PRS46_MOUSE
ID PRS46_MOUSE Reviewed; 314 AA.
AC Q5M8S2; Q8C1Q8;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2012, sequence version 2.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Serine protease 46;
DE EC=3.4.21.-;
GN Name=Prss46;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5M8S2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5M8S2-2; Sequence=VSP_044025;
CC -!- SIMILARITY: Belongs to the peptidase S1 family. {ECO:0000255|PROSITE-
CC ProRule:PRU00274}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC25167.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK007173; BAC25167.1; ALT_FRAME; mRNA.
DR EMBL; AC139378; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC087878; AAH87878.1; -; mRNA.
DR CCDS; CCDS23576.2; -. [Q5M8S2-1]
DR RefSeq; NP_898926.2; NM_183103.2. [Q5M8S2-1]
DR AlphaFoldDB; Q5M8S2; -.
DR SMR; Q5M8S2; -.
DR STRING; 10090.ENSMUSP00000135787; -.
DR MEROPS; S01.250; -.
DR PaxDb; Q5M8S2; -.
DR PRIDE; Q5M8S2; -.
DR ProteomicsDB; 291907; -. [Q5M8S2-1]
DR ProteomicsDB; 291908; -. [Q5M8S2-2]
DR DNASU; 74306; -.
DR Ensembl; ENSMUST00000176403; ENSMUSP00000135787; ENSMUSG00000049719. [Q5M8S2-1]
DR GeneID; 74306; -.
DR KEGG; mmu:74306; -.
DR UCSC; uc009ruy.1; mouse. [Q5M8S2-1]
DR CTD; 74306; -.
DR MGI; MGI:1921556; Prss46.
DR VEuPathDB; HostDB:ENSMUSG00000049719; -.
DR eggNOG; KOG3627; Eukaryota.
DR GeneTree; ENSGT00940000162940; -.
DR HOGENOM; CLU_006842_0_4_1; -.
DR InParanoid; Q5M8S2; -.
DR OMA; EICNHRY; -.
DR OrthoDB; 1314811at2759; -.
DR PhylomeDB; Q5M8S2; -.
DR TreeFam; TF351676; -.
DR BioGRID-ORCS; 74306; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Prss46; mouse.
DR PRO; PR:Q5M8S2; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q5M8S2; protein.
DR Bgee; ENSMUSG00000049719; Expressed in spermatid and 5 other tissues.
DR Genevisible; Q5M8S2; MM.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR CDD; cd00190; Tryp_SPc; 1.
DR Gene3D; 2.40.10.10; -; 1.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR001314; Peptidase_S1A.
DR InterPro; IPR001254; Trypsin_dom.
DR InterPro; IPR018114; TRYPSIN_HIS.
DR Pfam; PF00089; Trypsin; 1.
DR PRINTS; PR00722; CHYMOTRYPSIN.
DR SMART; SM00020; Tryp_SPc; 1.
DR SUPFAM; SSF50494; SSF50494; 1.
DR PROSITE; PS50240; TRYPSIN_DOM; 1.
DR PROSITE; PS00134; TRYPSIN_HIS; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Disulfide bond; Hydrolase; Membrane; Protease;
KW Reference proteome; Serine protease; Transmembrane; Transmembrane helix.
FT CHAIN 1..314
FT /note="Serine protease 46"
FT /id="PRO_0000418333"
FT TRANSMEM 293..313
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 44..281
FT /note="Peptidase S1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT ACT_SITE 84
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 130
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 233
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT DISULFID 69..85
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 164..239
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 197..219
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 229..257
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT VAR_SEQ 1..28
FT /note="MACGSVDPHGLLSSPLASARLNSLPYME -> MRATLCIPLSTLPS (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_044025"
SQ SEQUENCE 314 AA; 35185 MW; A6F67DEA4CDB980A CRC64;
MACGSVDPHG LLSSPLASAR LNSLPYMEGP WIWSCGQTNI TCKVVNGKAV EVGKWPWQVS
ILFLGMYICS GSLIHHHWIL TAAHCLQRSK NPAKYTVKVG VQTLPDNSTS ELLVTRIVIH
ENFINRMSDD IAILKLKYPV TWSPLVQPIC LPSFNLKPSI GTMCWVVGWG LEKAEGHPKT
PYSVQGLAVR IVNNEICNHR YQFLLLKNQK KFIGNDMLCT SSEWGLDTCQ DTSGSSLVCQ
MNKTWVQMGV VSWNFDCGRR QFPSVYTSTS HFTQWIKRQI GDLKFTSMAV PSFLSPFILT
GYILLVSLGS LWLL
