PRS43_MOUSE
ID PRS43_MOUSE Reviewed; 382 AA.
AC Q76HL1;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Putative inactive serine protease 43 {ECO:0000305};
DE Flags: Precursor;
GN Name=Prss43 {ECO:0000312|MGI:MGI:2684822};
GN Synonyms=Tessp3 {ECO:0000303|PubMed:23536369};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000312|EMBL:BAD06396.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND TOPOLOGY.
RX PubMed=23536369; DOI=10.1095/biolreprod.112.106328;
RA Yoneda R., Takahashi T., Matsui H., Takano N., Hasebe Y., Ogiwara K.,
RA Kimura A.P.;
RT "Three testis-specific paralogous serine proteases play different roles in
RT murine spermatogenesis and are involved in germ cell survival during
RT meiosis.";
RL Biol. Reprod. 88:118-118(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- FUNCTION: Plays a role in spermatogenesis. Involved in germ cell
CC survival during meiosis. {ECO:0000269|PubMed:23536369}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:23536369};
CC Lipid-anchor, GPI-anchor {ECO:0000269|PubMed:23536369}.
CC -!- TISSUE SPECIFICITY: Testis-specific (PubMed:23536369). Expressed in
CC germ cells at the stages from late pachytene spermatocytes to
CC spermatids (PubMed:23536369). {ECO:0000269|PubMed:23536369}.
CC -!- DEVELOPMENTAL STAGE: In testis, expressed at all stages from the late
CC pachytene primary spermatocyte to the secondary spermatocyte. Not
CC detected at day 7 after birth. Expression is detected at day 14 and
CC increases dramatically at day 21 and reach a peak at day 28 to remain
CC high until day 56. {ECO:0000269|PubMed:23536369}.
CC -!- SIMILARITY: Belongs to the peptidase S1 family. {ECO:0000305}.
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DR EMBL; AC139378; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AB100999; BAD06396.1; -; mRNA.
DR CCDS; CCDS23574.1; -.
DR RefSeq; NP_955765.1; NM_199471.1.
DR AlphaFoldDB; Q76HL1; -.
DR SMR; Q76HL1; -.
DR STRING; 10090.ENSMUSP00000076752; -.
DR MEROPS; S01.099; -.
DR PaxDb; Q76HL1; -.
DR PRIDE; Q76HL1; -.
DR ProteomicsDB; 334438; -.
DR DNASU; 272643; -.
DR Ensembl; ENSMUST00000077549; ENSMUSP00000076752; ENSMUSG00000058398.
DR GeneID; 272643; -.
DR KEGG; mmu:272643; -.
DR UCSC; uc009ruv.1; mouse.
DR CTD; 272643; -.
DR MGI; MGI:2684822; Prss43.
DR VEuPathDB; HostDB:ENSMUSG00000058398; -.
DR eggNOG; KOG3627; Eukaryota.
DR GeneTree; ENSGT00940000162829; -.
DR HOGENOM; CLU_006842_0_4_1; -.
DR InParanoid; Q76HL1; -.
DR OMA; CGHRITE; -.
DR OrthoDB; 1314811at2759; -.
DR PhylomeDB; Q76HL1; -.
DR TreeFam; TF351676; -.
DR BioGRID-ORCS; 272643; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Prss43; mouse.
DR PRO; PR:Q76HL1; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q76HL1; protein.
DR Bgee; ENSMUSG00000058398; Expressed in spermatocyte and 12 other tissues.
DR GO; GO:0046658; C:anchored component of plasma membrane; IDA:MGI.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0007281; P:germ cell development; IDA:MGI.
DR GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR GO; GO:0007283; P:spermatogenesis; IDA:MGI.
DR CDD; cd00190; Tryp_SPc; 1.
DR Gene3D; 2.40.10.10; -; 2.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR001314; Peptidase_S1A.
DR InterPro; IPR001254; Trypsin_dom.
DR InterPro; IPR018114; TRYPSIN_HIS.
DR Pfam; PF00089; Trypsin; 1.
DR PRINTS; PR00722; CHYMOTRYPSIN.
DR SMART; SM00020; Tryp_SPc; 1.
DR SUPFAM; SSF50494; SSF50494; 1.
DR PROSITE; PS50240; TRYPSIN_DOM; 1.
DR PROSITE; PS00134; TRYPSIN_HIS; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Differentiation; Disulfide bond; Glycoprotein; GPI-anchor;
KW Hydrolase; Lipoprotein; Membrane; Protease; Reference proteome; Signal;
KW Spermatogenesis; Transmembrane; Transmembrane helix.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..382
FT /note="Putative inactive serine protease 43"
FT /evidence="ECO:0000255"
FT /id="PRO_5015098640"
FT TRANSMEM 362..382
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 119..355
FT /note="Peptidase S1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT REGION 30..97
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 34..53
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 56..72
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 81..97
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 159
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT ACT_SITE 205
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT ACT_SITE 307
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 144..160
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 239..313
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 272..293
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 303..331
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
SQ SEQUENCE 382 AA; 42203 MW; A11894C74E8EEB6E CRC64;
MGGFCGADRG GFLALLVWLQ LLQPLFSGTY KPREDSGVMH RPQRPRRPRS DPEAPAQQSR
LKSLSISHPS GVPVSVDRTE IPGSGSPSGT TTKITLENRR SSLGGPFFTD TCGHRITEVD
PGSLSAGRKW PWQVSLQSQN EHVCGGSLIS HRWVLTAAHC IYEQEEYMVM LGDDMLHSES
ESVTLVPVQD IIFPSNFDIQ TMRNDIALAL LYFPVNYSSL IQPVCLPEEP FRVKNGTVCW
VTGWGQQNEI DAGFASILLQ EVQQRILLQK HCNTLFQRQL GTSKNLVIKG MICGLQDSGQ
SLCWGDSGNP LVCESDNTWT QVGIMSWGIN CNGVPVLSVY TDIAEYNEWV SYVLSQASRM
DPMGVLVLYL SLVFPLALLV AL
