PRS35_MOUSE
ID PRS35_MOUSE Reviewed; 409 AA.
AC Q8C0F9; Q8C0D6; Q8C0L5;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Inactive serine protease 35;
DE Flags: Precursor;
GN Name=Prss35;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND
RP INDUCTION.
RC STRAIN=C57BL/6J; TISSUE=Ovary;
RX PubMed=16870946; DOI=10.1095/biolreprod.106.052290;
RA Miyakoshi K., Murphy M.J., Yeoman R.R., Mitra S., Dubay C.J.,
RA Hennebold J.D.;
RT "The identification of novel ovarian proteases through the use of genomic
RT and bioinformatic methodologies.";
RL Biol. Reprod. 75:823-835(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Head, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: In ovary, it localizes to the theca cells of pre-
CC antral follicles, the theca and granulosa cells of pre-ovulatory and
CC ovulatory follicles, as well as to the developing corpus luteum.
CC {ECO:0000269|PubMed:16870946}.
CC -!- DEVELOPMENTAL STAGE: Increases around the time of ovulation and
CC remained elevated in the developing corpus luteum.
CC {ECO:0000269|PubMed:16870946}.
CC -!- INDUCTION: Expression is progesterone-dependent regulation prior to
CC follicle rupture. {ECO:0000269|PubMed:16870946}.
CC -!- SIMILARITY: Belongs to the peptidase S1 family. {ECO:0000305}.
CC -!- CAUTION: Although related to peptidase S1 family, lacks the conserved
CC active Ser residue in position 342 which is replaced by a Thr,
CC suggesting that it has no protease activity. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; DQ223037; ABB46197.1; -; mRNA.
DR EMBL; AK030671; BAC27073.1; -; mRNA.
DR EMBL; AK031411; BAC27392.1; -; mRNA.
DR EMBL; AK031644; BAC27491.1; -; mRNA.
DR EMBL; BC075675; AAH75675.1; -; mRNA.
DR CCDS; CCDS40714.1; -.
DR RefSeq; NP_848853.2; NM_178738.3.
DR RefSeq; XP_006511233.1; XM_006511170.3.
DR RefSeq; XP_006511234.1; XM_006511171.3.
DR RefSeq; XP_006511235.1; XM_006511172.3.
DR AlphaFoldDB; Q8C0F9; -.
DR STRING; 10090.ENSMUSP00000035271; -.
DR MEROPS; S01.994; -.
DR GlyGen; Q8C0F9; 1 site.
DR PhosphoSitePlus; Q8C0F9; -.
DR PaxDb; Q8C0F9; -.
DR PRIDE; Q8C0F9; -.
DR ProteomicsDB; 291902; -.
DR Antibodypedia; 31685; 82 antibodies from 19 providers.
DR DNASU; 244954; -.
DR Ensembl; ENSMUST00000036426; ENSMUSP00000035271; ENSMUSG00000033491.
DR Ensembl; ENSMUST00000179574; ENSMUSP00000137445; ENSMUSG00000033491.
DR GeneID; 244954; -.
DR KEGG; mmu:244954; -.
DR UCSC; uc012gxu.1; mouse.
DR CTD; 167681; -.
DR MGI; MGI:2444800; Prss35.
DR VEuPathDB; HostDB:ENSMUSG00000033491; -.
DR eggNOG; ENOG502QV0K; Eukaryota.
DR GeneTree; ENSGT00390000000155; -.
DR HOGENOM; CLU_055829_0_0_1; -.
DR InParanoid; Q8C0F9; -.
DR OMA; MEQDFMW; -.
DR OrthoDB; 1245017at2759; -.
DR PhylomeDB; Q8C0F9; -.
DR TreeFam; TF329011; -.
DR BioGRID-ORCS; 244954; 2 hits in 73 CRISPR screens.
DR PRO; PR:Q8C0F9; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q8C0F9; protein.
DR Bgee; ENSMUSG00000033491; Expressed in adrenal gland and 118 other tissues.
DR Genevisible; Q8C0F9; MM.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR Gene3D; 2.40.10.10; -; 4.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR001254; Trypsin_dom.
DR InterPro; IPR018114; TRYPSIN_HIS.
DR Pfam; PF00089; Trypsin; 1.
DR SUPFAM; SSF50494; SSF50494; 1.
DR PROSITE; PS00134; TRYPSIN_HIS; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Glycoprotein; Reference proteome; Secreted;
KW Serine protease homolog; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..409
FT /note="Inactive serine protease 35"
FT /id="PRO_0000299360"
FT DOMAIN 124..404
FT /note="Peptidase S1"
FT REGION 188..247
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 188..205
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 90
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 154..170
FT /evidence="ECO:0000250"
FT CONFLICT 169
FT /note="H -> N (in Ref. 2; BAC27491)"
FT /evidence="ECO:0000305"
FT CONFLICT 266
FT /note="G -> S (in Ref. 2; BAC27073)"
FT /evidence="ECO:0000305"
FT CONFLICT 302
FT /note="R -> Q (in Ref. 2; BAC27491)"
FT /evidence="ECO:0000305"
FT CONFLICT 383
FT /note="V -> E (in Ref. 2; BAC27073)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 409 AA; 45787 MW; 6E22D4A908E7EFE5 CRC64;
MENTLLWLVI LIPGWALSDG SETELDFTWH LSRIPQVVSE NTIHLASPTF QADAGVVKAT
VCGIECQEEL PAPSLSQLEE SLSYETIFEN GTRTLTRVKV QGLVLEPTRN SSVKGAHPRR
RRQVYGTDSR FSILDKRFAT NFPFNTAVKL STGCSGTLVS PNHVLTAAHC VHDGKDYVKG
SKKLRVGVLK MRNKGGRKKR RGSKRSRREA ESAGQSQAHL RESTTQRPGK KSRRGPRVTQ
GRPSFQWTRV KSTHIPKGWV RGENGGLALD YDYALLELKR AHKQQHMELG VSPTITKLPG
GRIHFSGFDN DRDEQLVYRF CSVSEESNDL LYQYCDAEAG STGSGIYLRL KEPGQKNWKR
KIVAVYSGHQ WVDVHGVQKD YNVAVRITPL KYAQICLWIH GNAANCAYG
