVDMNG SPQDFNVAVR
ITPLKYAQIC YWIKGNYLDC REG
