PRS27_HUMAN
ID PRS27_HUMAN Reviewed; 290 AA.
AC Q9BQR3;
DT 18-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Serine protease 27;
DE EC=3.4.21.-;
DE AltName: Full=Marapsin;
DE AltName: Full=Pancreasin;
DE Flags: Precursor;
GN Name=PRSS27; Synonyms=MPN; ORFNames=UNQ1884/PRO4327;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Fortunato M., Dando P.M., Rawlings N.D., Barrett A.J.;
RT "Cloning, sequencing and expression of marapsin, a human serine
RT proteinase.";
RL Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Okaze H., Hayashi A., Kozuma S., Saito T.;
RL Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], GLYCOSYLATION, AND TISSUE SPECIFICITY.
RX PubMed=12441343; DOI=10.1074/jbc.m209353200;
RA Bhagwandin V.J., Hau L.W.-T., Mallen-St Clair J., Wolters P.J.,
RA Caughey G.H.;
RT "Structure and activity of human pancreasin, a novel tryptic serine
RT peptidase expressed primarily by the pancreas.";
RL J. Biol. Chem. 278:3363-3371(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Expressed predominantly in the pancreas.
CC {ECO:0000269|PubMed:12441343}.
CC -!- PTM: N-glycosylated. {ECO:0000269|PubMed:12441343}.
CC -!- SIMILARITY: Belongs to the peptidase S1 family. {ECO:0000255|PROSITE-
CC ProRule:PRU00274}.
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DR EMBL; AJ306593; CAC35467.1; -; mRNA.
DR EMBL; AB056161; BAB85497.1; -; mRNA.
DR EMBL; AY030095; AAK38168.1; -; mRNA.
DR EMBL; AY359106; AAQ89464.1; -; mRNA.
DR EMBL; BC034294; AAH34294.1; -; mRNA.
DR CCDS; CCDS10476.1; -.
DR RefSeq; NP_001305324.1; NM_001318395.1.
DR RefSeq; NP_114154.1; NM_031948.4.
DR AlphaFoldDB; Q9BQR3; -.
DR SMR; Q9BQR3; -.
DR BioGRID; 123797; 1.
DR STRING; 9606.ENSP00000306390; -.
DR MEROPS; S01.074; -.
DR GlyGen; Q9BQR3; 2 sites.
DR iPTMnet; Q9BQR3; -.
DR PhosphoSitePlus; Q9BQR3; -.
DR BioMuta; PRSS27; -.
DR DMDM; 18202734; -.
DR MassIVE; Q9BQR3; -.
DR PaxDb; Q9BQR3; -.
DR PeptideAtlas; Q9BQR3; -.
DR PRIDE; Q9BQR3; -.
DR ProteomicsDB; 78710; -.
DR Antibodypedia; 23859; 223 antibodies from 23 providers.
DR DNASU; 83886; -.
DR Ensembl; ENST00000302641.8; ENSP00000306390.3; ENSG00000172382.10.
DR GeneID; 83886; -.
DR KEGG; hsa:83886; -.
DR MANE-Select; ENST00000302641.8; ENSP00000306390.3; NM_031948.5; NP_114154.1.
DR UCSC; uc002crf.4; human.
DR CTD; 83886; -.
DR DisGeNET; 83886; -.
DR GeneCards; PRSS27; -.
DR HGNC; HGNC:15475; PRSS27.
DR HPA; ENSG00000172382; Group enriched (esophagus, vagina).
DR MIM; 608018; gene.
DR neXtProt; NX_Q9BQR3; -.
DR OpenTargets; ENSG00000172382; -.
DR PharmGKB; PA33837; -.
DR VEuPathDB; HostDB:ENSG00000172382; -.
DR eggNOG; KOG3627; Eukaryota.
DR GeneTree; ENSGT00940000162015; -.
DR HOGENOM; CLU_006842_0_4_1; -.
DR InParanoid; Q9BQR3; -.
DR OMA; YILPVCM; -.
DR OrthoDB; 1314811at2759; -.
DR PhylomeDB; Q9BQR3; -.
DR TreeFam; TF351676; -.
DR PathwayCommons; Q9BQR3; -.
DR SignaLink; Q9BQR3; -.
DR BioGRID-ORCS; 83886; 12 hits in 1066 CRISPR screens.
DR GenomeRNAi; 83886; -.
DR Pharos; Q9BQR3; Tbio.
DR PRO; PR:Q9BQR3; -.
DR Proteomes; UP000005640; Chromosome 16.
DR RNAct; Q9BQR3; protein.
DR Bgee; ENSG00000172382; Expressed in lower esophagus mucosa and 103 other tissues.
DR ExpressionAtlas; Q9BQR3; baseline and differential.
DR Genevisible; Q9BQR3; HS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:Ensembl.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd00190; Tryp_SPc; 1.
DR Gene3D; 2.40.10.10; -; 2.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR001314; Peptidase_S1A.
DR InterPro; IPR001254; Trypsin_dom.
DR InterPro; IPR018114; TRYPSIN_HIS.
DR InterPro; IPR033116; TRYPSIN_SER.
DR Pfam; PF00089; Trypsin; 1.
DR PRINTS; PR00722; CHYMOTRYPSIN.
DR SMART; SM00020; Tryp_SPc; 1.
DR SUPFAM; SSF50494; SSF50494; 1.
DR PROSITE; PS50240; TRYPSIN_DOM; 1.
DR PROSITE; PS00134; TRYPSIN_HIS; 1.
DR PROSITE; PS00135; TRYPSIN_SER; 1.
PE 1: Evidence at protein level;
KW Disulfide bond; Glycoprotein; Hydrolase; Protease; Reference proteome;
KW Secreted; Serine protease; Signal; Zymogen.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT PROPEP 23..34
FT /note="Activation peptide"
FT /evidence="ECO:0000255"
FT /id="PRO_0000027506"
FT CHAIN 35..290
FT /note="Serine protease 27"
FT /id="PRO_0000027507"
FT DOMAIN 35..277
FT /note="Peptidase S1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT ACT_SITE 75
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 124
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 229
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT CARBOHYD 55
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 79
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 60..76
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 158..235
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 191..214
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 225..253
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
SQ SEQUENCE 290 AA; 31940 MW; 67BDC93EC70BFF7B CRC64;
MRRPAAVPLL LLLCFGSQRA KAATACGRPR MLNRMVGGQD TQEGEWPWQV SIQRNGSHFC
GGSLIAEQWV LTAAHCFRNT SETSLYQVLL GARQLVQPGP HAMYARVRQV ESNPLYQGTA
SSADVALVEL EAPVPFTNYI LPVCLPDPSV IFETGMNCWV TGWGSPSEED LLPEPRILQK
LAVPIIDTPK CNLLYSKDTE FGYQPKTIKN DMLCAGFEEG KKDACKGDSG GPLVCLVGQS
WLQAGVISWG EGCARQNRPG VYIRVTAHHN WIHRIIPKLQ FQPARLGGQK
