ID   PRS40_RAT               Reviewed;         369 AA.
AC   Q6AXZ6;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Serine protease 40;
DE            EC=3.4.21.-;
DE   AltName: Full=Testicular serine protease 2;
DE   Flags: Precursor;
GN   Name=Prss40; Synonyms=Tesp2;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: May play an important role in the sperm/egg interaction;
CC       released during the acrosome reaction. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, acrosome.
CC       Secreted {ECO:0000250}.
CC   -!- MISCELLANEOUS: There appears to be no human ortholog of this protein.
CC   -!- SIMILARITY: Belongs to the peptidase S1 family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00274}.
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DR   EMBL; BC079254; AAH79254.1; -; mRNA.
DR   RefSeq; NP_001013244.1; NM_001013226.1.
DR   AlphaFoldDB; Q6AXZ6; -.
DR   SMR; Q6AXZ6; -.
DR   STRING; 10116.ENSRNOP00000038053; -.
DR   MEROPS; S01.985; -.
DR   PaxDb; Q6AXZ6; -.
DR   Ensembl; ENSRNOT00000034214; ENSRNOP00000038053; ENSRNOG00000023858.
DR   GeneID; 363215; -.
DR   KEGG; rno:363215; -.
DR   UCSC; RGD:1561330; rat.
DR   CTD; 21755; -.
DR   RGD; 1561330; Prss40.
DR   eggNOG; KOG3627; Eukaryota.
DR   GeneTree; ENSGT00940000157345; -.
DR   InParanoid; Q6AXZ6; -.
DR   OrthoDB; 1314811at2759; -.
DR   PhylomeDB; Q6AXZ6; -.
DR   TreeFam; TF351692; -.
DR   PRO; PR:Q6AXZ6; -.
DR   Proteomes; UP000002494; Chromosome 9.
DR   Bgee; ENSRNOG00000023858; Expressed in testis and 12 other tissues.
DR   ExpressionAtlas; Q6AXZ6; baseline and differential.
DR   GO; GO:0001669; C:acrosomal vesicle; IEA:UniProtKB-SubCell.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central.
DR   GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR   CDD; cd00190; Tryp_SPc; 1.
DR   Gene3D; 2.40.10.10; -; 1.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR   InterPro; IPR001314; Peptidase_S1A.
DR   InterPro; IPR001254; Trypsin_dom.
DR   InterPro; IPR033116; TRYPSIN_SER.
DR   Pfam; PF00089; Trypsin; 1.
DR   PRINTS; PR00722; CHYMOTRYPSIN.
DR   SMART; SM00020; Tryp_SPc; 1.
DR   SUPFAM; SSF50494; SSF50494; 1.
DR   PROSITE; PS50240; TRYPSIN_DOM; 1.
DR   PROSITE; PS00135; TRYPSIN_SER; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasmic vesicle; Disulfide bond; Hydrolase; Protease;
KW   Reference proteome; Secreted; Serine protease; Signal.
FT   SIGNAL          1..33
FT                   /evidence="ECO:0000255"
FT   CHAIN           34..369
FT                   /note="Serine protease 40"
FT                   /id="PRO_0000344983"
FT   DOMAIN          70..314
FT                   /note="Peptidase S1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   ACT_SITE        110
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        160
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        265
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   DISULFID        95..111
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        194..271
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        227..250
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        261..289
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
SQ   SEQUENCE   369 AA;  41448 MW;  F96511B4F112B919 CRC64;
     MWGSRAQQSG PDRGRTCLLA VLLLSFSLFQ LHAQDYKPSQ TPPPTSDALL KPMGRVQKEI
     CGKTKFQGKI YGGSIAKAER WPWQASLIFR GRHICGAVLI DKNWVASAAH CFKRSLKPSD
     YRILLGYNEL SNPSNYSRQM TLSKVIVHED YNKLHSQEKD IVLIQLHLPV RYSSHIFPVC
     VPDQTTKEPS DESCWISGWG MVTDDKFLQA PFPLLDSEVF LMNDQECEAF FQTPQISITE
     YDAIKDDMIC AGDITNQKST CRGDSGGPLV CLLDSYWYLV GLASWSGACL EPIHSPSIFT
     RVSHFSDWIE KKKADTPDVD PSLAPLEETA PSLIGWRNYS AGTTLEPRIC TTLLSSQVLL
     LQSIWLRIL